Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Paeoniae Radix Alba on a thioacetamide induced liver fibrosis mice model

Thioacetamide로 유발된 간섬유증 동물 모델에서 백작약이 미치는 효능

  • Lee, Se Hui (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seo, Bu-Il (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.05.12
  • Accepted : 2021.07.26
  • Published : 2021.10.31
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Abstract

This study investigated the anti-fibrotic and antioxidant effects of Paeonia Radix Alba water extract (PR) on thioacetamide (TAA)-induced liver fibrosis in a mouse model and its underlying mechanisms. Liver fibrosis was induced by intraperitoneal injection of TAA (three times a week) for 8 weeks. Furthermore, silymarin (50 mg/kg body weight) and PR (200 mg/kg body weight) were administered for 8 weeks. PR treatment downregulated aspartate aminotransferase (AST), alanine aminotransferase (ALT), ammonia, and myeloperoxidase levels. Moreover, PR treatment downregulated NOX2 and p47phox and upregulated antioxidant enzymes by activating the Nrf2/Keap1 signaling pathway. Furthermore, PR inhibited the factors associated with fibrosis, such as α-SMA and collagen I. AMPK/SIRT1 was upregulated by PR treatment. Overall, these results suggest that PR attenuates liver fibrosis by regulating the Nrf2/Keap1 and AMPK/SIRT1/NF-κB signaling pathways through the inhibition of oxidative stress. Hence, PR has potential as a remedy for preventing and treating liver fibrosis.

본 연구에서 in vitro를 통해 백작약 열수 추출물의 total polyphenol, total flavonoid 함량, DPPH 및 ABTS radical 소거능 측정으로 높은 항산화능을 확인했으며, 백작약 열수 추출물 투여로 인한 AST, ALT, ammonia 및 MPO 수치의 호전을 확인하였다. 또한 간 조직을 이용한 western blot 실시 후 모든 인자에서 유의적인 차이를 확인하였다. 이 결과를 토대로, 백작약 열수 추출물이 TAA로 인한 산화적 스트레스를 Nrf2/Keap1 경로의 활성화를 통해 SIRT1/AMPK/NF-kBp65를 조절하였으며, 섬유화 관련 단백질의 발현을 억제함으로써 간섬유증에 대한 백작약 열수 추출물의 긍정적인 효과가 있음을 제시하였다. 따라서 선행 연구를 통해 TAA로 유발한 급성 간손상 동물 모델에서 간보호 효과를 나타낸 백작약 열수 추출물이 TAA로 유발한 간섬유증 동물 모델에서 산화적 스트레스 억제와 간 기능 개선을 나타냈으며 향후 후보 소재로서 가능성이 있다고 판단된다.

Keywords

Acknowledgement

이 논문은 2021년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2018R1A5A2025272).

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