The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant activity and NO production of the Alisma orientale Juzep fermented by Paenibacillus kribbensis AM49

Paenibacillus kribbensis AM49로 발효시킨 택사의 항산화 활성 및 NO 생성 효과

  • 유동진 (구운식품 기업부설 연구소) ;
  • 김창은 (구운식품 기업부설 연구소) ;
  • 유수정 (구운식품 기업부설 연구소) ;
  • 전문희 (구운식품 기업부설 연구소) ;
  • 김수현 (구운식품 기업부설 연구소)
  • Received : 2021.06.18
  • Accepted : 2021.07.25
  • Published : 2021.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of this study was to investigate the antioxidant activities and nitric oxide (NO) production in RAW 264.7 macrophages in extract of Alisma orientale Juzep (EAOJ) and fermented extract (FAOJ) by Paenibacillus kribbensis AM49 (P. kribbensis AM49). Methods : The Alisma orientale Juzep was fermented with P. kribbensis AM49 at 37℃ for 72 hours. We measured total polyphenol and total flavonoid, DPPH radical scavenging activity, FRAP activity and reducing power by spectrometric assay in EAOJ and FAOJ at concentrations at 0.5, 1, 5, 10 mg/㎖. Positive control was used ascorbic acid. Furthermore, we examined effect of EAOJ and FAOJ on the cell viability and NO production in RAW 264.7 macrophages. Results : The total polyphenol and total flavonoids content of FAOJ were increased 9.16 mg/g, 2.59 mg/g to 12.58 mg/g, 3.45 mg/g. DPPH radical scavenging activity, FRAP activity and reducing power were dose dependently increased according to the treatment concentration (0.5, 1, 5, 10 mg/㎖) of EAOJ and FAOJ. In particular, DPPH radical scavenging activity, FRAP activity of FAOJ was significantly increased at 5, 10 mg/㎖. Reducing power of FAOJ at 10 mg/㎖ was similar to ascorbic acid at 0.1 mg/㎖. In addition, the cell viability and NO production in RAW 264.7 macrophages were significantly increased at the concentrations of 250, 500, 1000 ㎍/㎖. Conclusions : These results suggest that FAOJ by P. kribbensis AM49 has effects to antioxidant activity. In addition, the cell viability and NO production in RAW 264.7 macrophages were significantly increased.

