The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Taxonomic Re-evaluation of Korean Gyromitra Species Based on Morphological and Phylogenetical Studies

  • Cho, Sung-Eun (Forest Biodiversity Division, Korea National Arboretum) ;
  • Kwag, Young-Nam (Forest Biodiversity Division, Korea National Arboretum) ;
  • Han, Sang-Kuk (Forest Biodiversity Division, Korea National Arboretum) ;
  • Kim, Chang Sun (Forest Biodiversity Division, Korea National Arboretum)
  • Received : 2021.07.26
  • Accepted : 2021.09.27
  • Published : 2021.09.30
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Abstract

Several species of Gyromitra are highly poisonous when consumed raw the presence of gyromitrin. In this study, Gyromitra specimens deposited in the Korea National Arboretum were re-examined and subjected to molecular sequence analysis. G. gigas, G. aff. perlata, and G. tianshanensis were identified based on their morphological features and molecular phylogenetic analysis for the first time in Korea. Furthermore, internal transcribed spacer and 28S rRNA sequences of G. esculenta and G. infula were analyzed to confirm their phylogeny.

Keywords

Acknowledgement

We appreciate the efforts of Ho-Pil Choi providing collections for this study. This research was supported by the research fund of the Korea National Arboretum (Project No.: KNA 1-3-3, 20-3).

References

  1. Michelot D, Toth B. Poisoning by Gyromitra esculenta - a review. J Appl Toxicol 1991;11:235-43. https://doi.org/10.1002/jat.2550110403
  2. Miller AN, Yoon A, Gulden G, Stensholt O, Vooren NV, Ohenoja E, Methven AS. Studies in Gyromitra I: the Gyromitra gigas species complex. Mycol Prog 2020;19:1459-1473. https://doi.org/10.1007/s11557-020-01639-8
  3. Wang X-C, Zhuang W-Y. A three-locus phylogeny of Gyromitra (Discinaceae, Pezizales) and discovery of two cryptic species. Mycologia 2018;111:69-77. https://doi.org/10.1080/00275514.2018.1515456
  4. Crous PW et al. Fungal planet description sheets: 716-784. Persoonia 2018;40:240-392. https://doi.org/10.3767/persoonia.2018.40.10
  5. Anonymous. 2013. List of mushrooms in Korea. The Korean Society of Mycology, Seoul, Korea.
  6. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications (Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds). Academic Press, San Diego: 1990;315-22.
  7. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999;41:95-8.
  8. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  9. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Bol Evol 2013;30:772-80. https://doi.org/10.1093/molbev/mst010
  10. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), 25 14 Nov 2010, New Orleans, Louisiana 2010;1-8.
  11. Vooren NV. Typification of Gyromitra perlata, type-species of the subgenus Discina (Discinaceae). Ascomycete.ogr. 2017;9:19-22.