DOI QR코드

DOI QR Code

Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing Material for Concrete Repair by Atmospheric and Room Temperature Plasma Mutagenesis and Response Surface Methodology

  • Han, Pei-pei (State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology) ;
  • Geng, Wen-ji (State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology) ;
  • Li, Meng-nan (State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology) ;
  • Jia, Shi-ru (State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology) ;
  • Yin, Ji-long (Tianjin Research Institute for Water Transportation Engineering, M.O.T.) ;
  • Xue, Run-ze (Tianjin Research Institute for Water Transportation Engineering, M.O.T.)
  • 투고 : 2021.05.20
  • 심사 : 2021.07.28
  • 발행 : 2021.09.28

초록

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Next, we investigated the optimal biomineralization conditions and precipitation crystal form using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/l calcium chloride, 45 g/l urea, reaction temperature of 45℃, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method to engineer biocementing material for concrete repair.

키워드

과제정보

The study was funded by the Key Technologies R&D Program of Tianjin (No. 20YFZCSN00910).

참고문헌

  1. Muynck WD, Cox K, Belie ND, Verstraete W. 2008. Bacterial carbonate precipitation as an alternative surface treatment for concrete. Constr. Build. Mater. 22: 875-885. https://doi.org/10.1016/j.conbuildmat.2006.12.011
  2. Pacheco-Torgala F, Labrincha JA. 2013. Biotech cementitious materials: Some aspects of an innovative approach for concrete with enhanced durability. Constr. Build. Mater. 40: 1136-1141. https://doi.org/10.1016/j.conbuildmat.2012.09.080
  3. Seifan M, Berenjian A. 2018. Application of microbially induced calcium carbonate precipitation in designing bio self-healing concrete. World J. Microbiol. Biotechnol. 34: 168. https://doi.org/10.1007/s11274-018-2552-2
  4. Tait K, Sayer JA, Gharieb MM, Gadd GM. 1999. Fungal production of calcium oxalate in leaf litter microcosms. Soil Biol. Biochem. 31: 1189-1192. https://doi.org/10.1016/S0038-0717(99)00008-5
  5. Seifan M, Samani AK, Berenjian A. 2016a. Bioconcrete: next generation of self-healing concrete. Appl. Microbiol. Biotechnol. 100: 2591-2602. https://doi.org/10.1007/s00253-016-7316-z
  6. Anderson S, Appanna VD, Huang J, Viswanatha T. 1992. A novel role for calcite in calcium homeostasis. FEBS Lett. 308: 94-96. https://doi.org/10.1016/0014-5793(92)81059-U
  7. Achal V, Mukherjee A, Reddy MS. 2011. Effect of calcifying bacteria on permeation properties of concrete structures. J. Ind. Microbiol. Biotechnol. 38: 1229-1234. https://doi.org/10.1007/s10295-010-0901-8
  8. Dhami NK, Mukherjee A, Reddy MS. 2016. Applicability of bacterial biocementation in sustainable construction materials. Asia-Pac J. Chem. Eng. 11: 795-802. https://doi.org/10.1002/apj.2014
  9. Li M, Zhu X, Mukherjee A, Huang M, Achal V. 2017. Biomineralization in metakaolin modified cement mortar to improve its strength with lowered cement content. J. Hazard Mater. 329: 178-184. https://doi.org/10.1016/j.jhazmat.2017.01.035
  10. Knoll AH. 2003. Biomineralization and evolutionary history. Rev. Mineral. Geochem. 54: 329-356. https://doi.org/10.2113/0540329
  11. Dick J, Windt WD, Graef BD, Saveyn H, Meeren PVD, Verstraete W. 2006. Biodeposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegradation 17: 357-367. https://doi.org/10.1007/s10532-005-9006-x
  12. Muynck WD, Belie ND, Verstraete W. 2010. Microbial carbonate precipitation in construction materials: A review. Ecol. Eng. 36: 118-136. https://doi.org/10.1016/j.ecoleng.2009.02.006
  13. Tobler DJ, Cuthbert MO, Greswell RB, Riley MS, Renshaw JC, Handley-Sidhu S, et al. 2011. Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite. Geochim. Cosmochim. Acta 75: 3290-3301. https://doi.org/10.1016/j.gca.2011.03.023
  14. Okyay TO, Rodrigues DF. 2014. Optimized carbonate micro-particle production by Sporosarcina pasteurii using response surface methodology. Ecol. Eng. 62: 168-174. https://doi.org/10.1016/j.ecoleng.2013.10.024
  15. Seifan M, Samani AK, Berenjian A. 2016. Induced calcium carbonate precipitation using Bacillus species. Appl. Microbiol. Biotechnol. 100: 9895-9906. https://doi.org/10.1007/s00253-016-7701-7
