Journal of the Korean Society of Manufacturing Process Engineers (한국기계가공학회지)

The Korean Society of Manufacturing Process Engineers (한국기계가공학회)
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Cell Disruption of Dunaliella salina using Batch Low Frequency Non-Focused Ultrasound

비집속 회분저주파를 이용한 Dunaliella salina 세포 파쇄

  • 최준혁 (조선대학교 기계공학과) ;
  • 김광호 (한국폴리텍대학 광주캠퍼스 기계시스템과) ;
  • 박종락 (조선대학교 광기술공학과) ;
  • 정상화 (조선대학교 기계공학과)
  • Received : 2021.06.29
  • Accepted : 2021.08.24
  • Published : 2021.10.30
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Abstract

Using fossil fuels in existing industrial systems causes a variety of social problems. Recently, many studies have been conducted on bio-refineries, which aim to actively utilize biomass to reduce the use of fossil fuels and solve various social problems. Among them, research using microalgae as a third-generation biomass has attracted considerable attention. Microalgae use inorganic matter to produce organic matter, and cell destruction is necessary to extract useful organic materials from microalgae. The extracted organic materials are currently used in various industrial fields. Numerous cell-destruction methods exist. We have investigated cell disruption by sonication, especially its efficiency. Ultrasound is a sound wave with frequencies above 20 kHz, and destroys cells by sending high energy through a cavitation that occurs, according to the characteristics of the sound wave. The Dunaliella salina microalgae used in this study was cultured in a flat-type photobioreactor. Experiments were performed using a batch low-frequency processing device. Logistic model was applied to analyze the results of cell-destruction experiments using ultrasound. The proper conditions for the most efficient cell destruction were OD 1.4(microalgae concentration)), 54watt(output power) and 200mL(microalgae capacity).

Keywords

Acknowledgement

이 논문은 2021년 한국연구재단의 이공학 개인기초 연구지원사업의 지원을 받아 수행되었음.(Grant No. 2018R1D1A1B07047810).

References

  1. Kim, G. H., Lee, D. W., Jeong, S. H., "Scale-up of Flat Panel Photobioreactor considering Hydrodynamics," The Korea Society of Manufacturing Process Engineers, Vol. 17, No.1 pp. 48-56, 2018. https://doi.org/10.14775/ksmpe.2018.17.1.048
  2. Kim, H. W., "Realization of microalgal growth by CO2 and biofuel production plant construcion by bioreactor," Korea Institute of Plane Engineering and Construction, Vol. 13, No. 2, pp. 5-15, 2017.
  3. Rahimi, M., Mohamadian, E., Dadari, S., Arbab, M. M., Karimi, N., "Application of high frequency ultrasound in different irradiation systems for photosynthesis pigment extraction from Chlorella microalgae," Korean Journal of Chemical Engineering, Vol. 34, No. 4, pp. 1100-1108, 2017. https://doi.org/10.1007/s11814-016-0336-7
  4. Bae, M. G., Choi, J. H., Park, J. R., Jeong, S. H., "Cell Disruption of Microalgae by Low-Frequency Non-Focused Ultrasound," The Korea Society of Manufacturing Process Engineers, Vol. 19, No. 2, pp. 105-112, 2020.
  5. Spengler, J. F., Coakley, W. T., and Christensen, K. T., "Microstreaming effects on particle concentration in an ultrasonic standing wave," AIChE journal, Vol. 49, No. 11, pp. 2773-2782, 2003. https://doi.org/10.1002/aic.690491110
  6. Gunerken, E., D'Hondt, E., Eppink, M. H. M., Garcia-Gonzlez, L., Elst, K. and Wijffels, R. H "Cell disruption for microalgae biorefineries," Biotechnology Advances, Vol. 33, No. 2, pp. 243-260, 2015. https://doi.org/10.1016/j.biotechadv.2015.01.008
  7. Mason, Timothy J., "Ultrasound in synthetic organic chemistry," Chemical Society Reviews, Vol. 26, pp. 443-451, 1997. https://doi.org/10.1039/CS9972600443
  8. Kim, J. H., Yoon, C. M. and Jeong, S. H., "On-off Control for Continuous Culture of Microalgae in Flat Panel Photobioreactor," Journals of Korean Society of Manufacturing Technology Engineers, Vol. 25, No. 3, pp. 237-243, 2016. https://doi.org/10.7735/ksmte.2016.25.3.237
  9. Mytilinaios, I., Salih, M., Schofield, H. K., Lambert, R. J. W., "Growth curve prediction from optical density data," International Journal of Food Microbiology, Vol. 154, No. 3, pp. 169-176, 2012. https://doi.org/10.1016/j.ijfoodmicro.2011.12.035
  10. Choi, J. H., "A Study on the Cell Membrane Disruption of Microalgae Dunaliella salina by Ultrasound Sonication," A Thesis for a Master's degree, Chosun University, Republic of Korea, 2021.
  11. Zwietering, M. H., Jongenburger, I., Rombouts, F. M., RIET, K. VAN 'T, "Modeling of the bacterial growth curve," Applied and environmental microbiology, Vol. 56, No. 6, pp. 1875-1881, 1990. https://doi.org/10.1128/aem.56.6.1875-1881.1990
  12. Jiang, Y. and Duan, Z., "Comparison of Four Quantitative Techniques for Monitoring Microalgae Disruption by Low-Frequency Ultrasound and Acoustic Energy Efficiency," Environmental Science & Technology, Vol. 52, No. 5, pp. 3295-3303, 2018. https://doi.org/10.1021/acs.est.7b05896