Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Trans-chalcone suppresses tumor growth mediated at least in part by the induction of heme oxygenase-1 in breast cancer

  • Komoto, Tatiana Takahasi (Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Lee, Jaehak (Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Lertpatipanpong, Pattawika (Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Ryu, Junsun (Department of Otolaryngology-Head and Neck Surgery, Center for Thyroid Cancer, Research Institute and Hospital, National Cancer Center) ;
  • Marins, Mozart (Biotechnology Unit, University of Ribeirao Preto) ;
  • Fachin, Ana Lucia (Biotechnology Unit, University of Ribeirao Preto) ;
  • Baek, Seung Joon (Laboratory of Signal Transduction, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2020.10.14
  • Accepted : 2021.01.15
  • Published : 2021.10.15
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Abstract

Despite intensive research efforts in recent decades, cancer remains a leading cause of death worldwide. The chalcone family is a promising group of phytochemicals for therapeutic use against cancer development. Naturally-occurring chalcones, as well as synthetic chalcone analogues, have shown many beneficial biological properties, including anti-inflammatory, antioxidant, and anti-cancer activities. In this report, trans-chalcone (TChal) was found to increase cell death in breast cancer cells, assessed using high content screening. Subsequently, using antibody array analysis, TChal was found to increase heme oxygenase-1 (HO-1) expression in TChal-treated breast cancer cells. Blocking of HO-1 by siRNA in breast cancer cells diminished the effect of TChal on cell growth inhibition. TChal-fed mice also showed less tumor growth compared to vehicle-fed mice. Overall, we found that TChal increases HO-1 expression in breast cancer cells, thereby enhancing anti-tumorigenesis. Our results suggest that HO-1 expression could be a potential new target of TChal for anti-tumorigenesis in breast cancer.

Keywords

Acknowledgement

This work was supported by the Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research Center, Seoul National University, and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2018R1A2B2002923). This work was also supported by a clinical research grant (NCC-1810150) provided by the National Cancer Center and by CAPES PhD fellowship and PDSE (process number: 8881.187564/2018-01) granted to Tatiana Takahasi Komoto. Histological analysis was supported by Korea Mouse Phenotyping Center (KMPC).

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