Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of NaF Contents on the NEFA and Uric Acid Concentrations in Rats

NaF 첨가량이 흰쥐의 NEFA 및 Uric Acid 농도에 미치는 영향

  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • 김한수 (부산대학교 식품공학과)
  • Received : 2021.09.17
  • Accepted : 2021.10.28
  • Published : 2021.10.30
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Abstract

The aim of this study was to investigate the hematology and serum chemistry values on Sprague-Dawley rats, used sodium fluoride (NaF) for 5 weeks. Seven-week-old male rats were divided into nine groups and fed experimental diets with oral NaF treatment, basal diet group (BG), basal diet plus oral NaF groups (NF3~NF50). Concentrations of non-esterified fatty acid (NEFA), blood urea nitrogen (BUN), creatinine, uric acid in serum were significantly higher in the oral NaF groups than BG (𝛼=0.05). Total calcium (T-Ca) and phosphorus (Pi) concentrations was observed to be significant difference, it was decreased with an increase in the NaF levels (𝛼=0.05). Accordingly, sodium fluoride exposures and dose-response relationship, which was found on the bad influences in hematology. Such association is considered that the long-term fluoride exposure caused nephrotoxicity.

본 연구는 NaF 경구 투여가 Sprague Dawley계 수컷 흰쥐의 혈청 중 non-esterified fatty acid (NEFA), blood urea nitrogen (BUN), creatinine, uric acid 및 전해질 농도에 미치는 영향을 조사하기 위하여, 기본식이를 급여한 대조군인 BG군과 기본식이에 NaF 경구 투여군(NF3~50군) 9그룹의 식이군으로 나누어 5주간 실험사육을 행하였다. 흰쥐의 NEFA와 BUN, creatinine, uric acid 농도는 NaF 농도에 비례적으로 증가하였으며, 고농도의 NaF 투여군에서 대조군보다 유의적인 차이로 높게 관찰되었다(𝛼=0.05). 한편, 전해질 농도 중 total calcium (T-Ca)과 인(Pi) 농도는 대조군보다 NaF 투여군에서 낮게 동정되었으며, NaF 20 mg 이상 경구 투여한 군에서 유의적인 차이를 보였다(𝛼=0.05). 흰쥐의 NaF 경구 투여 처리는 농도가 증가함에 따라 NEFA 및 BUN, creatinine, uric acid의 농도가 상승하였으며, 전해질 농도는 저하되는 것으로 나타나 신장 기능 장애 등 바람직하지 않은 영향을 주는 것으로 확인되었다.

Keywords

Acknowledgement

본 연구는 부산대학교 기본연구지원사업(2년)에 의하여 수행되었습니다.

