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DNA Barcoding of Benthic Ragworms of the Genus Nectoneanthes (Polychaeta: Nereididae) Collected in Korean Waters

  • Received : 2021.07.03
  • Accepted : 2021.07.07
  • Published : 2021.07.31

Abstract

To provide better taxonomic information of the genus Nectoneanthes, the two DNA barcode regions of mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA (rDNA) sequences of Nectoneanthes oxypoda and N. uchiwa were determined. In addition, the respective sequences of four nereidid species closely related to Nectoneanthes were retrieved from GenBank for comparison and to estimate intra- and inter-specific genetic distances. The aligned sequence lengths of COI and 16S rDNA were 570 bp and 419 bp long, respectively. The mean intraspecific variation in both markers was less than 1% in all species except for that in COI of H. diadroma (1.87%). The mean interspecific variation between N. oxypoda and N. uchiwa was 12.02% regarding COI and 1.85% regarding 16S rDNA. In contrast, the mean interspecific variation between species of other genera was comparably higher(i.e., genus Perinereis: 20.5% in COI and 8.3% in 16S rDNA; genus Hediste: 13.18% in COI and 2.64% in 16S rDNA), compared with that between the two Nectoneanthes species. This result indicated that these Nectoneanthes species are genetically more closely related than other congeneric species of different genera. The DNA barcoding information on Nectoneanthes species generated in this study provides valuable insights for further biodiversity studies on nereidid species.

Keywords

Acknowledgement

The author would like to thank Ms. Sunghee Jung for producing raw sequence data for the present study. The author is also grateful to Ms. Yun Kyoung Kim for her assistance with the drawings. This work was supported by a grant (NIBR202010102) from the National Institute of Biological Resources(NIBR), Ministry of Environment of the Republic of Korea.

