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Microbial Risk Assessment for Mixed Vegetable Salad and Fresh and Frozen Fruits Distributed in Korea

국내 유통 중인 혼합채소샐러드 및 신선·냉동과일의 미생물 오염실태 조사

  • Park, Hyun-Jin (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Lee, Jeong-Eun (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Sol-A (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Shim, Won-Bo (Department of Food Science & Technology, Gyeongsang National University)
  • 박현진 (경상국립대학교 응용생명과학부) ;
  • 이정은 (경상국립대학교 응용생명과학부) ;
  • 김솔아 (경상국립대학교 응용생명과학부) ;
  • 심원보 (경상국립대학교 식품공학과)
  • Received : 2021.07.29
  • Accepted : 2021.08.25
  • Published : 2021.08.30

Abstract

In this study, the microbial levels on mixed vegetable salads, fresh fruits, and frozen fruits distributed in Korea were comparatively analyzed by food group, region, and quarter. Samples were collected from big markets in large cities from 2018 to 2019 and used for microbiological analysis. The levels of aerobic bacteria for mixed vegetable salads, fresh fruits, and frozen fruits were 6.48, 5.07, and 3.78 log CFU/g, respectively. As a result of analyzing the quarterly contamination levels of aerobic bacteria, the first quarter contamination level was 5.12 log CFU/g while the second quarter showed 6.26 log CFU/g, the third quarter 5.73 log CFU/g, and the fourth quarter 4.42 log CFU/g. A higher number of aerobic bacteria was observed in the second and third quarters when the temperature was higher. There was no difference in the number of bacteria by region. The levels of the coliform group were 1.98 - 3.93 log CFU/g in all samples, and Escherichia coli was detected at 1.38 log CFU/g in 3 out of 27 mixed vegetable salads. Since the mixed vegetable salad and fresh fruit used in this study exceeded the standard (3 log CFU/g) for unheated foods and E. coli was detected in three fresh fruits, stricter hygiene management in the manufacturing stage of salads and fresh fruit is required.

국내에서 유통되고 있는 혼합채소샐러드류, 신선과일 및 냉동과일에 대해 미생물 오염 도를 식품군별, 지역별, 분기별로 비교 분석하였다. 식품군 별로 일반세균수는 혼합채소샐러드에서 가장 높은 6.48 log CFU/g, 신선과일은 5.07 log CFU/g, 냉동과일은3.78 log CFU/g 수준으로 나타났다. 분기별 일반세균수를 비교 분석한 결과에서는 1분기 오염도는 5.12 log CFU/g, 2분기는 6.26 log CFU/g, 3분기는 5.73 log CFU/g, 4분기는 4.42 log CFU/g으로 확인되어 외부온도가 높은 2,3분기에서 조금 더 높은 일반 세균수가 관찰되었다. 지역별 일반세균수의 오염은 5.26-5.47 log CFU/g으로 수준으로 지역별 차이는 확인되지 않았다. 대장균군의 경우 1.98-3.93 log CFU/g으로 나타났고, E. coli의 경우 혼합채소샐러드 27점 중 3점에서 평균 1.38 log CFU/g으로 검출되었다. 이러한 결과를 종합할 때 혼합채소샐러드와 신선과일은 Solberg 등이 제시한 비가열 식품의 일반세균 기준치(3 log CFU/g)를 초과한 것으로 확인되었고, 3점의 신선과일 시료에서 대장균이 검출되어 샐러드류 및 신선과일의 원료의 안전성과 제조 및 유통 단계에서의 위생관리가 보다 철저히 수행되어야 할 것으로 판단된다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 공동연구사업(과제번호: PJ015283)과 2020년도 중소벤처기업부의 기술개발사업 지원에 의한 연구임[S2901659] 연구개발비 지원에 의해 수행되었으며 이에 감사드립니다.

