대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제47권3호
  • /
  • Pages.213-218
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  • 2021
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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AhR 발현을 통한 콤부차 분획물의 안티폴루션 효과 연구

A Study on the Anti-Pollution Effect of Kombucha Fraction through AhR Expression

  • 강은빈 (대구한의대학교 화장품약리학과) ;
  • 현진아 (대구한의대학교 화장품약리학과) ;
  • 권현지 (대구한의대학교 화장품약리학과) ;
  • 범석현 (대구한의대학교 화장품약리학과) ;
  • 한동근 (대구한의대학교 화장품약리학과) ;
  • 박하은 ((주)허니스트) ;
  • 김현정 ((주)허니스트) ;
  • 곽기성 ((주)허니스트) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Kang, Eun-Bin (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Hyun, Jin-A (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kwon, Hyun-Ji (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Beom, Seok-Hyun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Han, Dong-Geun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Park, Ha-Eun (R&D Center, HONEST.Co.,Ltd.) ;
  • Kim, Hyun-Jeong (R&D Center, HONEST.Co.,Ltd.) ;
  • Kwak, Ki-Sung (R&D Center, HONEST.Co.,Ltd.) ;
  • An, Bong-Jeon (Department of Cosmeceutical Science, Daegu Hanny University)
  • 투고 : 2021.05.24
  • 심사 : 2021.07.21
  • 발행 : 2021.09.30
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초록

본 연구에서는 설탕을 넣은 녹차나 홍차에 유익균을 첨가해 발효시킨 음료인 콤부차를 컬럼 크로마토그래피를 이용해 분획한 후, TLC를 통해 플라보노이드류인 퀘르세틴 글리코사이드(quercetin glycoside)의 유효성분 유무를 확인하고 그 분획물을 이용하여 공해로부터 피부를 보호 및 개선하는 안티폴루션 효과를 확인하였다. 인간 각질형성세포에 Kombucha-quercetin glycoside (K-QG)를 처리하여 세포 생존율을 확인한 결과, 100 ㎍/mL 농도까지 90% 이상의 생존율을 보였으며, 안티폴루션 효과를 보기 위해 benzo[e]pyrene과 미세먼지 자극에 의한 세포 생존율을 측정한 결과, 100 ㎍/mL에서 각각 68.79%, 50.68%의 개선율을 보였다. 또한, benzo[a]pyrene에 의해 활성화되는 aryl hydrocarbon receptor (AhR) 발현을 웨스턴 블롯을 통해 확인한 결과, 대조군에 대비하여 100 ㎍/mL에서 31.08%의 억제율을 보였다. 본 연구의 결과를 통해 K-QG는 벤조피렌과 미세먼지로부터 자극받은 피부를 보호, 개선해주며 안티폴루션 기능성 소재로써 가치가 있는 것으로 사료된다.

In this study, Kombucha, a fermented drink by adding beneficial bacteria to green tea or black tea with sugar, was classified using column chromatography, and the presence or absence of quercetin glycoside, a type of flavonoids, was confirmed through TLC. In addition, the anti-pollution effect of protecting and improving the skin from pollution was confirmed using the fraction. Keratinocytes were treated with K-QG to confirm the cell viability, showing a survival rate of 90% or more up to a concentration of 100 ㎍/mL, and the cell viability by benzo[e]pyrene and fine dust stimulation to see the anti-pollution effect was measured. At 100 ㎍/mL, the improvement rates were about 68.79% and 50.68%, respectively. In addition, as a result of confirming the expression of AhR activated by benzo[a]pyrene through Western blot, it showed an inhibition rate of about 31.08% at 100 ㎍/mL compared to the control group. Through the results of this study, K-QG is thought to be valuable as an anti-pollution functional material, protecting and improving skin irritated from benzopyrene and fine dust.

키워드

과제정보

본 연구는 중소벤처기업부의 위기지역중소기업 Scale-up R&D사업(과제번호: GB2020-64)의 지원에 의해 수행되었으며 이에 감사드립니다.

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