References

  1. Alves PR, Halanych KM, Santos CSG, 2020. The phylogeny of Nereididae (Annelida) based on mitochondrial genomes. Zoologica Scripta, 49:366-378. https://doi.org/10.1111/zsc.12413
  2. Bakken T, Wilson RS, 2005. Phylogeny of nereidids(Polychaeta, Nereididae) with paragnaths. Zoologica Scripta, 34:507-547. https://doi.org/10.1111/j.1463-6409.2005.00200.x
  3. Carr CM, Hardy SM, Brown TM, Macdonald TA, Hebert PDN, 2011. Tri-oceanic perspective: DNA barcoding reveals geographic structure and cryptic diversity in Canadian polychaetes. PLoS ONE, 6:e22232. https://doi.org/10.1371/journal.pone.0022232
  4. Choi JW, Seo JY, Lee CH, Ryu TK, Sung CG, Han GM, Hyun S, 2005. Spatial distribution patterns of macrobenthic communities during winter and summer in the Masan Bay special management area, southern coast of Korea. Ocean and Polar Research, 27:381-395 (in Korean, English summary). https://doi.org/10.4217/OPR.2005.27.4.381
  5. Dales RP, 1962. The polychaete stomodeum and the inter-relationships of the families of Polychaeta. Proceedings of the Zoological Society London, 139:389-428. https://doi.org/10.1111/j.1469-7998.1962.tb01837.x
  6. de Blainville HMD, 1818. Memoire sur la classe des Setipodes, partie des Vers a sang rouge de M. Cuvier, et des Annelides de M. de Lamarck. Bulletin des Sciences, par la Societe Philomatique de Paris, 1818:78-85.
  7. Hsueh PW, 2018. First records of Composetia, Eunereis and Nectoneanthes (Annelida: Nereididae) from Taiwan, with descriptions of two new species. Zootaxa, 4531:211-224. http://doi.org/10.11646/zootaxa.4531.2.3
  8. Imajima M, 1972. Review of the annelid worms of the family Nereidae of Japan, with descriptions of five new species or subspecies. Bulletin of the National Science Museum, Tokyo, 15:37-153.
  9. Izuka A, 1908. On the breeding habit and development of Nereis japonica n. sp. Annotationes Zoologicae Japonenses, 6:295-305.
  10. Izuka A, 1912. The errantiate polychaeta of Japan. Journal of the College of Science, Imperial University of Tokyo, 30:1-262.
  11. Kinberg JGH, 1865. Annulata nova. [Continuatio.]. Ofversigt af Koniglich Vetenskapsakademiens Forhandlingar, Stockholm, 22:167-179.
  12. Lee JH, Park JY, Lee HG, Park HS, Kim D, 2003. Environmental assessment of the Shihwa Lake by using the benthic pollution index. Ocean and Polar Research, 25:183-200 (in Korean, English summary). https://doi.org/10.4217/OPR.2003.25.2.183
  13. Leuckart R, 1847. Verzeichniss der zur Fauna Helgoland's gehorenden wirbellosen Seethiere. In: Beitrage zur Kenntniss wirbelloser Thiere (Eds., Frey H, Leuckart R). Friedrich Vieweg und Sohn, Braunschweig, pp. 136-168.
  14. Marenzeller E, 1879. Sudjapanische Anneliden I. Amphinomea, Aphroditea, Lycoridea, Phyllodocea, Hesionea, Syllidea, Eunicea, Glycerea, Sternaspidea, Chaetopterea, Cirratulea, Amphictenea. Denkschriften der Mathematisch-naturwissenschaftlichen Classe der Kaiserliche Akademie der Wissenschaften, 41:109-154 (in German).
  15. Paik EI, 1972. The polychaetous annelids in Korea (I). Bulletin of the Korean Fisheries Society, 5:128-136.
  16. Paik EI, 1973. The polychaetous annelids in Korea (II). Description of Nectoneanthes latipoda sp. nov. Bulletin of the Korean Fisheries Society, 6:81-84.
  17. Palumbi SR, 1996. Nucleic acids II: the polymerase chain reaction. In: Molecular systematics(Eds., Hillis DM, Moritz C, Mable BK). Sinauer Associates, Sunderland, MA, pp. 205-247.
  18. Park TS, Kim W, 2007. A taxonomic study on Perinereis nuntia species group (Polychaeta: Nereididae) of Korea. Korean Journal of Systematic Zoology, 23:75-85. https://doi.org/10.5635/KJSZ.2007.23.1.075
  19. Park T, Kim W, 2017. Description of a new species for Asian populations of the "Cosmopolitan" Perinereis cultrifera (Annelida: Nereididae). Zoological Science, 34:252-260. https://doi.org/10.2108/zs160154
  20. Sampieri BR, Vieira PE, Teixeira MAL, Seixas VC, Pagliosa PR, Amaral ACZ, Costa FO, 2021. Molecular diversity within the genus Laeonereis (Annelida, Nereididae) along the west Atlantic coast: paving the way for integrative taxonomy. PeerJ, 9:e11364. https://doi.org/10.7717/peerj.11364
  21. Sato M, 2013. Resurrection of the genus Nectoneanthes Imajima, 1972 (Nereididae: Polychaeta), with redescription of Nectoneanthes oxypoda (Marenzeller, 1879) and description of a new species, comparing them to Neanthes succinea (Leuckart, 1847). Journal of Natural History, 47:1-50. https://doi.org/10.1080/00222933.2012.743609
  22. Sato M, Nakashima A, 2003. A review of Asian Hediste species complex (Nereididae, Polychaeta) with descriptions of two new species and a redescription of Hediste japonica (Izuka, 1908). Zoological Journal of the Linnean Society, 137:403-445. https://doi.org/10.1046/j.1096-3642.2003.00059.x
  23. Sayers EW, Cavanaugh M, Clark K, Ostell J, Pruitt KD, Karsch-Mizrachi I, 2020. GenBank. Nucleic Acids Research, 48:D84-D86. https://doi.org/10.1093/nar/gkz956
  24. Stecher G, Tamura K, Kumar S, 2020. Molecular evolutionary genetics analysis (MEGA) for macOS. Molecular Biology and Evolution, 37:1237-1239. https://doi.org/10.1093/molbev/msz312
  25. Tosuji H, Nishinosono K, Hiseh HL, Glasby CJ, Sakaguchi T, Sato M, 2019. Molecular evidence of cryptic species diversity in the Perinereis nuntia species group (Annelida: Nereididae) with first records of P. nuntia and P. shikueii in southern Japan. Plankton and Benthos Research, 14:287-302. https://doi.org/10.3800/pbr.14.287
  26. Treadwell AL, 1936. Polychaetous annelids from Amoy, China. Proceedings of the United States National Museum, 83:261-279. https://doi.org/10.5479/si.00963801.83-2984.261
  27. Villalobos-Guerrero TF, Park T, Idris I, 2021. Review of some Perinereis Kinberg, 1865 (Annelida: Nereididae) species of Group 2 sensu Hutchings, Reid & Wilson, 1991 from the Eastern and South-eastern Asian seas. Journal of the Marine Biological Association of the United Kingdom, 101:279-307. https://doi.org/10.1017/S0025315421000126
  28. Wilson RS. 1988. Synonymy of the genus Nectoneanthes Imajima, 1972, with Neanthes Kinberg, 1866 (Polychaeta: Nereididae). Proceedings of the Biological Society of Washington, 101:4-10.