References

  1. Kim, H.Y., Oh, S.W., Chung, S.Y., Choi, S.H., Lee, J.W., Yang, J.Y., Seo, E.C., Kim, Y.H., Park, H.O., Yang, C.Y., Ha, S.C., Shin, I.S., An investigation of microbial contamination of ready-to-eat products in Seoul, Korea. Korean J. Food Sci. Technol., 43, 39-44 (2011). https://doi.org/10.9721/KJFST.2011.43.1.039
  2. Park, G.J., Chun, S.J., Park, K.H., Hong, C.H., Kim, J.W., Survey on the foodborne illness experience and awareness of food safety practice among Korean consumers. J. Food Hyg. Saf., 18, 139-145 (2003).
  3. Kim, T.S., Kim, M.J., Kang, Y.M., Oh, G.N., Choi, S.Y., Oh, M.S., Yang, Y.S., Seo, J.M., Ryu, M.G., Kim, E.S., Ha, D.R., Cho, B.S., Molecular characterization and toxin profile of bacillus cereus strains isolated from ready-to-eat foods. Korean J. Food Sci. Technol., 46, 334-340 (2014). https://doi.org/10.9721/KJFST.2014.46.3.334
  4. Alves, D., McEwen, B., Hazlett, M., Maxie, G., Anderson, N. Trends in bovine abortions submitted to the Ontario Ministry of Agriculture, Food and Rural Affairs, 1993-1995. The Canadian Veterinary Journal, 37, 287 (1996).
  5. Shin, S.W., Rew, K., Kwak, T.K., Hazard analysis of packaged meals (Dosirak) during delivery. J. Food Hyg. Saf., 5, 85-98 (1990).
  6. Heaton, J.C., Jones, K., Microbial contamination of fruit and vegetables and the behaviour of enteropathogens in the phyllosphere: A review. J. Appl. Microbiol., 104, 613-626 (2008) https://doi.org/10.1111/j.1365-2672.2007.03587.x
  7. Park, S.Y., Yeon, J.H., Choi, J.W., Lee, M.J., Lee, D.H., Kim, K.S., Park, K.H., Ha, S.D., Assessment of contamination levels of foodborne pathogens isolated in major RTE foods marketed in convenience stores. Korean J. Food Sci. Technol., 37, 274-278 (2005).
  8. Sivapalasingam S., Friedman, C.R., Cohen, L., Tauxe, R.V., Fresh produce: A growing cause of outbreak of foodborne illness in the United States, 1973 through 1997. J. Food Prot., 67, 2342-2353 (2004). https://doi.org/10.4315/0362-028X-67.10.2342
  9. Jung, J., Oh, K.K., Seo, S.M., Yang, S., Jung, K.S., Roh, E., Ryu, J.G., Distribution of Foodborne Pathogens from Garlic Chives and Its Production Environments in the Southern Part of Korea. J. Food Hyg. Saf., 35, 477-488 (2020). https://doi.org/10.13103/JFHS.2020.35.5.477
  10. Kim, S.J., Sun, S.H., Kim, G.C., Kim, H.R., Yoon, K.S., Quality changes of fresh-cut leafy and condiment vegetables during refrigerated storage. J. Korean Soc. Food Sci. Nutr., 40, 1141-1149 (2011). https://doi.org/10.3746/jkfn.2011.40.8.1141
  11. Centers for Disease Control and Prevention, (2021, JULY 15). Multistate outbreak of E. coli O157:H7 infections linked to romain lettuce. Retrieved from: www.cdc.gov/ecoli/2018/0157h7-04-18
  12. Soriano, J.M., Rico, H., Molto, J.C., Manes, J., Incidence of microbial flora in lettuce, meat and Spanish potato omelette from restaurants. Food Microbiol., 18, 159-163 (2001). https://doi.org/10.1006/fmic.2000.0386
  13. Oh, D. H., Ding, T., Ha, S. D., Bahk, G. J., The risk estimation of Listeria monocytogenes for ready-to-eats fresh cutvegetables. J. Food Hyg. Saf., 24, 50-55 (2009).