Keywords

References

  1. Pahng SH. A literature study on fermented korean medicinals used in dermatology. Korean J. Orient. Med. 2011 ; 17(3) : 53-60.
  2. Kong BM, Min JW, Kim SY. Physico-chemical characteristics of white, fermented and red ginseng extracts. J. Ginseng Res. 2008 ; 32(3) : 238-243. https://doi.org/10.5142/JGR.2008.32.3.238
  3. Jung YJ, Han DO, Park C, Lee HJ, Kim SH, Hahm DH. Effect of fermented herbal extracts, HP-1 on enzyme activities and gene expressions related to alcohol metabolism in ethanol-loaded rats. Korean J. Orient Physiology & Pathology. 2007 ; 21(2) : 387-391.
  4. Kang DH, Kim HS. Functionality analysis of korean medicine fermented by Lactobacillus strains. Korean J. Microbio. Biotechnol. 2011 ; 39(3) : 259-265.
  5. Choi, HJ, Kim DW, Joo, WH. Characteristics of Paenibacillus sp. BCNU 5016 as a novel probiotic. J. Life Sci. 2014 ; 24(2) : 161-166. https://doi.org/10.5352/JLS.2014.24.2.161
  6. Kim, YE. A study on Paeniacillus strains as probiotics. Changwon National University. Master's degree. 2011.
  7. Park JC, Hur JM, Kim SE. Isolation and quantitative analysis of alisol B 23-acetate from the rhizome of Alisma orientale. Korean J. Food & Nutr. 2005 ; 34(2) : 243-246. https://doi.org/10.3746/jkfn.2005.34.2.243
  8. Koo BH. Dictionary donguibogam. Seoul : Korea Dictionary Research Publishino. 2001 : 1406.
  9. Kim HG, Kim HM, Song BK, LEE EJ, Jung HT. Pharmacology of medicinal herbs. Seoul : Korea Medical Book Publishing Company. 2000 : 116.
  10. Chang IM, Kim YS, Yun HS, Kim SO. Liver-protective activities of alisol compounds against CCl4 intoxication. Kor. J. Pharmacog. 1982 ; 13(3) :112-115.
  11. IMAI Y, MATSUMURA H, ARAMAKI Y. Hypocholesterolemic Effect of alisol a-24-mono-acetate and its related compounds in rats. Jap. J. Pharmac. 1970 ; 20(2) : 222-228. https://doi.org/10.1254/jjp.20.222
  12. Kubo M, Matsuda H, Tomohiro N, Yoshikawa M. Studies on Alismatis Rhizoma. I. Anti-allergic effects of methanol extract and six terpene components from Alismatis Rhizoma (dried Rhizome of Alisma orientale). Biol Pharm Bull. 1997 ; 20(5) : 511-516. https://doi.org/10.1248/bpb.20.511
  13. Folin O, Denis W. Colorimetric method for the determination of phenols(phenol derivatives) in urine. J. Biol. Chem. 1915 ; 22(2) : 305-308. https://doi.org/10.1016/S0021-9258(18)87648-7
  14. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 2000 ; 71(1) : 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  15. Choi JS, Lee JH, Park HJ, Kim HG, Young HS, Mun SI. Screening for antioxidant activity of plants and marine algae and its active principles from Prunus davidiana. Kor. J. Pharmacog. 1993 ; 24(4) : 299-303.
  16. Benzie IFF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymology. 1999 ; 299 : 15-27. https://doi.org/10.1016/S0076-6879(99)99005-5
  17. Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 1986 ; 44(6) : 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  18. Park HJ, Jeong PK, Lim JT, Kwon PS. The current status of cultivation of Alisma plantago L. in Seung-Ju, Korea. Korean. J. Medicinal. Crop Sci. 1993 ; 1(2) : 202-204.
  19. Jang IW, Jeong JK, Kim HS, Lee SI. The literature study of research trend of Alismatis Rhizoma and relationship between the Herbology and KCD. Kor. J. Herbol. 2016 ; 31(2) : 47-62. https://doi.org/10.6116/kjh.2016.31.2.47.
  20. Choi YK, Sul JU, Park SK, Yu SN, Kim SH, Rhee MS, Ahn SC, Shin MS. Research trends of fermented medicinal herbs - Based on their clinical efficacy and safety assessment. J. Life Sci. 2012 ; 22(12) : 1729-1739. https://doi.org/10.5352/JLS.2012.22.12.1729
  21. Cho BJ, Hong JY, Kim MJ, Song YO. Development of mouthwash products with solid fermented oriental medicinal herb. Korean J. Food & Nutr. 2014 ; 43(9) : 1380-1387. https://doi.org/10.3746/jkfn.2014.43.9.1380
  22. Park EK, Sung JH, Trinh HT, Bae EA, Yun HK, Hong SS, Kim DH. Lactic acid bacterial fermentation increases the antiallergic effects of Ixeris dentate. J. Microbiol. Biotechnol. 2008 ; 18(2) : 308-313.
  23. Oh CS, Kim MS, Kim Il Kim HY, Park SI, Choi SI, Hong DS. Acne treatment cases with Hwangryeonhaedok-tang. J. Korean Med. Ophthalmol Otolaryngol Dermatol. 2009 ; 22(3) : 228-236.
  24. Son MY. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Indus Nutr. 2007 ; 12(2) : 51-57.
  25. Lee DS, Kang MS, Kim YC, Im NK, Kim HS, Jeong GS. Functionality analysis of Rhus javanica fermented by Lactobacillus spp. J. Life Sci. 2013 ; 23(1) : 44-54. https://doi.org/10.5352/JLS.2013.23.1.44
  26. Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 2004 ; 134(12) : 3479S-3485S. https://doi.org/10.1093/jn/134.12.3479S
  27. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol. 2005 ; 37(2) : 233-240.
  28. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean J. Food Sci. Technol. 2012 ; 44(3) : 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  29. Lee ER, Kang GH, Kang YJ, Kim WY, Choi HY, Kim BW. Regulation of cellular signal transduction by flavonoids. J. Cancer Prev. 2007; 12(3): 163-173.
  30. Hertog MG, Hollman PC, Van de Putte B. Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices. J. Agric. Food Chem. 1993 ; 41(8) : 1242-1246. https://doi.org/10.1021/jf00032a015
  31. Choi MH, Shin HJ. Anti-oxidative and antimelnogenesis effects of buleberry extract. Asian J. Beauty Cosmetol. 2015 ; 13(2) : 261-266.
  32. Choe M, Kim DJ, Lee HJ, You JK, Seo DJ, Lee JH. A study on the glucose-regulating enzymes and antioxidant activities of water extracts from medicinal herbs. Korean J. Food & Nutr. 2008 ; 37(5) : 542-547. https://doi.org/10.3746/jkfn.2008.37.5.542
  33. Lee SN, Kim MG, Kim MH, Kim HJ, Jo HJ, Kim EH. Effects of Alismatis Rhizoma pharmacopuncture extracts on the elastase activity and DPPH and NO scavenging activities. Korean J. Acupunct. 2011 ; 28(1) : 15-22.
  34. Choi GY, Kim BW. Experimental study on the antioxidant and antimicrobial properties of Dioscoreae Rhizoma. J. Int. Korean Med. 2010 ; 31(2) : 290-297.
  35. Choi JI, Kim YJ, Kim JH, Song BS, Yoon YH, Byun MW. Antioxidant activities of the extract fractions from Suaeda japonica. Korean J. Food & Nutr. 2009 ; 38(2) : 131-135. https://doi.org/10.3746/jkfn.2009.38.2.131
  36. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 1996 ; 239(1) : 70-76. https://doi.org/10.1006/abio.1996.0292
  37. Lee YJ, Yoon BR, Kim DB, Kim MD, Lee DW, Kim JK. Antioxidant activity of fermented wild grass extracts. Korean J. Food & Nutr. 2012 ; 25(2) : 407-412. https://doi.org/10.9799/ksfan.2012.25.2.407
  38. Jeong CH, Shim KH. Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. Korean J. Food & Nutr. 2006 ; 35(5) : 511-515. https://doi.org/10.3746/jkfn.2006.35.5.511
  39. Song WY, Chun SS, Choi JH. Antioxidant activities of selenium-treated Spinacia oleracea L. J. Food Hyg. Saf. 2018 ; 33(6) : 510-515. https://doi.org/10.13103/JFHS.2018.33.6.510
  40. Yim CY. Nitric oxide and cancer. Korean J. Med. 2010 ; 78(4) : 430-436.
  41. Kim DS, Sung NY, Park SY, Kim G, Eom J, Yoo JG. Immunomodulating activity of Sargassum horneri extracts in RAW264.7 macrophages. J. Nutr. Health. 2018 ; 51(6) : 507-514. https://doi.org/10.4163/jnh.2018.51.6.507
  42. Lee SJ, Rim HK, Jung JY, An HJ, Shin JS, Cho CW. Immunostimulatory activity of polysaccharides from Cheonggukjang. Food Chem. Toxicol. 2013 ; 59 : 476-484. https://doi.org/10.1016/j.fct.2013.06.045