The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Aging effects on the diurnal patterns of gut microbial composition in male and female mice

  • Kim, Hyun-Jung (Department of Pharmacology, College of Medicine, Ewha Womans University) ;
  • Moon, Chang Mo (Department of Internal Medicine, College of Medicine, Ewha Womans University) ;
  • Kang, Jihee Lee (Inflammation-Cancer Microenvironment Research Center, College of Medicine, Ewha Womans University) ;
  • Park, Eun-Mi (Department of Pharmacology, College of Medicine, Ewha Womans University)
  • Received : 2021.06.09
  • Accepted : 2021.09.07
  • Published : 2021.11.01
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Abstract

Composition of the gut microbiota changes with aging and plays an important role in age-associated disease such as metabolic syndrome, cancer, and neurodegeneration. The gut microbiota composition oscillates through the day, and the disruption of their diurnal rhythm results in gut dysbiosis leading to metabolic and immune dysfunctions. It is well documented that circadian rhythm changes with age in several biological functions such as sleep, body temperature, and hormone secretion. However, it is not defined whether the diurnal pattern of gut microbial composition is affected by aging. To evaluate aging effects on the diurnal pattern of the gut microbiome, we evaluated the taxa profiles of cecal contents obtained from young and aged mice of both sexes at daytime and nighttime points by 16S rRNA gene sequencing. At the phylum level, the ratio of Firmicutes to Bacteroidetes and the relative abundances of Verrucomicrobia and Cyanobacteria were increased in aged male mice at night compared with that of young male mice. Meanwhile, the relative abundances of Sutterellaceae, Alloprevotella, Lachnospiraceae UCG-001, and Parasutterella increased in aged female mice at night compared with that of young female mice. The Lachnospiraceae NK4A136 group relative abundance increased in aged mice of both sexes but at opposite time points. These results showed the changes in diurnal patterns of gut microbial composition with aging, which varied depending on the sex of the host. We suggest that disturbed diurnal patterns of the gut microbiome can be a factor for the underlying mechanism of age-associated gut dysbiosis.

Keywords

Acknowledgement

This work was supported by the intramural research program through the Ewha Education and Research for Infection funded by Ewha Womans University Medical Center (201900580001), Ewha Alumni Medical Research Grant, and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2019R1A2C1087035 and NRF-2020R1A5A2019210).

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