The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Effect of Geumeunwha-san Water Extract on LPS-induced Raw 264.7 Cells

금은화산(金銀花散)의 LPS로 유도된 Raw 264.7 세포에서의 항염증 효과

  • Kim, Yeon-Soo (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Kim, Su-Jin (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Jee, Seon-Young (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Hwangbo, Min (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
  • 김연수 (대구한의대 부속 대구한방병원 안이비인후피부과) ;
  • 김수진 (대구한의대 부속 대구한방병원 안이비인후피부과) ;
  • 지선영 (대구한의대 부속 포항한방병원 안이비인후피부과) ;
  • 황보민 (대구한의대 부속 대구한방병원 안이비인후피부과)
  • Received : 2021.06.25
  • Accepted : 2021.07.31
  • Published : 2021.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of this study is to investigate the anti-inflammatory effect of Geumeunwha-san(GEHS) water extract in vitro. Methods : To evaluate the anti-inflammatory effect of GEHS, Raw 264.7 cells were pretreated with 10-300㎍/㎖ of GEHS for 1hr, and then exposed to 1㎍/㎖ of LPS. MTT assay was used to detect the cell viability. Productions of pro-inflammatory cytokines and NO were measured in culture media. By using immunoblot analysis, protein levels of iNOS and NF-𝜅B were determined. Results : In vitro study, cell viability assay on GEHS treatment of 10-300㎍/㎖ has no cytotoxicity in Raw 264.7 cells. Pretreated 100, 300㎍/㎖ of GEHS had significantly inhibited LPS-induced NO production. And also pretreatment of 100, 300㎍/㎖ GEHS had significantly decreased production of interleukin-6, -1𝛽 and tumor necrosis factor-𝛼 in LPS-activated Raw 264.7 cells. In addition, GEHS reduced LPS-mediated iNOS expression. Moreover I-𝜅B𝛼 expression was significantly induced by GEHS and NF-𝜅B expression was reduced by GEHS. Conclusions : These results suggest the clinical basis of GEHS for the treatment of inflammatory diseases.

