Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Modulation of Fermented Lotus Root on Pacemaker Potentials in Interstitial Cells of Cajal of Murine Small Intestine

생쥐 소장 카할세포 조절에 발효 연근의 효능 연구

  • Park, Dong Suk (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Kim, Jeong Nam (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Kwon, Hyo Eun (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Kwon, Min Ji (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Park, Eun-Jung (Department of Food and Nutrition, Gachon University) ;
  • Lee, Hae-Jeung (Department of Food and Nutrition, Gachon University) ;
  • Kim, Byung Joo (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
  • 박동석 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김정남 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 권효은 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 권민지 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 박은정 (가천대학교 식품영양학과) ;
  • 이해정 (가천대학교 식품영양학과) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학부)
  • Received : 2021.06.30
  • Accepted : 2021.08.15
  • Published : 2021.08.31
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Abstract

Obejectives : The purpose of this study is to find out the efficacy of pacemaker potentials of interstitial Cells of Cajal (ICC) by Fermented Lotus Root (FLR) in small intestine. Methods : Enzyme digestions were used to separate the ICC. Using electrophysiological methods, pacemaker potentials were measured and intestinal transit rates (ITR) experiments were conducted to identify in vivo gastrointestinal motility. Results : 1. FLR (0.5-10 mg/ml) caused membrane depolarization by electrophysiological methods. 2. In the case of pretreatment with a Ca2+ free solution and thapsigargin, the pacemaker potential disappeared and in this case, FLR did not have a membrane depolarization reaction. 3. Lowering the concentration of extracellular Na+ concentration stoped the pacemaker potentials and inhibited the reaction caused by FLR. Flufenamic acid also inhibited the reaction by FLR. 4. In mice, ITR was increased by FLR. Conclusions : This study shows that FLR can control ICC by an internal/external Ca2+ and Na+. It also shows that FLR can be a good candidate for gastrointestinal motility medication development.

Keywords

Acknowledgement

This study was supported by Korea Food Research Institute (E0210400-01), Republic of Korea.

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