  16. Steinberg DM, Bursztyn D. 2010. Response surface methodology in biotechnology. Qual. Eng. 22: 78-87. https://doi.org/10.1080/08982110903510388
  17. Zhang X, Zhang XF, Li HP, Wang LY, Zhang C, Xing XH, et al. 2014. Atmospheric and room temperature plasma as a new powerful mutagenesis tool. Appl. Microbiol. Biotechnol. 98: 5387-5396. https://doi.org/10.1007/s00253-014-5755-y
  18. Ramachandran SK, Ramakrishnan V, Bang SS. 2001. Remediation of concrete using microorganisms. Aci Mater. J. 98: 3-9.
  19. Whiffin VS, Paassen LA, Harkes MP. 2007. Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol. J. 24: 417-423. https://doi.org/10.1080/01490450701436505
  20. Kalil SJ, Maugeri F, Rodrigues MI. 2000. Response surface analysis and simulation as a tool for bioprocess design and optimization. Process Biochem. 35: 539-550. https://doi.org/10.1016/S0032-9592(99)00101-6
  21. Mori Y, Enomae T, Isogai A. 2009. Preparation of pure vaterite by simple mechanical mixing of two aqueous salt solutions. Mat. Sci. Eng. 29: 1409-1414. https://doi.org/10.1016/j.msec.2008.11.009
  22. Kontoyannis CG, Vagenas NV. 2000. Calcium carbonate phase analysis using XRD and FTIR spectroscopy. Analyst 125: 251-255. https://doi.org/10.1039/a908609i
  23. Liu R, Liang L, Ma J, Ren X, Jing M, Chen K, et al. 2013. An engineering Escherichia coli, mutant with high succinic acid production in the defined medium obtained by the atmospheric and room temperature plasma[J]. Process Biochem. 48: 1603-1609. https://doi.org/10.1016/j.procbio.2013.07.020
  24. Hua X, Wang J, Wu Z, Zhang H. 2010. A salt tolerant Enterobacter cloacae, mutant for bioaugmentation of petrolecum- and salt-contaminated soil[J]. Biochem. Eng. J. 49: 201-206. https://doi.org/10.1016/j.bej.2009.12.014
  25. Gebauer D, Gunawidjaja PN, Ko JY, Bacsik Z, Aziz B, Liu L, et al. 2010. Proto-calcite and proto-vaterite in amorphous calcium carbonates. Angew. Chem. Int. Ed. Engl. 49: 8889-8891. https://doi.org/10.1002/anie.201003220
  26. Andersen FA, Brecevic L. 1991. Infrared spectra of amorphous and crystalline calcium carbonate. Acta Chem. Scand. 45:1018-1024. https://doi.org/10.3891/acta.chem.scand.45-1018
  27. Morita R. 1980. Calcite precipitation by marine bacteria. Geomicrobiol. J. 2: 63-82. https://doi.org/10.1080/01490458009377751
  28. Chen Y, Cheng JJ, Creamer KS. 2008. Inhibition of anaerobic digestion process: a review. Bioresour. Technol. 99: 4044-4064. https://doi.org/10.1016/j.biortech.2007.01.057
  29. Sanchez-Roman M, Rivadeneyra MA, Vasconcelos C, Mckenzie JA. 2010. Biomineralization of carbonate and phosphate by moderately halophilic bacteria. FEMS Microbiol. Ecol. 61: 273-284. https://doi.org/10.1111/j.1574-6941.2007.00336.x
  30. Yang LQ, Wang SH, Tian YP. 2010. Purification, properties, and application of a novel acid urease from Enterobacter sp. Appl. Biochem. Biotechnol. 160: 303-313. https://doi.org/10.1007/s12010-008-8159-6
  31. Huang SC, Burne RA, Chen YYM .2014. The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by cody. Appl. Environ. Microbiol. 80: 5386-5393. https://doi.org/10.1128/AEM.00755-14
  32. Huang SC, Chen YYM. 2016. Role of vicrkx and glnr in pH-dependent regulation of the Streptococcus salivarius 57.1 urease operon. mSphere 1: e00033-16.
  33. Benini S, Rypniewski WR, Wilson KS, Miletti S, Ciurli S, Mangani S. 1999. A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels. Structure 7: 205-216. https://doi.org/10.1016/S0969-2126(99)80026-4
  34. Kakelar MM, Ebrahimi S. 2016. Up-scaling application of microbial carbonate precipitation: optimization of urease production using response surface methodology and injection modification. Int. J. Environ. Sci. Technol. 13: 2619-2628. https://doi.org/10.1007/s13762-016-1070-8
  35. Okwadha GD, Li J. 2010. Optimum conditions for microbial carbonate precipitation. Chemosphere 81: 1143-1148. https://doi.org/10.1016/j.chemosphere.2010.09.066
  36. Bundur ZB, Kirisits MJ, Ferron RD. 2015. Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength. Cem. Concr. Res. 67: 237-245. https://doi.org/10.1016/j.cemconres.2014.10.002

피인용 문헌

  1. Biomineralization Induced by Cells of Sporosarcina pasteurii: Mechanisms, Applications and Challenges vol.9, pp.11, 2021, https://doi.org/10.3390/microorganisms9112396