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A regression for estimating metabolizable glucose in diets of weaned piglets for optimal growth performance

  • Lv, Liangkang (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Feng, Zhi (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Zhang, Dandan (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Lei, Long (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Zhang, Hui (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Liu, Zhengya (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Ren, Ying (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University) ;
  • Zhao, Shengjun (Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University)
  • Received : 2020.07.02
  • Accepted : 2020.12.08
  • Published : 2021.10.01

Abstract

Objective: Two experiments were conducted to provide a new approach for evaluating feed nutritional value by metabolizable glucose (MG) in piglet diets with different levels of starch and crude fiber. In Exp 1, a regression equation for MG was generated. In Exp 2, the equation was verified, and the optimal growth performance of piglets under appropriate MG levels was tested. Methods: In Exp 1, 20 weaned piglets (7.74±0.81 kg body weight [BW]) were randomly assigned to 1 of 4 treatments, including the basal diet containing different levels of MG (starch, 25.80%, 31.67%, 45.71%, 49.36%; crude fiber, 1.23%, 1.35%, 1.80%, 1.51%). The piglets were implanted with an ileal fistula, cannulation of the carotid artery, portal vein, and mesenteric artery. The chyme from the ileum fistula and blood samples were collected. In Exp 2, 30 weaned piglets (8.96±0.50 kg BW) were randomly assigned to 1 of 5 treatments, including the experimental diets with different levels of MG (37.6, 132.5, 300.0, 354.3, and 412.5 g/kg). The piglets' BW, and feed consumption were recorded to calculate growth performance during the 28-d experiment. Results: In Exp 1, the MG levels in 4 diets were 239.62, 280.68, 400.79, and 454.35 g/kg. The regression equation for the MG levels and dietary nutrients was: Y (MG) = 12.13×X1 (starch)+23.18×X2 (crude fiber)-196.44 (R2 = 0.9989, p = 0.033). In Exp 2, treatments with 132.5 and 300.0 g/kg MG significantly (p<0.05) increased average daily gain and feed conversion efficiency of weaned piglets, increased digestibility of crude fat, and had no effect on digestibility of crude protein compared to 300.0 to 412.5 g/kg MG. Conclusion: The pig model combining the ileum fistula and cannulation of blood vessels was successfully used to determine the dietary MG levels. The recommended MG level in weaned pig diets is 132.5 to 300.0 g/kg.

Keywords

Acknowledgement

We wish to acknowledge the support of the technical staff of the Animal Care and Use Committee of Wuhan Polytechnic University in daily management. This work was financially supported by the National Natural Science Foundation of China (grant number 31201833 and 31872373) and the Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science (grant number 201904).