References

  1. S. A. Kang, K. H. Jang, K. H. Hong, W. A. Choi, K. H. Jung, I. Y. Lee, "Effects of dietary β-glucan on adiposity and serum lipids levels in obese rats induced by high fat diet", Journal of the Korean Society of Food Science and Nutrition, Vol.31, No.6 pp.1052-1057, (2002). https://doi.org/10.3746/JKFN.2002.31.6.1052
  2. C. P. Wen, K. Matsushita, J. Coresh, K. Iseki, M. Islam, R. Katz, W. McClellan, C. A. Peralta, H. Y. Wang, D. Zeeuw, B. C. Astor, R. T. Gansevoort, A. S. Levey, A. Levin, "Relative risks of chronic kidney disease for mortality and end-stage renal disease across races are similar", Kidney International, Vol.86, No.4 pp.819-827, (2014). https://doi.org/10.1038/ki.2013.553
  3. S. Yusuf, D. Wood, J. Ralston, K. S. Reddy, "The World Heart Federation's vision for worldwide cardiovascular disease prevention", The Lancet, Vol.386, No.9991 pp.399-402, (2015). https://doi.org/10.1016/S0140-6736(15)60265-3
  4. D. Grapov, S. H. Adams, T. L. Pedersen, W. T. Garvey, J. W. Newman, "Type 2 diabetes associated changes in the plasma non-esterified fatty acids", Oxylipins and Endocannabinoids, PloS one, Vol.7, No.11 pp.e48852, (2012). https://doi.org/10.1371/journal.pone.0048852
  5. M. Gil-Campos, M. C. Ramirez Tortosa, C. M. Aguilera, R. Canete, A. Gil, "Fasting and postprandial adiponectin alterations anticipate NEFA and TNF-α changes in prepubertal obese children", Nutrition Metabolism and Cardiovascular Diseases, Vol.21, No.1 pp.62-68, (2011).
  6. T. Yasu, M. Kobayashi, A. Mutoh, K. Yamakawa, S. I. Momomura, S. Ueda, "Dihydropyridine calcium channel blockers inhibit nonesterified fatty acid-induced endothelial and rheological dysfunction". Clinical Science, Vol.125, No.5 pp.247-255, (2013). https://doi.org/10.1042/CS20120311
  7. A. Ziomber, A. Machnik, A. Dahlmann, P. Dietsch, F. X. Beck, H. Wagner, K. F. Hilgers, F. C. Luft, K. U. Eckardt, J. Titze, "Sodium, potassium, chloride, and bicarbonate-related effects on blood pressure and electrolyte homeostasis in deoxycorticosterone acetate-treated rats". American Journal of Physiology-Renal Physiology, Vol.295, No.6 pp.F1752- F1763, (2008). https://doi.org/10.1152/ajprenal.00531.2007
  8. T. Akimoto, C. Ito, M. Kato, M. Ogura, S. Muto, E. Kusano, "Reduced hydration status characterized by disproportionate elevation of blood urea nitrogen to serum creatinine among the patients with cerebral infarction", Medical Hypotheses, Vol.77, No.4 pp.601-604, (2011). https://doi.org/10.1016/j.mehy.2011.06.044
  9. W. C. Lin, H. M. Shih, L. C. Lin, "Preliminary prospective study to assess the effect of early blood urea nitrogen/creatinine ratio-based hydration therapy on poststroke infection rate and length of stay in acute ischemic stroke", Journal of Stroke Cerebrovascular Diseases, Vol.24, No.12 pp.2720-2727, (2015). https://doi.org/10.1016/j.jstrokecerebrovasdis.2015.08.002
  10. J. W. Schrock, M. Glasenapp, K. Drogell, "Elevated blood urea nitrogen/creatinine ratio is associated with poor outcome in patients with ischemic stroke", Clinical Neurology and Neurosurgery, Vol.114, No.7 pp.881-884, (2012). https://doi.org/10.1016/j.clineuro.2012.01.031
  11. S. C. Chen, J. M. Chang, S. M. Yeh, H. M. Su, H. C. Chen, "Association of uric acid and left ventricular mass index with renal outcomes in chronic kidney disease", American Journal of Hypertension, Vol.26, No.2 pp.243-249, (2013). https://doi.org/10.1093/ajh/hps020
  12. M. Duran, E. Ornek, S. N. Murat, M. Turfan, M. A. Vatankulu, A. Ocak, C. Doger, A. A. Yalcin, M. B. Demircelik, "High levels of serum uric acid impair development of coronary collaterals in patients with acute coronary syndrome", Angiology, Vol.63, No.6 pp.472-475, (2012). https://doi.org/10.1177/0003319711422433
