Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Resveratrol and Aspartic acid Contents and Antiadipogenic Effect of Peanut and Peanut Sprout Extracts

땅콩과 땅콩새싹 추출물의 resveratrol과 aspartic acid 함량분석 및 지방세포분화 억제효능

  • Received : 2021.02.17
  • Accepted : 2021.04.13
  • Published : 2021.08.01
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Abstract

Interest in peanuts driven by their various biological activities and abundant nutrition engenders research interest in peanut sprouts for determining their biological activities and nutrition. Several research groups have recently studied the peanut sprout ingredients, but these studies focused on the peanut sprout extracted by methanol, an inedible extraction solvent. Thus, the present study provides the contents of two biologically active compounds, resveratrol and aspartic acid, in peanut and peanut sprout extracted by hot water and fermented ethanol, two edible extraction solvents, using UPLC-MS/MS technique. The UPLC-MS/MS results show that the peanut sprout extracted by fermented ethanol has the highest resveratrol and aspartic acid contents. This extract also exhibited the highest inhibitory effect on adipocyte differentiation, which the resveratrol may induce. Consequently, peanut sprout extracted by fermented ethanol might be helpful for the development of functional food plant materials.

식품산업 활용성을 고려하여 땅콩과 땅콩새싹의 열수와 주정 추출물을 제조하고 추출물내 resveratrol과 aspartic acid 함량을 UPLC-MS/MS를 이용하여 분석한 결과 땅콩새싹의 주정 추출물이 가장 높은 resveratrol과 aspartic acid 함량을 나타내었다. 또한 땅콩새싹의 주정 추출물은 3T3-L1 세포에 처리시 지방세포분화를 유의적으로 억제하였다. 따라서 땅콩새싹의 주정추출물은 기능성식품소재로 활용 가능성이 높을 것으로 판단된다. 향후 이러한 결과를 바탕으로 땅콩의 발아조건 및 추출 시간과 온도에 따른 활성성분 함량을 측정하여 최적의 활성성분 추출조건을 확립하는 연구를 진행할 계획이다.

Keywords

Acknowledgement

본 연구는 한국식품연구원 주요사업(E0210300-01) 지원에 의해 이루어졌습니다.

