Current Optics and Photonics

Optical Society of Korea (한국광학회)
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Enhancement of Speckle Contrast in vivo by Combining Linearly Polarized Laser Light and an Analyzer

  • Qureshi, Muhammad Mohsin (Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Mac, Khuong Duy (Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Kim, Andrew Hyunjin (Department of Mechanical Engineering, The State University of New York Korea) ;
  • Kim, Young Ro (Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital) ;
  • Chung, Euiheon (Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology)
  • Received : 2021.03.05
  • Accepted : 2021.04.21
  • Published : 2021.08.25
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Abstract

Speckle imaging is capable of dynamic data acquisition at high spatiotemporal resolution, and has played a vital role in the functional study of biological specimens. The presence of various optical scatterers within the tissue causes alteration of speckle contrast. Thus structures like blood vessels can be delineated and quantified. Although laser speckle imaging is frequently used, an optimization process to ensure the maximum speckle contrast has not been available. In this respect, we here report an experimental procedure to optimize speckle contrast via applying different combinations of varying polarization of the illuminating laser light and multiple analyzer angles. Specifically, samples were illuminated by the p-polarization, 45°-polarization, and s-polarization of the incident laser, and speckle images were recorded without and with the analyzer rotated from 0° to 180° (Δ = 30°). Following the baseline imaging of a solid diffuser and a fixed brain sample, laser speckle contrast imaging (LSCI) was successfully performed to visualize in vivo mouse-brain blood flow. For oblique laser illumination, the maximum contrast achieved with p-polarized and s-polarized light was perpendicular to the analyzer's axis. This study demonstrates the optimization process for maximizing the speckle contrast, which can improve blood-flow estimation in vivo.

Keywords

Acknowledgement

This work was supported by the GIST Research Institute (GRI) GIST-CNUH research collaboration grant, funded by GIST in 2021 and the Joint Research Project of the Institutes of Science and Technology in 2021. This work was supported by the Institute of Information & Communications Technology Planning & Evaluation (IITP) grant, funded by the Korea government (MSIT) (No.2019-0-01842, Artificial Intelligence Graduate School Program (GIST)). This research was also supported by the National Research Foundation of Korea (NRF), funded by the Korean government (MEST) (NRF-2019R1A2C2086003), and the Brain Research Program through the NRF, funded by the Ministry of Science, ICT & Future Planning (NRF-2017M3C7A1044964), and finally by the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, Republic of Korea, and the Ministry of Food and Drug Safety (Project Number: 202011D13).

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