Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows

  • Li, Han (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Wang, Xiangming (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Wu, Yan (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Zhang, Dingran (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xu, Hongyang (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xu, Hongrun (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xing, Xiaoguang (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Qi, Zhili (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University)
  • Received : 2020.08.11
  • Accepted : 2020.10.27
  • Published : 2021.09.01
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Abstract

Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.

Keywords

Acknowledgement

This work was supported by the National Key Research and Development Program of China (grant number 2018YFD0501605); the Fundamental Research Funds for the Central Universities (grant number 2662018Y079); and the Hubei Key Laboratory of Animal Nutrition (grant number 2004DA125184F1721).

References

  1. Randall LV, Green MJ, Chagunda MGG, Mason C, Green LE, Huxley JN. Lameness in dairy heifers; impacts of hoof lesions present around first calving on future lameness, milk yield and culling risk. Prev Vet Med 2016;133:52-63. https://doi.org/10.1016/j.prevetmed.2016.09.006
  2. Bay V, Griffiths B, Carter S, et al. 16S rRNA amplicon sequencing reveals a polymicrobial nature of complicated claw horn disruption lesions and interdigital phlegmon in dairy cattle. Sci Rep 2018;8:15529. https://doi.org/10.1038/s41598-018-33993-9
  3. Miguel-Pacheco GG, Thomas HJ, Huxley JN, Newsome RF, Kaler J. Effect of claw horn lesion type and severity at the time of treatment on outcome of lameness in dairy cows. Vet J 2017;225:16-22. https://doi.org/10.1016/j.tvjl.2017.04.015
  4. Cook NB, Hess JP, Foy MR, Bennett TB, Brotzman RL. Management characteristics, lameness, and body injuries of dairy cattle housed in high-performance dairy herds in Wisconsin. J Dairy Sci 2016;99:5879-91. https://doi.org/10.3168/jds.2016-10956
  5. Cole KJ, Hogan JS. Short communication: environmental mastitis pathogen counts in freestalls bedded with composted and fresh recycled manure solids. J Dairy Sci 2016;99:1501-5. https://doi.org/10.3168/jds.2015-10238
  6. Oehme B, Geiger SM, Grund S, Hainke K, Munzel J, Mulling CKW. Effect of different flooring types on pressure distribution under the bovine claw-an ex vivo study. BMC Vet Res 2018;14:259. https://doi.org/10.1186/s12917-018-1579-9
  7. Rowbotham RF, Ruegg PL. Association of bedding types with management practices and indicators of milk quality on larger Wisconsin dairy farms. J Dairy Sci 2015;98:7865-85. https://doi.org/10.3168/jds.2015-9866
  8. Burgstaller J, Raith J, Kuchling S, Mandl V, Hund A, Kofler J. Claw health and prevalence of lameness in cows from compost bedded and cubicle freestall dairy barns in Austria. Vet J 2016;216:81-6. https://doi.org/10.1016/j.tvjl.2016.07.006
  9. Evans NJ, Timofte D, Isherwood DR, et al. Host and environmental reservoirs of infection for bovine digital dermatitis treponemes. Vet Microbiol 2012;156:102-9. https://doi.org/10.1016/j.vetmic.2011.09.029
  10. Zinicola M, Lima F, Lima S, et al. Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir. PLoS One 2015;10:e0120504. https://doi.org/10.1371/journal.pone.0120504
  11. Ito K, von Keyserlingk MAG, LeBlanc SJ, Weary DM. Lying behavior as an indicator of lameness in dairy cows. J Dairy Sci 2010;93:3553-60. https://doi.org/10.3168/jds.2009-2951
  12. Krull AC, Shearer JK, Gorden PJ, Cooper VL, Phillips GJ, Plummer PJ. Deep sequencing analysis reveals temporal microbiota changes associated with development of bovine digital dermatitis. Infect Immun 2014;82:3359-73. https://doi.org/10.1128/IAI.02077-14
  13. Nielsen MW, Strube ML, Isbrand A, et al. Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples. Vet Microbiol 2016;186:139-49. https://doi.org/10.1016/j.vetmic.2016.03.003