References

  1. Adhikari, B., S.K. Dhungana, M.W. Ali, A. Adhikari, I.D. Kim and D.H. Shin. 2018. Resveratrol, total phenolic and flavonoid contents, and antioxidant potential of seeds and sprouts of Korean peanuts. Food Sci. Biotechnol. 27:1275-1284. https://doi.org/10.1007/s10068-018-0364-7
  2. Bonnefont-Rousselot, D. 2016. Resveratrol and Cardiovascular Diseases. Nutrients 8:250. https://doi.org/10.3390/nu8050250
  3. Chen, S., Z. Li, W. Li, Z. Shan and W. Zhu. 2011. Resveratrol inhibits cell differentiation in 3T3-L1 adipocytes via activation of AMPK. Can. J. Physiol. Pharmacol. 89:793-799. https://doi.org/10.1139/y11-077
  4. Chiou, R.Y.Y., K.L. Ku and W.L. Chen. 1997. Compositional characterization of peanut kernels after subjection to various germination times. J. Agric. Food Chem. 45:3060-3064. https://doi.org/10.1021/jf970082j
  5. Chukwumah, Y.C., L.T. Walker, B. Vogler and M. Verghese. 2012. Profiling of bioactive compounds in cultivars of Runner and Valencia peanut market-types using liquid chromatography/APCI mass spectrometry. Food Chem. 132:525-531. https://doi.org/10.1016/j.foodchem.2011.10.050
  6. Chukwumah, Y.C., L.T. Walker, M. Verghese, M. Bokanga, S. Ogutu and K. Alphonse. 2007. Comparison of extraction methods for the quantification of selected phytochemicals in peanuts (Arachis hypogaea). J. Agric. Food Chem. 55:285-290. https://doi.org/10.1021/jf062148t
  7. Ha, A.W., W.K. Kim, J.H. Kim and N.E. Kang . 2015. The supplementation effects of peanut sprout on reduction of abdominal fat and health indices in overweight and obese women. Nutr. Res. Pract. 9:249-255. https://doi.org/10.4162/nrp.2015.9.3.249
  8. Kang, H.I., J.Y. Kim, K.W. Park, J.S. Kang, M.R. Choi, K.D. Moon and K.I. Seo. 2010. Resveratrol content and nutritional components in peanut sprouts. Korean J. Food Preserv. 17: 384-390.
  9. Kang, H.I., J.Y. Kim, S.J. Kwon, K.W. Park, J.S. Kang and K.I. Seo. 2010. Antioxidative effects of peanut sprout extracts. J. Korean Soc. Food Sci. Nutr. 39:941-946. https://doi.org/10.3746/jkfn.2010.39.7.941
  10. Kang , N.E., A.W. Ae Wha Ha, H.W. Woo and W.K. Kim. 2014. Peanut sprouts extract (Arachis hypogaea L.) has antiobesity effects by controlling the protein expressions of PPARγ and adiponectin of adipose tissue in rats fed high-fat diet. Nutr. Res. Pract. 8:158-164. https://doi.org/10.4162/nrp.2014.8.2.158
  11. Kim, S.H. and D.E. Jin. 2016. Effect of hangover drink using peanut (Arachis hypogaea) sprout extract on antioxidant and alcohol-degrading enzyme activity. J. Agric. Life Sci. 50: 223-231. https://doi.org/10.14397/jals.2016.50.1.223
  12. Kim, W.K., N.E. Kang, M.H. Kim and A.W. Ha. 2013. Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes. Nutr. Res. Pract. 7:160-165. https://doi.org/10.4162/nrp.2013.7.3.160
  13. Lastra, C.A. and I. Villegas. 2007. Resveratrol as an antioxidant and pro-oxidant agent: mechanisms and clinical implications. Biochem. Soc. Trans. 35:1156-1160. https://doi.org/10.1042/BST0351156
  14. Lee, M.J., Y.K. Cheong, H.S. Kim, K.H. Park, H.S. Doo and D.Y. Suh. 2003. Trans-resveratrol content of varieties and growth period in peanut. J. Korean Crop. Sci. 48:429-433.
  15. Lee, Y.M., E. Son and D.S. Kim. 2019. Treatment with peanut sprout root extract alleviates inflammation in a lipopolysaccharide-stimulated mouse macrophage cell line by inhibiting the MAPK signaling pathway. Int. J. Mol. Sci. 20:5907. https://doi.org/10.3390/ijms20235907
  16. Lim, S.H., S.H. Kim, J.J. Park, Y.S. Park, S.K. Dhungana, I.D. Kim and D.H. Shin. 2020. Quality characteristics and antioxidant activities of lotus (Nelumbo nucifera Gaertn.) sprouts grown under different conditions. Korean J. Plant Res. 33: 666-674. https://doi.org/10.7732/KJPR.2020.33.6.666
  17. Lopez, M.S., R.J. Dempsey and R. Vemuganti. 2015. Resveratrol neuroprotection in stroke and traumatic CNS injury. Neurochem. Int. 89:75-82. https://doi.org/10.1016/j.neuint.2015.08.009
  18. Park, C.H. and H.W. Park. 2002. Review of the studies on the qualities in peanut. Korean J. Crop. Sci. 47:163-174.
  19. Rauf, A., M. Imran, M.S. Butt, M. Nadeem, D.G. Peters and M.S. Mubarak. 2018. Resveratrol as an anti-cancer agent: A review. Crit. Rev. Food Sci. Nutr. 58:1428-1447. https://doi.org/10.1080/10408398.2016.1263597
  20. Seo, J.Y., S.S. Kim, H.J. Kim, K.H. Liu, H.Y. Lee and J.S. Kim. 2013. Laxative effect of peanut sprout extract. Nutr. Res. Pract. 7:262-266. https://doi.org/10.4162/nrp.2013.7.4.262
  21. Springer, M. and S. Moco. 2019. Resveratrol and its human metabolites-Effects on metabolic health and obesity. Nutrients 11:143. https://doi.org/10.3390/nu11010143
  22. Wang, K.H., Y.H. Lai, J.C. Chang, T.F. Ko, S.L. Shyu and R.Y.Y., Chiou, 2005. Germination of peanut kernels to enhance resveratrol biosynthesis and prepare sprouts as a functional vegetable. J. Agric. Food Chem. 53:242-246. https://doi.org/10.1021/jf048804b
  23. Yoon, M.Y. 2016. A study on peanut spouts extract as the anti-oxidant activity and the skin whitening cosmetic ingredients. KSBB J. 31:14-19. https://doi.org/10.7841/ksbbj.2016.31.1.14
  24. Zhang, G., Y. Liu, L. Xu, C. Sha, H. Zhang and W. Xu. 2019. Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model. BMC Biotechnol. 19:10. https://doi.org/10.1186/s12896-019-0502-1