Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Prevalence and Intensity of Opisthorchis viverrini Metacercarial Infection in Fish from Phnom Penh, Takeo, and Kandal Provinces, Cambodia

  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Choi, Seung-Ho (Society of Korean Naturalist, Institute of Ecology and Conservation) ;
  • Jung, Bong-Kwang (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Hong, Sooji (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Ryoo, Seungwan (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Chang, Taehee (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Keon Hoon (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Na, Byoung-Kuk (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Hong, Sung-Jong (Convergence Research Center for Insect Vectors, Incheon National University) ;
  • Khieu, Virak (National Center for Parasitology, Entomology and Malaria Control) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion)
  • Received : 2021.10.09
  • Accepted : 2021.10.11
  • Published : 2021.10.31
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Abstract

The prevalence and intensity of Opisthorchis viverrini metacercariae (OvMc) were investigated in fish from 3 southern administrative regions along the Mekong River in Cambodia, i.e., Phnom Penh, Takeo, and Kandal Provinces from 2017 to 2020. A total of 295 freshwater fish (24 species) were transported to our laboratory with ice and examined using the artificial digestion method. In Phnom Penh, among 4 fish species positive for OvMc, 9 (23.7%) of 38 specimens examined were infected, and their intensity of infection averaged 4.3 metacercariae per infected fish. In Takeo Province, among 10 fish species positive for OvMc, 24 (38.1%) out of 63 fish examined were infected, and their intensity of infection was av. 14.4 metacercariae per infected fish. In particular, all of 3 Osteochilus schlegelii fish examined were infected, and their infection intensity was high, 34.7 metacercariae per fish. In Kandal Province, among 6 fish species positive for OvMc, 46 (90.2%) out of 51 specimens examined were infected, and their infection intensity was 24.0 metacercaraie per infected fish. All fish of Systomus orphoides (n=17), Barbonymus altus (n=14), and Rasbora aurotaenia (n=2) were infected, and their intensity of infection averaged 37.7, 21.6, and 18.5 metacercariae per fish, respectively. Metacercariae of Haplochis yokogawai, Haplorchis taichui, and Centrocestus formosanus were detected in fish from Takeo and Kandal Provinces. From these results, it has been confirmed that a variety of fish species from Phnom Penh, Takeo, and Kandal Provinces are commonly infected with OvMc, and preventive measures to avoid human O. viverrini infection should be performed in Cambodia.

Keywords

Acknowledgement

We thank Jung-A Kim and Hee-Ju Kim in the Department of Parasitology, Gyeongsang National University College of Medicine, Jinju, Korea, for their help in fish examinations. We also thank the staff of the Korea Association of Health Promotion, Seoul, Republic of Korea, who participated in the Korea-Cambodia Cooperation Project on Parasite Control in Cambodia (2017, 2018, and 2020).

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