  14. Klitgaard K, Nielsen MW, Ingerslev HC, Boye M, Jensen TK. Discovery of bovine digital dermatitis-associated Treponema spp. in the dairy herd environment by a targeted deep-sequencing approach. Appl Environ Microbiol 2014;80:4427-32. https://doi.org/10.1128/AEM.00873-14
  15. Klitgaard K, Strube ML, Isbrand A, Jensen TK, Nielsen MW. Microbiota analysis of an environmental slurry and its potential role as a reservoir of bovine digital dermatitis pathogens. Appl Environ Microbiol 2017;83:e00244-17. https://doi.org/10.1128/AEM.00244-17
  16. Norring M, Manninen E, De Passille AM, Rushen J, Munksggard L, Saloniemi H. Effects of sand and straw bedding on the lying behavior, cleanliness, and hoof and hock injuries of dairy cows. J Dairy Sci 2008;91:570-6. https://doi.org/10.3168/jds.2007-0452
  17. Flower FC, Weary DM. Effect of hoof pathologies on subjective assessments of dairy cow gait. J Dairy Sci 2006;89:139-46. https://doi.org/10.3168/jds.S0022-0302(06)72077-X
  18. Landewe R, Geusens P, Boers M, et al. Markers for type II collagen breakdown predict the effect of disease-modifying treatment on long-term radiographic progression in patients with rheumatoid arthritis. Arthritis Rheum 2004;50:1390-9. https://doi.org/10.1002/art.20222
  19. Skioldebrand E, Ekman S, Mattsson Hulten L, et al. Cartilage oligomeric matrix protein neoepitope in the synovial fluid of horses with acute lameness: a new biomarker for the early stages of osteoarthritis. Equine Vet J 2017;49:662-7. https://doi.org/10.1111/evj.12666
  20. Garnero P, Ayral X, Rousseau JC, et al. Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritis. Arthritis Rheum 2002;46:2613-24. https://doi.org/10.1002/art.10576
  21. Zhao XJ, Wang XY, Wang JH, Wang ZY, Wang L, Wang ZH. Oxidative stress and imbalance of mineral metabolism contribute to lameness in dairy cows. Biol Trace Elem Res 2015; 164:43-9. https://doi.org/10.1007/s12011-014-0207-1
  22. Liu J, Zhang M, Zhang R, Zhu W, Mao S. Comparative studies of the composition of bacterial microbiota associated with the ruminal content, ruminal epithelium and in the faeces of lactating dairy cows. Microb Biotechnol 2016;9:257-68. https://doi.org/10.1111/1751-7915.12345
  23. Li J, Rui J, Yao M, et al. Substrate type and free ammonia determine bacterial community structure in full-scale mesophilic anaerobic digesters treating cattle or swine manure. Front Microbiol 2015;6:1337. https://doi.org/10.3389/fmicb.2015.01337
  24. Ming L, Yi L, Siriguleng, et al. Comparative analysis of fecal microbial communities in cattle and Bactrian camels. PLoS One 2017;12:e0173062. https://doi.org/10.1371/journal.pone.0173062
  25. Han Z, Zeng D, Mou Z, Shi G, Zhang Y, Lou Z. A novel spatiotemporally anaerobic/semi-aerobic bioreactor for domestic solid waste treatment in rural areas. Waste Manage 2019;86:97-105. https://doi.org/10.1016/j.wasman.2019.01.034
  26. Maboni G, Blanchard A, Frosth S, Stewart C, Emes R, Totemeyer S. A distinct bacterial dysbiosis associated skin inflammation in ovine footrot. Sci Rep 2017;7:45220. https://doi.org/10.1038/srep45220
  27. Ridaura VK, Bouladoux N, Claesen J, et al. Contextual control of skin immunity and inflammation by Corynebacterium. J Exp Med 2018;215:785-99. https://doi.org/10.1084/jem.20171079
  28. Kim JY, Kim YB, Song HS, et al. Genomic analysis of a pathogenic bacterium, Paeniclostridium sordellii CBA7122 containing the highest number of rRNA operons, isolated from a human stool sample. Front Pharmacol 2017;8:840. https://doi.org/10.3389/fphar.2017.00840
  29. Corona PS, Haddad S, Andres J, Gonzalez-Lopez JJ, Amat C, Flores X. Case report: first report of a prosthetic joint infection caused by Facklamia hominis. Diagn Microbiol Infect Dis 2014;80:338-40. https://doi.org/10.1016/j.diagmicrobio.2014.08.008
  30. Lu Y, Chen J, Zheng J, et al. Mucosal adherent bacterial dysbiosis in patients with colorectal adenomas. Sci Rep 2016;6:26337. https://doi.org/10.1038/srep26337

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