Applied Microscopy

  • Volume 51
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  • Pages.12.1-12.12
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  • 2021
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
Korean Society of Microscopy (한국현미경학회)
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Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

  • Barry G. H. Janssen (Department of Medical Biophysics, Western University) ;
  • Mohamadreza Najiminaini (Imaging Program, St.Joseph's Health Care, Lawson Health Research Institute) ;
  • Yan Min Zhang (Kidney Clinical Research Unit (KCRU), London Health Sciences Centre) ;
  • Parsa Omidi (Imaging Program, St.Joseph's Health Care, Lawson Health Research Institute) ;
  • Jeffrey J. L. Carson (Department of Medical Biophysics, Western University)
  • Received : 2021.02.12
  • Accepted : 2021.06.08
  • Published : 2021.12.31
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Abstract

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

Keywords

Acknowledgement

This work was sponsored by the Western University and the Lawson Health Research Institute.

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