References

  1. Wang CH, Persyn A, Krackov J. Role of the krebs cycle in ethylene biosynthesis. Nature 1962;195:1306-8. https://doi.org/10.1038/1951306a0
  2. Saito T, Minakami S. Studies on erythrocyte glycolysis: VI. Control of glycolysis by ATP level in human erythrocytes. J Biochem 1967;61:211-9. https://doi.org/10.1093/oxfordjournals.jbchem.a128533
  3. Vente-Spreeuwenberg MAM, Verdonk JMAJ, Verstegen MWA, Beynen AC. Villus height and gut development in weaned piglets receiving diets containing either glucose, lactose or starch. Br J Nutr 2003;90:907-13. https://doi.org/10.1079/BJN2003981
  4. Zhao J, Bai Y, Zhang G, Liu L, Lai CH. Relationship between dietary fiber fermentation and volatile fatty acids' concentration in growing pigs. Animals 2020;10:263. https://doi.org/10.3390/ani10020263
  5. Bergman EN. Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiol Rev 1990;70:567-90. https://doi.org/10.1152/physrev.1990.70.2.567
  6. Lu DX. Theoretical system of glucose nutrition manipulation for ruminants and its application in feeding practice. Anim Husb Feed Sci 2010;31:402-9 [English abstract].
  7. Lu DX. Systems nutrition: an innovation of a scientific system in animal nutrition. Front Biosci 2014;E6:55-61. https://doi.org/10.2741/e690
  8. Sun J, Xu JH, Shen YZ, Wang MZ, Yu LH, Wang HR. Effects of different dietary ratio of physically effective neutral detergent fiber and metabolizable glucose on rumen fermentation, blood metabolites and growth performance of 8 to 10-monthold heifers. Asian-Australas J Anim Sci 2018;31:1230-7. https://doi.org/10.5713/ajas.17.0885
  9. Song LR, Xue B, Yan FQ, Yan TH, Xiao J. Dietary metabolizable glucose level affects performance and physiological indices of dairy cows under heat stress. Chinese J Anim Nutr 2014;26:1477-85 [English abstract].
  10. Fu C, Wang HR, Wang MZ, Yu LH, Huo YJ. Effects of different dietary metabolizable glucose levels on growth and development, nutrients digestibility and serum biochemical indices of 8 to 10-month-old heifers. Chinese J Anim Nutr 2014;26:2615-22 [English abstract]. https://doi.org/10.3969/j.issn.1006-267x.2014.09.022
  11. Kim HD, Mcmanus TJ. Studies on the energy metabolism of pig red cells: I. The limiting role of membrane permeability in glycolysis. Biochim Biophys Acta Gen Subj 1971;230:1-11. https://doi.org/10.1016/0304-4165(71)90048-1
  12. Kil DY, Kim BG, Stein HH. Feed Energy Evaluation for Growing Pigs. Asian-Australas J Anim Sci 2013;26:1205-17. https://doi.org/10.5713/ajas.2013.r.02
  13. Yen JT, Nienaber JA, Hill DA, Pond WG. Potential contribution of absorbed volatile fatty acids to whole-animal energy requirement in conscious swine. J Anim Sci 1991;69:2001-12. https://doi.org/10.2527/1991.6952001x
  14. Zhao SJ, Ren Y. Research development of new evaluating system of metabolizable nutrition in swine: a review. Chinese J Anim Nutr 2017;29:1455-61 [English abstract].
  15. NRC. Committee on Nutrient Requirements of Swine. Nutrient requirements of swine. 11th ed. Washington, DC, USA: National Academies Press; 2012.
  16. Jones RS, Yee TK, Michielsen CE. A modified Thomas Cannula for gastric and intestinal fistulas. J Appl Physiol 1971;30:427-8. https://doi.org/10.1152/jappl.1971.30.3.427
  17. Yen JT, Killefer J. A method for chronically quantifying net absorption of nutrients and gut metabolites into hepatic portal vein in conscious swine. J Anim Sci 1987;64:923-34. https://doi.org/10.2527/jas1987.643923x
  18. Fang ZF, Peng J, Qi ZL, Huang FR. Establishment of techniques for implanting arterial and portal catheters in piglets used to investigate intestinal nutrient metabolism. Chinese J Vet Sci 2010;30:1098-102 [English abstract].
  19. Katz ML, Bergman EN. Simultaneous measurements of hepatic and portal venous blood flow in the sheep and dog. Am J Physiol - Legacy Content 1969;216:946-52. https://doi.org/10.1152/ajplegacy.1969.216.4.946
  20. Mclean E, Ash R. Chronic cannulation of the hepatic portal vein in rainbow trout, Salmo gairdneri: A prerequisite to net absorption studies. Aquaculture 1989;78:195-205. https://doi.org/10.1016/0044-8486(89)90032-X
  21. Mccleary BV, Gibson TS, Solah VA, Mugford DC. Total starch measurement in cereal products: Interlaboratory evaluation of a rapid enzymic test procedure. Cereal Chem 1994;71:501-5.
  22. Titgemeyer EC, Armendariz CK, Bindel DJ, Greenwood RH, Loest CA. Evaluation of titanium dioxide as a digestibility marker for cattle. J Anim Sci 2001;79:1059-63. https://doi.org/10.2527/2001.7941059x
  23. Anderson DM. Proceedings: The measurement of portal and hepatic blood flow in pigs. Proc Nutr Soc 1974;33:30A-1A.
  24. Ven der Meulen J, Bakker JGM, Smits B, Visser HD. Effect of source of starch on net portal flux of glucose, lactate, volatile fatty acids and amino acids in the pig. Br J Nutr 1997;78:533-44. https://doi.org/10.1079/bjn19970173
  25. Wolin MJ. Fermentation in the rumen and human large intestine. Science 1981;213:1463-8. https://doi.org/10.1126/science.7280665
  26. Yen JT, Nienaber JA, Hill DA, Pond WG. Potential contribution of absorbed volatile fatty acids to whole-animal energy requirement in conscious swine. J Anim Sci 1991;69:2001-12. https://doi.org/10.2527/1991.6952001x
  27. Zhang S, Yoo DH, Ao X, Kim IH. Effects of dietary probiotic, liquid feed and nutritional concentration on the growth performance, nutrient digestibility and fecal score of weaning piglets. Asian-Australas J Anim Sci 2020;33:1617-23. https://doi.org/10.5713/ajas.19.0473
  28. Caine WR, Sauer WC, Tamminga S, Verstegen MWA, Schulze H. Apparent ileal digestibilities of amino acids in newly weaned pigs fed diets with protease-treated soybean meal. J Anim Sci 1997;75:2962-9. https://doi.org/10.2527/1997.75112962x