Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Comparative analysis of hydroponically cultivated barley sprouts yield, polyphenol and mineral content by nutrient solution treatment

양액 처리에 따른 수경재배 새싹보리 수량과 폴리페놀 및 무기질 함량 비교분석

  • Kim, Kyeoung Cheol (Major in Plant Resource and Environment, SARI, Jeju National University) ;
  • Kim, Ju-Sung (Major in Plant Resource and Environment, SARI, Jeju National University)
  • 김경철 (제주대학교 식물자원환경전공) ;
  • 김주성 (제주대학교 식물자원환경전공)
  • Received : 2021.07.13
  • Accepted : 2021.07.25
  • Published : 2021.09.30
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Abstract

Barley (Hordeum vulgare cv. Hangmaeg) sprouts are important microgreens that contain high levels of polyphenols and flavonoids, as well as minerals, vitamin and chlorophyll. Barley sprouts were grown for 9 days and growth was checked every 3 days. In this study, the cultivation efficiency according to the nutrient solution treatment was evaluated by analyzing the length of barley sprouts, fresh weight, chlorophyll, and the yield by growth period. In addition, we tried to increase the industrial applicability of germinated barley through analysis of inorganic component, total polyphenol and total flavonoid content of the extract, and functional substance analysis using HPLC. As a result, the growth rate of the nutrient solution treatment group was faster than that of the control group. When harvested on the 9th day of sowing, the nutrient solution treatment group showed a significant increase in yield compared to the control group. And the barley sprout extract of the nutrient solution treatment group had higher total flavonoid content and luteolin content. Also, the efficiency of water was higher than that of ethanol when extracting phenolics from barley sprouts. Therefore, this study suggests that nutrient input is effective for increasing polyphenol content and increasing production in barley sprout hydroponics.

본 연구는 생육 기간에 따른 새싹보리 식물체의 길이, 무게 및 클로로필 함량 분석과 수확량을 통해 양액 처리에 따른 재배 효율성을 평가하고, 생산된 새싹보리의 무기성분 분석과 용매별 추출물의 총 폴리페놀 및 총플라보노이드 함량과 HPLC를 이용한 기능성 물질 분석을 통해 새싹보리의 산업적 이용 가능성을 높이고자 하였다. 새싹보리의 생육에서 무처리구보다 양액 처리구에서 생육속도가 빨랐으며 특히 지상부 길이가 크게 신장하였다. 지상부 길이가 더 증가한 만큼 생체중 또한 증가하였지만 수분 흡수량의 차이보다는 건물중의 증가로 이어졌다. 이는 수확량의 차이로 나타났다. 무처리구와 비교하였을 때 양액 처리구에서 생산량이 유의적으로 증가하였다. 클로로필 함량 분석을 통해 생육 9일차에서 광합성을 통한 독립영양 과정을 확인하였고, 생육 3일차와 6일차에서 양액 처리를 통한 무기성분 투입이 초기 생장에 보조적인 효과가 있다고 판단된다. 새싹보리의 폴리페놀 함량 분석 결과 총 폴리페놀 함량은 무처리구에서 높았지만 총플라보노이드 함량과 luteolin 함량은 양액 처리에 의해 증가하였다. 또한 추출 용매를 물로 사용하였을 때 폴리페놀 추출에 효과적이었다. 따라서 새싹보리 수경재배에서 양액의 투입이 높은 폴리페놀을 가진 새싹보리를 생산하고 생산량 증대에도 효과가 있음을 시사한다.

Keywords

Acknowledgement

본 논문은 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 사회맞춤형 산학협력 선도대학(LINC+) 육성사업의 연구결과입니다.

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