Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Two Enteropathogenic Escherichia coli Strains Representing Novel Serotypes and Investigation of Their Roles in Adhesion

  • Wang, Jing (TEDA Institute of Biological Sciences and Biotechnology, Nankai University) ;
  • Jiao, HongBo (LanLing Center for Disease Control and Prevention) ;
  • Zhang, XinFeng (Taian Center for Disease Control and Prevention) ;
  • Zhang, YuanQing (Jinan KeJia Medical Laboratory, Inc.) ;
  • Sun, Na (Shandong Center for Disease Control and Prevention) ;
  • Yang, Ying (Shandong Center for Disease Control and Prevention) ;
  • Wei, Yi (TEDA Institute of Biological Sciences and Biotechnology, Nankai University) ;
  • Hu, Bin (Shandong Center for Disease Control and Prevention) ;
  • Guo, Xi (TEDA Institute of Biological Sciences and Biotechnology, Nankai University)
  • Received : 2021.05.13
  • Accepted : 2021.07.14
  • Published : 2021.09.28
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Abstract

Enteropathogenic Escherichia coli (EPEC), which belongs to the attaching and effacing diarrheagenic E. coli strains, is a major causative agent of life-threatening diarrhea in infants in developing countries. Most EPEC isolates correspond to certain O serotypes; however, many strains are non-typeable. Two EPEC strains, EPEC001 and EPEC080, which could not be serotyped during routine detection, were isolated. In this study, we conducted an in-depth characterization of their putative O-antigen gene clusters (O-AGCs) and also performed constructed mutagenesis of the O-AGCs for functional analysis of O-antigen (OAg) synthesis. Sequence analysis revealed that the occurrence of O-AGCs in EPEC001 and E. coli O132 may be mediated by recombination between them, and EPEC080 and E. coli O2/O50 might acquire each O-AGC from uncommon ancestors. We also indicated that OAg-knockout bacteria were highly adhesive in vitro, except for the EPEC001 wzy derivative, whose adherent capability was less than that of its wild-type strain, providing direct evidence that OAg plays a key role in EPEC pathogenesis. Together, we identified two EPEC O serotypes in silico and experimentally, and we also studied the adherent capabilities of their OAgs, which highlighted the fundamental and pathogenic role of OAg in EPEC.

Keywords

Acknowledgement

This work was supported by the Shandong Medical and Health Science and Technology Development Programs [Grant No. 2017WS455], the Shandong Preventive Medicine Association Zhifei Disease Prevention and Control Technology Research Fund Project [Grant No. LYH2017-03], and a grant from the Tianjin Municipal Natural Science Foundation [Grant No. 17JCYBJC24300].

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