BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development

  • Kim, Byeong-Seong (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Ko, Hyo Rim (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Hwang, Inwoo (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine) ;
  • Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan, College of Medicine) ;
  • Ahn, Jee-Yin (Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine)
  • 투고 : 2021.02.16
  • 심사 : 2021.03.08
  • 발행 : 2021.08.31
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초록

ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.

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과제정보

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government [Ministry of Science, Information and Communication Technology and Future Planning (MSIP)] (2016R1A5A2945889 and 2020R1A2C2003268).

참고문헌

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