Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of immunohistochemical analysis on sinus augmentation using demineralized tooth graft and bovine bone

  • Sohn, Dong-Seok (Department of Dentistry and Oral and Maxillofacial Surgery, School of Medicine, Daegu Catholic University) ;
  • Kim, Ji-Rak (Department of Oral Medicine, School of Dentistry, Kyungpook National University) ;
  • Kim, Hyung-Gyun (Department of Dentistry and Oral and Maxillofacial Surgery, School of Medicine, Daegu Catholic University) ;
  • Choi, Hyun-Suk (Department of Dentistry and Prosthodontics, School of Medicine, Daegu Catholic University) ;
  • Moon, Yong-Suk (Department of Anatomy, School of Medicine, Daegu Catholic University)
  • Received : 2021.03.15
  • Accepted : 2021.07.08
  • Published : 2021.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The purpose of this animal research was to compare bone regeneration in augmented rabbit maxillary sinuses treated with demineralized particulate human-tooth graft and anorganic bovine bone by immunohistochemical analysis. Materials and Methods: Piezoelectric bilateral sinus augmentation was performed in eight adult rabbits. In the control group, anorganic bovine was grafted in the maxillary sinus following elevation of the sinus membrane. In the experimental group, demineralized human particulate tooth bone was grafted in the sinus. Bone regeneration in augmented sinuses was evaluated by immunohistochemical analysis using various markers of osteoprogenitor cells. Results: The number of bromodeoxyuridine-labeled cells was significantly higher in the experimental group than in the control group at eight weeks. The immunoreactivity of proliferating-cell nuclear antigen was increased slightly in the experimental group relative to the control group at eight weeks. Other bone markers were expressed equally in the two groups. Conclusion: In the rabbit maxillary sinus, higher osteoinduction was correlated with demineralized human particulate tooth bone grafting than with anorganic bovine grafting.