Keywords

References

  1. Jeon DH. Silyongjunguidaeyagbang. Beijing: People's Medical Publishing House. 2000:718.
  2. Kim Y, Yang SY, Oh YS, Lee JW, Lee YK, Park YC. Research trends of lonicera japonica over the last 10 year. J Daejeon Orient Med. 2010;19(1):17-23.
  3. Joo JS, Kim JS, Jeong JG, Kim BK. Study of efficacy of Foeniculi Fructus & Lonicerae Flos extract on acute pancreatitis. Kor J Herbology. 2010;25(4):39-45. https://doi.org/10.6116/KJH.2010.25.4.039
  4. Bak JP, Son JH, Kim YM, Lee EY, Leem KH, Kim EH. Suppression of inflammatory macrophage response by Glycyrrhiza Uralensis herbal acupuncture extract. Korean J Acupunct. 2011;28(4):49-58.
  5. Heo J. Donguibogam. 1st ed. Seoul:Beopinmunwhasa. 1994:683,684,686,1542,1545.
  6. Park CH, Kim JH, Choi SH, Shin YS, Lee SW, Cho EJ. Protective effects of Glycyrrhiza Uralensis Radix extract and its active compounds on H2O2-induced apoptosis of C6 glial cells. Korean J Med Crop Sci. 2017;25(5):315-21. https://doi.org/10.7783/KJMCS.2017.25.5.315
  7. Cha HY, Jeong AR, Cheon JH, Ahn SH, Park SY, Kim KB. The Anti-oxidative and Anti-inflammatory effect of Lonicera Japonica on ulcerative colitis induced by Dextran Sulfate Sodium in mice. J Pediatr Korean Med. 2015;29(3):54-64. https://doi.org/10.7778/jpkm.2015.29.3.054
  8. Lee JH, Ko WS, Kim YH, Kang HS, Kim HD, Choi BT. Anti-inflammatory effect of the aqueous extract from lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver. Int J Mol Med. 2001;7:79-83.
  9. Yun YG, Kim GM, Lee SJ, Ryu SH, Jang SI. Inhibitory effect of aqueous extract from lonicera japonica flower on LPS-induced inflammatory mediators in RAW 264.7 Macrophages. Kor J Herbology. 2007;22 (3):117-25.
  10. Lee DE, Lee JR, Kim YW, Kwon YK, Byun SH, Shin SW, et al. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α, IL-1β and COX-2 expression by flower and whole plant of lonicera japonica. Korean J Oriental Physiology & Pathology. 2005;19(2):481-9.
  11. Kim SS, Shin JY, Park YM, Chung KM, Lee JH, Kweon DH. Investigation of antimicrobial activity and stability of ethanol extracts of licorice root (glycyrrhiza glabra). Korean J Food Sci Technol. 2006;38(2): 241-8.
  12. Lee KH, Rhee KH. Anti-inflammatory effects of glycyrrhiza glabra linne extract in a dextran sulfate sodium-induced colitis mouse model. Korean J Food & Nutr. 2010;23(4):435-9.
  13. Yoon TS, Cheon MS, Kim SJ, Lee AY, Moon BC, Chun JM, et al. Evaluation of solvent extraction on the anti-inflammatory efficacy of glycyrrhiza uralensis. Korean J Medicinal Crop Sci. 2010;18(1):28-33.
  14. Lee KW, Kim JI, Lee SY, Choi KM, Oh YT, Jeong JW. Induction of apoptosis by water extract of glycyrrhizae radix in human bladder T24 cancer cells. Korean J Plant Res. 2019;32(4):255-63.
  15. Lee YW, Ahn SH, Kim HH, Kim KB. The anti-inflammatory effect of lonicera japonica-glycyrrhiza uralensis decoction on ulcerative colitis induced by DSS in mice. J Pediatr Korean Med. 2018;32(3):16-25. https://doi.org/10.7778/JPKM.2018.32.3.0016
  16. Hayden MS, Ghosh S. NF-κB, the first-quarter-century: remarkable progress and outstanding questions. Genes Dev. 2012; 26(3):203-34. https://doi.org/10.1101/gad.183434.111
  17. Murray PJ, Wynn TA. Protective and pathogenic functions of macrophage subsets. Nat Rev Immunol. 2011;11(11):723-37. https://doi.org/10.1038/nri3073
  18. Kim NE, Kim YS, Jee SY, Hwangbo M. Anti-inflammatory effects of cheongsimyanggyeok-san via NF-κB inhibition. J Korean Med Opthalmol Otolaryngol Dermatol. 2019;32(2):11-23.
  19. Keum SY, Park SM, Jegal KH, Hwangbo M, Cho IJ, Park CA, et al. Anti-inflammatory effects of illicium verum hook. f. via suppression of NF-κB pathway. Herbal Formula Science. 2016;24(4):243-57. https://doi.org/10.14374/HFS.2016.24.4.243
  20. Abbas AK, Lichman AH. Cellular and molecular immunology. 5th ed. Philadelphia:Saunders. 2003:25,243-55,282-8,493, 507.
  21. Ko WS, Park HJ, Yoon HJ, Yoon JW, Yoon SW. Inhibitory effect of omisodok-eum on the secretion of NO in LPS-stimulated mouse peritoneal macrophages. Korean J Oriental Physiology & Pathology. 2002;16 (5):921-7.
  22. Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κBα phosphorylation, C/EBP and AP-1 activation. British journal of pharmacology. 2003; 139:11-20. https://doi.org/10.1038/sj.bjp.0705231
  23. Sohn KH, Kim SH. Antitumor activity of bupleuri radix and artemisiae capillaris herba and synergistic effect with anticancer Drugs. KOMS. 1995;16(2):414-32.
  24. Akira S, Uematsu S, Takeuchi O. Pathogen Recognition and innate immunity. Cell. 2006;124(4):783-801. https://doi.org/10.1016/j.cell.2006.02.015
  25. Judith AO, Jenni P, Sharon AS. Kuby Immunology. 7th ed. Seoul:Panmuneducation. 2014:133-71.
  26. Jiang YG. 50 Cases of laryngeal disease with cheongsimyanggyeok-sangamibang. Journal of Sichuan Traditional Chinese Medicine. 2011;19(11):65. https://doi.org/10.3969/j.issn.1000-3649.2001.11.053