Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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The Convergence Effect of Histamine and Atropine on Intestinal Contractility

위장관 수축성에 대한 Histamine과 Atropine의 융합성 조절 효과

  • Je, Hyun Dong (Dept. of Pharmacology, College of Pharmacy, Daegu Catholic University) ;
  • Min, Young Sil (Dept. of Pharmaceutical Science, Jungwon University)
  • 제현동 (대구가톨릭대학교 약학대학) ;
  • 민영실 (중원대학교 의약바이오학과)
  • Received : 2021.08.13
  • Accepted : 2021.10.20
  • Published : 2021.10.28
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Abstract

The aim of the study was to observe the influence and related mechanism of histamine and its analogues used for hypersensitivity tests and used as an indicator of impurities in drugs on the tissue-specific intestinal contraction. Intestinal contraction includes the activation of thick or thin filament regulation. However, there are few reports addressing the question whether this regulation is involved in histamine-induced regulation. We hypothesized that histamine plays a role in tissue-dependent regulation of intestinal contractility. Denuded ileal/colonic longitudinal and circular muscles of male rats were used and isometric contractions were recorded using a data acquisition system. Interestingly, histamine alone didn't increase the contraction of the circular muscle but increased the contraction of the longitudinal muscle. Histamine together with atropine (M3 receptor antagonist) didn't inhibit the contraction of the longitudinal and circular muscle. Therefore, histamine alone and together with atropine increases the ileal longitudinal muscle contraction suggesting that additional mechanisms (decreased receptor density, postreceptor signaling or distribution of agonists) might be involved in the regulation of ileal muscle contractility. In conclusion, histamine and/or atropine has some effect on the regulation of the longitudinal contractility regardless of M3 receptor and the simpler test would be preferred as the drug impurity test compared to more complicated tests.

본 연구에서 진단, 과민반응 검사에 사용되며 의약품에 불순물로 포함되어 규제되는 histamine의 단독 및 병용 투여에서 조직/근육 선택적 장관 수축성 조절에 대한 실험을 하고자 하였다. Histamine은 muscarinic receptor 작용 외에 histamine H1 receptor에 대한 직접 작용으로 수축성을 증가시켰고 회장/결장 윤주근에서 수축성을 거의 증가시키지 않았다. 또한 M3 수용체에 선택적으로 작용하는 atropine의 병용 효과를 관찰하였는데 histamine과 병용된 atropine은 장관 중 회장의 종주근의 수축성을 거의 감소시키지 않았고 회장/결장의 윤주근의 수축성을 감소시키지 않았다. 따라서 적어도 회장의 종주근에서 histamine은 M3 수용체를 거의 경유하지 않고 작용하고 atropine은 항무스카린 효과 외에 histamine에 의한 조직 선택적 위장관 운동성을 거의 조절하지 않는 것으로 생각된다. 그리고 이러한 단순한 검색은 의약품 불순물 검사로 선호될 것으로 생각된다.

Keywords

References

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