Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Quantitative Analysis of Chemical Constituents in Codonopsis lanceolata

  • Ju, Yeongdon (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Jeon, Jeong Wook (Nutricare Co Ltd) ;
  • Hyun, Kyung-Yae (Department of Clinical Laboratory Science, Dong-Eui University)
  • Received : 2021.08.13
  • Accepted : 2021.09.14
  • Published : 2021.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Codonopsis lanceolata has numerous chemical constituents that includes polyphenols, saponins, tannins, triterpene, alkaloids, and steroids. The extract of C. lanceolata was partitioned with Haxane, CH2Cl2, EtOAc, n-BuOH and MeOH. The determination of structure for lancemaside G, lancemaside B, lancemaside A were based on physicochemical and HPLC chromatogram data, including NMR and HR-MS. In addition, tangshenoside I and lobetyolin were identified in the material separation process for the extract of C. lanceolata, and content analysis was performed using HPLC. The compounds were confirmed as lancemaside G, lancemaside B, lancemaside A, tangshenoside I, and lobetyolin.

Keywords

Acknowledgement

This research was supported by 2020 Regional Specialized Industry Promotion (Non-R&D) Project of Hongcheon Institute of Medicinal Herb (HIMH) and Brain Busan 21 Plus (BB21+) Project.

References

  1. Byeon SE, Choi WS, Hong EK, Lee J, Rhee MH, Park HJ, et al. Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses. Arch Pharm Res. 2009. 32: 813-822. https://doi.org/10.1007/s12272-009-1601-7
  2. Choi MS, Choi PS. Plant Regeneration and Saponin Contents in Codonopsis lanceolata L.. Korean J Med Crop Sci. 1999. 7: 275-281.
  3. Chung KH, Jo HJ, Yoon JA, Song BC, An JH. Free Radical-scavenging Activities of Amaranth (Amaranthus spp. L.) Seed Extracts. Food Eng Prog. 2014. 18: 116-123. https://doi.org/10.13050/foodengprog.2014.18.2.116
  4. Han AY, Lee YS, Kwon SH, Lee HS, Lee KW, Seol GH. Codonopsis lanceolata extract prevents hypertension in rats. Phytomedicine. 2018. 15: 119-124.
  5. Hossen MJ, Kim MY, Kim JH, Cho JY. Codonopsis lanceolata: A Review of Its Therapeutic Potentials. Phytother Res. 2016. 30: 347-356. https://doi.org/10.1002/ptr.5553
  6. Hwang BS, Kim JY, Jang M, Kim GC, Park YH, Hwang IG. Quantitative Analysis of Tangshenoside I and Lobetyolin from Korean Deoduk (Codonopsis lanceolata). Korean J. Food Nutr. 2018. 31: 957-963. https://doi.org/10.9799/KSFAN.2018.31.6.957
  7. Ichikawa M, Ohta S, Komoto N, Ushijima M, Kodera Y, Hayama M, et al. Simultaneous determination of seven saponins in the roots of Codonopsis lanceolata by liquid chromatography-mass spectrometry. J Nat Med. 2009. 63: 52-57. https://doi.org/10.1007/s11418-008-0294-4
  8. Joh EH, Kim DH. Lancemaside A inhibits lipopolysaccharide-induced inflammation by targeting LPS/TLR4 complex. J Cell Biochem. 2010. 111: 865-871. https://doi.org/10.1002/jcb.22773
  9. Kim E, Yang WS, Kim JH, Park JG, Kim HG, Ko J, et al. Lancemaside A from Codonopsis lanceolata modulates the inflammatory responses mediated by monocytes and macrophages. Mediators Inflamm. 2014. 2014: 405158.
  10. Kim JA, Moon HK, Choi YE. Triterpenoid Saponin Contents of the Leaf, Stem and Root of Codonopsis lanceolata. Korean J Med Crop Sci. 2014. 22: 1-7. https://doi.org/10.7783/KJMCS.2014.22.1.1
  11. Kim NY, Chae HS, Lee IS, Kim DS, Seo KT, Park SJ. Analysis of Chemical Composition and Antioxidant Activity of Codonopsis lanceolata Skin. J Korean Soc Food Sci Nutr. 2010. 11: 1627-1633.
  12. Kim SS, Jeong MH, Seo YC, Kim JS, Kim NS, Woon WB, et al. Comparison of Antioxidant Activities by High Pressure Extraction of Codonopsis lanceolata from Different Production Areas. Korean J Med Crop Sci. 2010. 18: 248-254.
  13. Lee MJ, Nam JH, Um IE, Kang CK, Rho IR. Determination the optimum extraction method for saponin lancemasides in Codonopsis lanceolata. Korean J Food Sci Technol. 2019. 51: 103-108. https://doi.org/10.9721/KJFST.2019.51.2.103
  14. Shim SB, Chun YJ. The Study on Skin Safety and Efficacy of Codonopsis lanceolata Root Fermentation Extract. JKAIS. 2012. 11: 5623-5627.
  15. Shin YK, Han AY, Hsieh YS, Kwon SH, Kim JH, Lee KW, et al. Lancemaside A from Codonopsis lanceolata prevents hypertension by inhibiting NADPH oxidase 2-mediated MAPK signalling and improving NO bioavailability in rats. J Pharm Pharmacol. 2019. 71: 1458-1468. https://doi.org/10.1111/jphp.13140
  16. Shirota O, Nagamatsu K, Sekita S, Komoto N, Kuroyanagi M, Ichikawa M, et al. Preparative separation of the saponin Lancemaside a from Codonopsis lanceolata by centrifugal partition chromatography. Phytochem Anal. 2008. 19: 403-410. https://doi.org/10.1002/pca.1065
  17. Ushijima M, Komoto N, Sugizono Y, Mizuno I, Sumihiro M, Ichikawa M, et al. Triterpene glycosides from the roots of Codonopsis lanceolata. Chem Pharm Bull. 2008. 56: 308-314. https://doi.org/10.1248/cpb.56.308