  13. F. J. Nieto, C. Iribarren, M. D. Gross, G. W. Comstock, R. G. Cutler, "Uric acid and serum antioxidant capacity: a reaction to atherosclerosis?", Atherosclerosis, Vol.148, No.1 pp.131-139, (2000). https://doi.org/10.1016/S0021-9150(99)00214-2
  14. D. Ghosh, S. Das, R. Maiti, D. Jana, U. B. Das, "Testicular toxicity in sodium fluoride treated rats: association with oxidative stress", Reproductive Toxicology, Vol.16, No.4 pp.385-390, (2002). https://doi.org/10.1016/S0890-6238(02)00038-2
  15. C. H. Turner, I. Owan, E. J. Brizendine, W. Zhang, M. E. Wilson, A. J. Dunipace, "High fluoride intakes cause osteomalacia and diminished bone strength in rats with renal deficiency", Bone, Vol.19, No.6 pp.595-601, (1996). https://doi.org/10.1016/S8756-3282(96)00278-5
  16. Y. N. Wang, K. Q. Xiao, J. L. Liu, G. Dallner, Z. Z. Guan, "Effect of long term fluoride exposure on lipid composition in rat liver", Toxicology, Vol.146, No.2 pp.161-169, (2000). https://doi.org/10.1016/S0300-483X(00)00167-0
  17. X. Xiong, J. Liu, W. He, T. Xia, P. He, X. Chen, K. Yang, A. Wang, "Dose-effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children", Environmental Research, Vol.103, No.1 pp.112-116, (2007). https://doi.org/10.1016/j.envres.2006.05.008
  18. H. S. Kim, "Effects of various sodium fluoride (NaF) administration on blood glucose, lipid composition and A/G ratio in rats", Journal of Korean Applied Science and technology, Vol.35, No.3 pp. 826-835, (2018).
  19. H. S. Kim, "Influences of sodium fluoride contents on hepatic functional enzyme activities in rats", Journal of Environmental Science International, Vol.28, No.11 pp. 943-950, (2019). https://doi.org/10.5322/JESI.2019.28.11.943
  20. H. S. Kim, "Influences of the BUN and creatinine level by krill (Euphausia superba) meal and NaF administration in rats", Journal of Korean Applied Science and technology, Vol.35, No.3 pp. 848-856, (2018)
  21. E. Ritz, M. L. Gross, R. Dikow, "Role of calcium-phosphorous disorders in the progression of renal failure", Kidney International, Vol.68, pp.S66-S70, (2005).
  22. D. A. Lieberman, J. Ghormley, K. Flora, "Effect of oral sodium phosphate colon preparation on serum electrolytes in patients with normal serum creatinine", Gastrointestinal Endoscopy, Vol.43, No.5 pp.467-469, (1996). https://doi.org/10.1016/S0016-5107(96)70287-0
  23. M. Tanaka, F. Suzuki, Y. Seko, T. Hara, Y. Kawamura, H. Sezaki, S. Saitoh, "Renal dysfunction and hypophosphatemia during long-term lamivudine plus adefovir dipivoxil therapy in patients with chronic hepatitis B", Journal of Gastroenterology, Vol.49, No.3 pp.470-480, (2014). https://doi.org/10.1007/s00535-013-0779-0
  24. R. J. Johnson, D. H. Kang, D. Feig, S. Kivlighn, J. Kanellis, S. Watanabe, K. R. Tuttle, B. Rodriguez-Iturbe, J. HerreraAcosta, M. Mazzali, "Is there a pathogenetic role for uric acid in hypertension and cardiovascular and renal disease?". Hypertension, Vol.41, No.6 pp.1183-1190, (2003). https://doi.org/10.1161/01.hyp.0000069700.62727.c5
  25. F. Leyva, S. Anker, J. W. Swan, I. F. Godsland, C. S. Wingrove, T. P. Chua, J. C. Stevenson, A. J. S. Coats, "Serum uric acid as an index of impaired oxidative metabolism in chronic heart failure", European Heart Journal, Vol.18, No.5 pp.858-865, (1997). https://doi.org/10.1093/oxfordjournals.eurheartj.a015352
  26. M. C. Odden, A. R. Amadu, E. Smit, L. Lo, C. A. Peralta, "Uric acid levels, kidney function, and cardiovascular mortality in US adults: National Health and Nutrition Examination Survey (NHANES) 1988-1994 and 1999-2002", American Journal of Kidney Diseases, Vol.64, No.4 pp.550-557, (2014). https://doi.org/10.1053/j.ajkd.2014.04.024