  14. Minsistry of Food and ?rug Safety, (2021, JULY 15). microbiological test method, http://www.foodsafetykorea.go.kr/foodcode/01_03.jsp?idx=362.
  15. Abadias, M., Usall, J., Anguera, M., Solsona, C., Vinas, I., Microbiological quality of fresh, minimally-processed fruitand vegetables, and sprouts from retail establishments. Int. J. Food Microbiol., 123, 121-129 (2008). https://doi.org/10.1016/j.ijfoodmicro.2007.12.013
  16. Song, B.R., Kim, S.H., Kim, J.K., Han, J.A., Kwak, H.S., Chung, K.T., Heo, E.J., Establishment of microbial criteria by investigation of microbial contamination in ready-to-eat foods. J. Food Hyg. Saf., 32, 348-354 (2017). https://doi.org/10.13103/JFHS.2017.32.5.348
  17. Jo, M.J., Jeong, A.R., Kim, H.J., Lee, N.R., Oh, S.W., Kim, Y.J., Koo, M.S., Microbiological quality of fresh-cut produce and organic vegetables. Korean J. Food Sci. Technol., 43, 91-97 (2011). https://doi.org/10.9721/KJFST.2011.43.1.091
  18. Lee, B.H., Kim, I.H., Huh, K.S., Cho, K.D., Application of HACCP system on establishing hygienic standards in pizza specialty restaurant-focused on salad items. J. Korean Home Econ. Assoc., 41, 101-116 (2003).
  19. Kang, T.M., Cho, S.K., Park, J.Y., Song, K.B., Chung, M.S., Park, J.H., Analysis of microbial contamination of sprouts and fresh-cut salads in a market. Korean J. Food Sci. Technol., 43, 490-494 (2011). https://doi.org/10.9721/KJFST.2011.43.4.490
  20. Seo, K.Y., Lee, M.J., Yeon, J.H., Kim, I.J., Ha, J.H., Ha, S.D., Microbiological contamination levels of in salad and side dishes distribution in markets. J. Food Hyg. Saf., 21, 263-268 (2006).
  21. Solberg, M., Buchalew, J.J., Chen, C.M., Schaffner D.W., O'Neill, K., McDowell, J., Post, L.S., Boderck, M., Microbial safety assurance system for foodservice facilities. Food Technol., 44, 68-73 (1990).
  22. Lim, G.S., Koo, M.S., Kim, H.J., Kho, Y.H., Park, K.S., Oh, S.W., Determination of statistical sampling plans for bacillus cereus in salad and Kimbab. J. Food Hyg. Saf., 29, 16-20 (2014). https://doi.org/10.13103/JFHS.2014.29.1.016
  23. Shim, S.B., Ham, S.N., Kwoun, P.S., Lee, S.O., Kim, S.H., Lee, G.W., Bang, O.K., Monitoring contamination of vegetable salad and study in reduction for food poisoning. Annu. Rep. KFDA, 7, 364-365 (2003).
  24. Kim, H.K., Lee, H.T., Kim, J.H., Lee, S.S., Analysis of microbiological contamination in ready-to-eat foods. J. Food Hyg. Saf., 23, 285-290 (2008).
  25. Choi, J.W., Park, S.Y., Yeon, J.H., Lee, M.J., Chung, D.H., Lee, K.H., Kim, M.G., Lee, D.H., Kim, K.S., Ha, S.D., Microbial contamination levels of fresh vegetables distributed in markets. J. Food Hyg. Saf., 20, 43-47 (2005).
  26. Park, H.J., Lee, J.E., Kim, S.A., Shim, W.B., Changes in internal and external temperature and microbiological contamination depending on consumer behavior after purchase of fresh-cut produces. J. Food Hyg. Saf., 35, 459-467 (2020). https://doi.org/10.13103/JFHS.2020.35.5.459
  27. Jung, S.H., Hur, M.J., Ju, J.H., Kim, K.A., Oh, S.S., Go, J.M., Im, J.S., Microbiological evaluation of raw vegetables. J. Food Hyg. Saf., 21, 250-257 (2006).