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Unreported Endophytic Fungi Isolated from the Leaves of Evergreen Woody Plants in Korea: Muyocopron lithocarpi and Didymocyrtis cladoniicola

  • Gwon, Ju-Hui (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Lee, Jong-Chul (Department of Biology Education, Korea National University of Education) ;
  • Choi, Jae-Wook (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • Received : 2021.06.07
  • Accepted : 2021.09.17
  • Published : 2021.09.30

Abstract

In this study, endophytic fungi were isolated from the leaves of evergreen woody plants in Hansando Island in South Korea. The fungal isolates were identified based on morphological characteristics and phylogenetic analysis of the DNA sequences of the internal transcribed spacer and the large subunit regions of rDNA. It was confirmed that two endophytic fungal species, Muyocopron lithocarpi and Didymocyrtis cladoniicola, isolated in this study have not previously been reported in South Korea.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902202).

References

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Villar R, Ruiz-Robleto J, Ubera JL, Poorter H. Exploring variation in leaf mass per area (LMA) from leaf to cell: an anatomical analysis of 26 woody species. Am J Bot 2013;100:1969-80. https://doi.org/10.3732/ajb.1200562
  3. Liu CH, Liu TT, Yuan FF, Gu YC. Isolating endophytic fungi from evergreen plants and determining their antifungal activities. Afr J Microbiol Res 2010;4:2243-8.
  4. Matsumura E, Fukuda K. A comparison of fungal endophytic community diversity in tree leaves of rural and urban temperate forests of Kanto district, eastern Japan. Fungal Biol 2013;117:191-201. https://doi.org/10.1016/j.funbio.2013.01.007
  5. Park H, Eom AH. Three unreported endophytic fungi isolated from conifer leaves of Pinus densiflora in Korea. Kor J Mycol 2019;47:35-42. https://doi.org/10.4489/KJM.20190005
  6. Kim CK, Eo JK, Eom AH. Diversity of foliar endophytic fungi isolated from Lindera obtusiloba in Korea. Kor J Mycol 2012;40:136-40. https://doi.org/10.4489/KJM.2012.40.3.136
  7. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  8. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol Res 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  9. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  10. Mapook A, Hyde KD, Dai DQ, Li J, Jones EG, Bahkali AH, Boonmee S. Muyocopronales, ord. nov.,(Dothideomycetes, Ascomycota) and a reappraisal of Muyocopron species from northern Thailand. Phytotaxa 2016;265:225-37. https://doi.org/10.11646/phytotaxa.265.3.3
  11. Diederich P, Kocourkova J, Etayo J, Zhurbenko M. The lichenicolous Phoma species (coelomycetes) on Cladonia. Lichenologist 2007;39:153-63. https://doi.org/10.1017/S0024282907006044
  12. Adams SJ, Robicheau BM, LaRue D, Browne RD, Walker AK. Foliar endophytic fungi from the endangered eastern mountain avens (Geum peckii, Rosaceae) in Canada. Plants 2021;10:1026. https://doi.org/10.3390/plants10051026
  13. Trakunyingcharoen T, Lombard L, Groenewald JZ, Cheewangkoon R, Toanun C, Alfenas AC, Crous PW. Mycoparasitic species of Sphaerellopsis, and allied lichenicolous and other genera. IMA fungus 2014;5:391-414. https://doi.org/10.5598/imafungus.2014.05.02.05
  14. Piccolo SL, Mondello V, Giambra S, Conigliaro G, Torta L, Burruano S. Arthrinium phaeospermum, Phoma cladoniicola and Ulocladium consortiale, new Olive pathogens in Italy. J Phytopathol 2014;162:258-63. https://doi.org/10.1111/jph.12179