Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Valeriana jatamansi Jones Inhibits Rotavirus-Induced Diarrhea via Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway

  • Zhang, Bin (Jiangsu Agri-Animal Husbandry Vocational College) ;
  • Wang, Yan (Food, Animal and Plant Inspection and Quarantine Technical Center of Shanghai Customs) ;
  • Jiang, Chunmao (Jiangsu Agri-Animal Husbandry Vocational College) ;
  • Wu, Caihong (Jiangsu Agri-Animal Husbandry Vocational College) ;
  • Guo, Guangfu (Jiangsu Agri-Animal Husbandry Vocational College) ;
  • Chen, Xiaolan (Jiangsu Agri-Animal Husbandry Vocational College) ;
  • Qiu, Shulei (Jiangsu Agri-Animal Husbandry Vocational College)
  • Received : 2020.03.05
  • Accepted : 2020.05.18
  • Published : 2021.08.28
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Abstract

Rotavirus (RV), as the main cause of diarrhea in children under 5 years, contributes to various childhood diseases. Valeriana jatamansi Jones is a traditional Chinese herb and possesses antiviral effects. In this study we investigated the potential mechanisms of V. jatamansi Jones in RV-induced diarrhea. MTT assay was performed to evaluate cell proliferation and the diarrhea mice model was constructed using SA11 infection. Mice were administered V. jatamansi Jones and ribavirin. Diarrhea score was used to evaluate the treatment effect. The enzyme-linked immunosorbent assay was performed to detect the level of cytokines. Western blot and quantitative reverse transcription-PCR were used to determine protein and mRNA levels, respectively. Hematoxylin-eosin staining was applied to detect the pathological change of the small intestine. TdT-mediated dUTP nick-end labeling was conducted to determine the apoptosis rate. The results showed V. jatamansi Jones promoted MA104 proliferation. V. jatamansi Jones downregulated phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT) in protein level, which was consistent with the immunohistochemistry results. Moreover, V. jatamansi Jones combined with ribavirin regulated interleukin-1β (IL-1β), interferon γ, IL-6, tumor necrosis factor α, and IL-10, and suppressed secretory immunoglobulin A secretion to remove viruses and inhibit dehydration. V. jatamansi Jones + ribavirin facilitated the apoptosis of small intestine cells. In conclusion, V. jatamansi Jones may inhibit RV-induced diarrhea through PI3K/AKT signaling pathway, and could therefore be a potential therapy for diarrhea.

Keywords

Acknowledgement

This study was suported by Jiangsu Province Agricultural Three New Projects (SXGC[2017]249), Jiangsu Province Agri-animal Husbandry Vocational Project (NSF201901), and Qing Lan Project of Jiangsu.

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