  27. M. Volterrani, F. Iellamo, B. Sposato, F. Romeo, "Uric acid lowering therapy in cardiovascular diseases", International Journal of Cardiology, Vol.213, pp.20-22, (2016). https://doi.org/10.1016/j.ijcard.2015.08.088
  28. C. M. Aguilera, M. Gil-Campos, R. Canete, A. Gil, "Alterations in plasma and tissue lipids associated with obesity and metabolic syndrome", Clinical Science, Vol.114, No.3 pp.183-189, (2008). https://doi.org/10.1042/cs20070115
  29. C. Gayet, E. Bailhache, H. Dumon, L. Martin, B. Siliart, P. Nguyen, "Insulin resistance and changes in plasma concentration of TNFα, IGF1, and NEFA in dogs during weight gain and obesity", Journal of Animal Physiology Animal Nutrition, Vol.88, No.3-4 pp.157-165, (2004). https://doi.org/10.1111/j.1439-0396.2003.00473.x
  30. J. J. Zwiazek, J. M. Shay, "The effects of sodium fluoride on cytoplasmic leakage and the lipid and fatty acid composition of jack pine (Pinus banksiana) seedlings", Canadian Journal of Botany, Vol.66, No.3 pp.535-541, (1988). https://doi.org/10.1139/b88-076
  31. E. Grucka-Mamczar, E. Birkner, S. Kasperczyk, A. Kasperczyk, D. Chlubek, D. Samujlo, A. Ceglowska, "Lipid balance in rats with fluoride-induced hyperglycemia", Fluoride, Vol.37, No.3 pp.195-200, (2004).
  32. B. H. Kang, H. Y. Son, C. S. Ha, H. S. Lee, "References values of hematology and serum chemistry in Ktc: Sprague-Dawley rats", Korean Journal of Laboratory Animal Science, Vol.11, No.2 pp.141-145, (1995).
  33. L. R. Narasimhan, W. Goodman, C. K. N. Patel, "Correlation of breath ammonia with blood urea nitrogen and creatinine during hemodialysis", Proceedings of National Academy Sciences, Vol.98, No.8 pp.4617-4621, (2001). https://doi.org/10.1073/pnas.071057598
  34. D. Aronson, M. A. Mittleman, A. J. Burger, "Elevated blood urea nitrogen level as a predictor of mortality in patients admitted for decompensated heart failure", The American Journal of Medicine, Vol.116, No.7 pp.466-473, (2004). https://doi.org/10.1016/j.amjmed.2003.11.014
  35. M. Sinha, P. Manna, P. C. Sil, "Aqueous extract of the bark of Terminalia arjuna plays a protective role against sodiumfluoride-induced hepatic and renal oxidative stress", Journal of Natural Medicines, Vol.61, No.3 pp.251-260, (2007). https://doi.org/10.1007/s11418-007-0133-z
  36. M. E. Goldberg, J. Cantillo, G. E. Larijani, M. Torjman, D. Vekeman, H. Schieren, "Sevoflurane versus isoflurane for maintenance of anesthesia: are serum inorganic fluoride ion concentrations of concern?", Anesthesia and Analgesia, Vol.82, No.6 pp.1268-1272, (1996). https://doi.org/10.1213/00000539-199606000-00029
  37. X. Guo, Y. Qin, K. Zheng, M. Gong, J. Wu, W. Shou, X. Cheng, X. Liangyu, E. Xu, X. Li, L. Qiu, "Improved glomerular filtration rate estimation using new equations combined with standardized cystatin C and creatinine in chinese adult chronic kidney disease patients", Clinical Biochemistry, Vol.47, No.13 pp.1220- 1226, (2014). https://doi.org/10.1016/j.clinbiochem.2014.05.060
  38. K. Julier, R. da Silva, C. Garcia, L. Bestmann, P. Frascarolo, A. Zollinger, T. Pasch, "Preconditioning by sevoflurane decreases biochemical markers for myocardial and renal dysfunction in coronary artery bypass graft surgery: a double-blinded, placebo-controlled, multicenter study". The Journal of American Society and Anesthesiology, Vol.98, No.6 pp.1315-1327, (2003).
  39. T. Nishiyama, A. Hirasaki, "Effects of sevoflurane anaesthesia on renal function duration of administration and area under the curve and rate of decrease of serum inorganic fluoride", European Journal of Anaesthesiology, Vol.12, No.5 pp.477-482, (1995).