Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Occurrence of a Natural Intergeneric Hybrid between a Female Tanakia lanceolata and a Male Rhodeus pseudosericeus (Cypriniformes: Cyprinidae) in Daecheoncheon Stream Flowing into the Yellow Sea in the Republic of Korea

서해안 독립 하천 대천천에서 납자루 Tanakia lanceolata (♀)와 한강납줄개 Rhodeus pseudosericeus(♂)의 자연 속간잡종 출현

  • Kim, Yong Hwi (Department of Life Science, Soonchunhyang University) ;
  • Sung, Mu Sung (Department of Life Science, Soonchunhyang University) ;
  • Yun, Bong Han (Department of Life Science, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Life Science, Soonchunhyang University)
  • 김용휘 (순천향대학교 생명과학과) ;
  • 성무성 (순천향대학교 생명과학과) ;
  • 윤봉한 (순천향대학교 생명과학과) ;
  • 방인철 (순천향대학교 생명과학과)
  • Received : 2021.02.24
  • Accepted : 2021.04.01
  • Published : 2021.06.30
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Abstract

A male, presumed to be an intergeneric hybrid between Tanakia lanceolata and Rhodeus pseudosericeus, was collected in the Boryeong Daecheoncheon Stream flowing into the Yellow Sea in the Republic of Korea. Morphological and molecular phylogenetic analyses were performed to discriminate the definite origin of the estimated natural hybrid. As a result of the morphological analysis, the color of the dorsal and anal fin rays edges of the natural hybrid individual, the upper and lower body colors followed the morphological characteristics of T. lanceolata, and that blue longitudinal stripe in the center of the caudal peduncle, the incomplete lateral line, and the barbels absent followed the morphological characteristics of R. pseudosericeus. In addition, as a result of the cytochrome b (cytb) gene analysis of mitochondrial DNA (mtDNA), the natural hybrid showed a nucleotide sequence similarity of 99.82 to 100% with T. lanceolata, and the maternal species was identified as T. lanceolata. As a result of the recombination activating gene 1 (rag1) gene analysis of nuclear DNA (nDNA), the natural hybrid showed double peaks pattern reflecting both the single nucleotide polymorphism sites (38 bp) between T. lanceolata and R. pseudosericeus, and the paternal species was identified as R. pseudosericeus. Therefore, a natural hybrid estimated male of Acheilognathinae analyzed in this study was found to be an intergeneric hybrid between a female T. lanceolata and a male R. pseudosericeus.

납자루 Tanakia lanceolata와 한강납줄개 Rhodeus pseudosericeus 간의 속간잡종으로 추정되는 수컷 1개체를 서해안 독립 하천인 보령 대천천에서 채집하였다. 해당 속간잡종 개체의 명확한 기원을 판별하기 위하여 형태학적 및 분자계통학적 분석을 수행하였다. 형태학적 분석 결과, 속간잡종 개체의 등지느러미 앞쪽 상단과 뒷지느러미 가장자리의 색상, 등과 배 쪽 색상 등은 납자루의 형태적 특징을 따랐으며, 미병부 중앙에 파란색 체측종대가 존재하는 점, 측선이 불완전한 점, 입수염이 없는 점 등은 한강납줄개의 형태적 특징을 따랐다. 또한, 미토콘드리아 DNA의 cytb 유전자 분석 결과, 속간잡종 개체는 납자루와 99.82~100%의 염기서열 유사도를 나타내어, 모계 종은 납자루로 판단되었다. 핵 DNA의 rag1 유전자 분석 결과, 속간잡종 개체는 납자루와 한강납줄개 간의 단일염기다형성 부위(38 bp)를 모두 반영하는 double peaks 양상을 나타내어, 부계 종은 한강납 줄개로 판단되었다. 따라서 본 연구에서 분석한 납자루아과 자연 잡종 추정 개체는 암컷 납자루와 수컷 한강납줄개 간의 속간잡종으로 판명되었다.

Keywords

Acknowledgement

이 논문은 순천향대학교의 연구비를 지원받아 수행된 연구입니다. 본 연구에서 실험 재료와 현지 채집에 도움을 주신 보령민물생태관의 조성장 관장님께 감사의 말씀을 전해드립니다.

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