Keywords

References

  1. Misch CE. Maxillary sinus augmentation for endosteal implants: organized alternative treatment plans. Int J Oral Implantol 1987;4:49-58.
  2. Aghaloo TL, Moy PK. Which hard tissue augmentation techniques are the most successful in furnishing bony support for implant placement? Int J Oral Maxillofac Implants 2007;22 Suppl:49-70.
  3. Duan DH, Fu JH, Qi W, Du Y, Pan J, Wang HL. Graft-free maxillary sinus floor elevation: a systematic review and metaanalysis. J Periodontol 2017;88:550-64. https://doi.org/10.1902/jop.2017.160665
  4. Kent JN, Block MS. Simultaneous maxillary sinus floor bone grafting and placement of hydroxylapatite-coated implants. J Oral Maxillofac Surg 1989;47:238-42. https://doi.org/10.1016/0278-2391(89)90225-5
  5. Wagner JR. A 3 1/2-year clinical evaluation of resorbable hydroxylapatite OsteoGen (HA Resorb) used for sinus lift augmentations in conjunction with the insertion of endosseous implants. J Oral Implantol 1991;17:152-64.
  6. Cordioli G, Mazzocco C, Schepers E, Brugnolo E, Majzoub Z. Maxillary sinus floor augmentation using bioactive glass granules and autogenous bone with simultaneous implant placement. Clinical and histological findings. Clin Oral Implants Res 2001;12:270-8. https://doi.org/10.1034/j.1600-0501.2001.012003270.x
  7. Moon JW, Sohn DS, Heo JU, Kim JS. Comparison of two kinds of bovine bone in maxillary sinus augmentation: a histomorphometric study. Implant Dent 2015;24:19-24. https://doi.org/10.1097/ID.0000000000000187
  8. Lee KH, Kim YK, Cho WJ, Um IW, Murata M, Mitsugi M. Autogenous tooth bone graft block for sinus augmentation with simultaneous implant installation: a technical note. J Korean Assoc Oral Maxillofac Surg 2015;41:284-9. https://doi.org/10.5125/jkaoms.2015.41.5.284
  9. Boyne PJ, James RA. Grafting of the maxillary sinus floor with autogenous marrow and bone. J Oral Surg 1980;38:613-6.
  10. van den Bergh JP, ten Bruggenkate CM, Krekeler G, Tuinzing DB. Sinusfloor elevation and grafting with autogenous iliac crest bone. Clin Oral Implants Res 1998;9:429-35. https://doi.org/10.1034/j.1600-0501.1996.090608.x
  11. Yeomans JD, Urist MR. Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues. Arch Oral Biol 1967;12:999-1008. https://doi.org/10.1016/0003-9969(67)90095-7
  12. Bessho K, Tagawa T, Murata M. Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction. J Oral Maxillofac Surg 1990;48:162-9. https://doi.org/10.1016/s0278-2391(10)80204-6
  13. Jun SH, Ahn JS, Lee JI, Ahn KJ, Yun PY, Kim YK. A prospective study on the effectiveness of newly developed autogenous tooth bone graft material for sinus bone graft procedure. J Adv Prosthodont 2014;6:528-38. https://doi.org/10.4047/jap.2014.6.6.528
  14. Kim YK, Lee J, Yun JY, Yun PY, Um IW. Comparison of autogenous tooth bone graft and synthetic bone graft materials used for bone resorption around implants after crestal approach sinus lifting: a retrospective study. J Periodontal Implant Sci 2014;44:216-21. https://doi.org/10.5051/jpis.2014.44.5.216
  15. Kim ES. Autogenous fresh demineralized tooth graft prepared at chairside for dental implant. Maxillofac Plast Reconstr Surg 2015;37:8. https://doi.org/10.1186/s40902-015-0009-1
  16. Kim YK, Lee JH, Um IW, Cho WJ. Guided bone regeneration using demineralized dentin matrix: long-term follow-up. J Oral Maxillofac Surg 2016;74:515.e1-9. https://doi.org/10.1016/j.joms.2015.10.030
  17. Kim ES, Kang JY, Kim JJ, Kim KW, Lee EY. Space maintenance in autogenous fresh demineralized tooth blocks with platelet-rich plasma for maxillary sinus bone formation: a prospective study. Springerplus 2016;5:274. https://doi.org/10.1186/s40064-016-1886-1
  18. Lee J, Lee EY, Park EJ, Kim ES. An alternative treatment option for a bony defect from large odontoma using recycled demineralization at chairside. J Korean Assoc Oral Maxillofac Surg 2015;41:109-15. https://doi.org/10.5125/jkaoms.2015.41.2.109
  19. Kabir MA, Murata M, Akazawa T, Kusano K, Yamada K, Ito M. Evaluation of perforated demineralized dentin scaffold on bone regeneration in critical-size sheep iliac defects. Clin Oral Implants Res 2017;28:e227-35. https://doi.org/10.1111/clr.13000
  20. Xu X, Sohn DS, Kim HG, Lee SJ, Moon YS. Comparative histomorphometric analysis of maxillary sinus augmentation with deproteinized bovine bone and demineralized particulate human tooth graft: an experimental study in rabbits. Implant Dent 2018;27:324-31. https://doi.org/10.1097/ID.0000000000000755
  21. Ramos-Vara JA, Miller MA. When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique. Vet Pathol 2014;51:42-87. https://doi.org/10.1177/0300985813505879
  22. Dolbeare F. Bromodeoxyuridine: a diagnostic tool in biology and medicine, part I: historical perspectives, histochemical methods and cell kinetics. Histochem J 1995;27:339-69. https://doi.org/10.1007/BF02389022
  23. Okafuji N, Liu ZJ, King GJ. Assessment of cell proliferation during mandibular distraction osteogenesis in the maturing rat. Am J Orthod Dentofacial Orthop 2006;130:612-21. https://doi.org/10.1016/j.ajodo.2005.06.023
  24. Shimada A, Shibata T, Komatsu K, Nifuji A. Improved methods for immunohistochemical detection of BrdU in hard tissue. J Immunol Methods 2008;339:11-6. https://doi.org/10.1016/j.jim.2008.07.013
  25. Prelich G, Tan CK, Kostura M, Mathews MB, So AG, Downey KM, et al. Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein. Nature 1987;326:517-20. https://doi.org/10.1038/326517a0
  26. Garcia RL, Coltrera MD, Gown AM. Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis. Am J Pathol 1989;134:733-9.
  27. Mandir N, Englyst H, Goodlad RA. Resistant carbohydrates stimulate cell proliferation and crypt fission in wild-type mice and in the Apc(Min/+) mouse model of intestinal cancer, association with enhanced polyp development. Br J Nutr 2008;100:711-21. https://doi.org/10.1017/S0007114508901276
  28. Yasui N, Sato M, Ochi T, Kimura T, Kawahata H, Kitamura Y, et al. Three modes of ossification during distraction osteogenesis in the rat. J Bone Joint Surg Br 1997;79:824-30. https://doi.org/10.1302/0301-620x.79b5.7423
  29. Sodek J, Ganss B, McKee MD. Osteopontin. Crit Rev Oral Biol Med 2000;11:279-303. https://doi.org/10.1177/10454411000110030101
  30. Standal T, Borset M, Sundan A. Role of osteopontin in adhesion, migration, cell survival and bone remodeling. Exp Oncol 2004;26:179-84.
  31. Desbois C, Karsenty G. Osteocalcin cluster: implications for functional studies. J Cell Biochem 1995;57:379-83. https://doi.org/10.1002/jcb.240570302
  32. Sato M, Yasui N, Nakase T, Kawahata H, Sugimoto M, Hirota S, et al. Expression of bone matrix proteins mRNA during distraction osteogenesis. J Bone Miner Res 1998;13:1221-31. https://doi.org/10.1359/jbmr.1998.13.8.1221
  33. Sakamoto A, Oda Y, Iwamoto Y, Tsuneyoshi M. A comparative study of fibrous dysplasia and osteofibrous dysplasia with regard to expressions of c-fos and c-jun products and bone matrix proteins: a clinicopathologic review and immunohistochemical study of cfos, c-jun, type I collagen, osteonectin, osteopontin, and osteocalcin. Hum Pathol 1999;30:1418-26. https://doi.org/10.1016/s0046-8177(99)90162-4