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The Korean Society of Phycology (한국조류학회(藻類))
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Downregulation of PyHRG1, encoding a novel secretory protein in the red alga Pyropia yezoensis, enhances heat tolerance

  • Han, Narae (Department of Biology Education, Chonnam National University and Khumho Research Institute) ;
  • Wi, Jiwoong (Department of Biology Education, Chonnam National University and Khumho Research Institute) ;
  • Im, Sungoh (Department of Biology Education, Chonnam National University and Khumho Research Institute) ;
  • Lim, Ka-Min (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hun-Dong (Department of Biology, Chonnam National University) ;
  • Jeong, Won-Joong (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Geun-Joong (Department of Biology, Chonnam National University) ;
  • Kim, Chan Song (Fisheries Seed and Breeding Research Institute, National Institute of Fisheries Science) ;
  • Park, Eun-Jeong (Fisheries Seed and Breeding Research Institute, National Institute of Fisheries Science) ;
  • Hwang, Mi Sook (Fisheries Seed and Breeding Research Institute, National Institute of Fisheries Science) ;
  • Choi, Dong-Woog (Department of Biology Education, Chonnam National University and Khumho Research Institute)
  • Received : 2021.07.08
  • Accepted : 2021.08.26
  • Published : 2021.09.15
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Abstract

An increase in seawater temperature owing to global warming is expected to substantially limit the growth of marine algae, including Pyropia yezoensis, a commercially valuable red alga. To improve our knowledge of the genes involved in the acquisition of heat tolerance in P. yezoensis, transcriptomes sequences were obtained from both the wild-type SG104 P. yezoensis and heat-tolerant mutant Gy500. We selected 1,251 differentially expressed genes that were up- or downregulated in response to the heat stress condition and in the heat-tolerant mutant Gy500, based on fragment per million reads expression values. Among them, PyHRG1 was downregulated under heat stress in SG104 and expressed at a low level in Gy500. PyHRG1 encodes a secretory protein of 26.5 kDa. PyHRG1 shows no significant sequence homology with any known genes deposited in public databases to date. However, PyHRG1 homologs were found in other red algae, including other Pyropia species. When PyHRG1 was introduced into the single-cell green alga Chlamydomonas reinhardtii, transformed cells overexpressing PyHRG1 showed severely retarded growth. These results demonstrate that PyHRG1 encodes a novel red algae-specific protein and plays a role in heat tolerance in algae. The transcriptome sequences obtained in this study, which include PyHRG1, will facilitate future studies to understand the molecular mechanisms involved in heat tolerance in red algae.

Keywords

Acknowledgement

This works was supported by the Korean Institute of Planning and Evaluation for Technology, Agriculture, Forestry and Fisheries (IPET) through a Golden Seed Project (Project number, 213008-05-4-SB830), funded by the Ministry of Oceans and Fisheries (MOF) and a grant (R2021013) from the National Institute of Fisheries Science, Republic of Korea.

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