Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Endoplasmic Reticulum Stress Induces CAP2 Expression Promoting Epithelial-Mesenchymal Transition in Liver Cancer Cells

  • Yoon, Sarah (Department of Physiology, Ajou University School of Medicine) ;
  • Shin, Boram (Department of Physiology, Ajou University School of Medicine) ;
  • Woo, Hyun Goo (Department of Physiology, Ajou University School of Medicine)
  • Received : 2021.02.03
  • Accepted : 2021.05.23
  • Published : 2021.08.31
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Abstract

Cyclase-associated protein 2 (CAP2) has been addressed as a candidate biomarker in various cancer types. Previously, we have shown that CAP2 is expressed during multi-step hepatocarcinogenesis; however, its underlying mechanisms in liver cancer cells are not fully elucidated yet. Here, we demonstrated that endoplasmic reticulum (ER) stress induced CAP2 expression, and which promoted migration and invasion of liver cancer cells. We also found that the ER stress-induced CAP2 expression is mediated through activation of protein kinase C epsilon (PKCε) and the promotor binding of activating transcription factor 2 (ATF2). In addition, we further demonstrated that CAP2 expression promoted epithelial-mesenchymal transition (EMT) through activation of Rac1 and ERK. In conclusion, we suggest that ER stress induces CAP2 expression promoting EMT in liver cancer cells. Our results shed light on the novel functions of CAP2 in the metastatic process of liver cancer cells.

Keywords

Acknowledgement

This work was supported by grants from the National Research Foundation of Korea (NRF), funded by the Korean government (MSIP) (NRF-2017R1E1A1A01074733, NRF-2017M3A9B6061509, NRF-2017M3C9A6047620, NRF-2019R1A5A2026045, and NRF-2019R1I1A1A01057206).

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