Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Comparison of morphological characteristics of the river puffer, Takifugu obscurus, the tiger puffer, T. rubripes, their hybrids, and their triploid hybrids

  • Park, In-Seok (Division of Convergence on Marine Science, College of Ocean Science and Engineering, Korea Maritime & Ocean University) ;
  • Oh, Ji Su (National Agency for Marine Seed Improvement, National Institute of Fisheries Science)
  • Received : 2021.01.04
  • Accepted : 2021.02.08
  • Published : 2021.03.31
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Abstract

Various biometric and geometric measures were used to discriminate between the morphologically similar river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids, and their triploid hybrids. The hybrids and triploid hybrids had greater anal fin width, nostril width, and snout length than the parental species (p<0.05). However, they had less caudal peduncle depth, inter-orbital width, head length, and head width(p<0.05). The morphometric and meristic characteristics of the hybrids and triploid hybrids were either intermediate between the parental species or more similar to those of one parental species. However, the external morphology of the hybrids and triploid hybrids was predominantly maternal. The triploid hybrids had asymmetry in the fin rays and gill raker numbers. This study identified phenotypic characteristics by distinguishing the morphological variables of river puffer, tiger puffer, their hybrids, and their triploid hybrids.

Keywords

Acknowledgement

This manuscript has markedly benefited from the comments of anonymous reviewers. The method used to produce hybrids between the river puffer, Takifugu obscurus, and the tiger puffer, T. rubripes, is the subject of a Republic of Korean patent (Title: Production method of hybrid between river puffer, T. obscurus, and tiger puffer, T. rubripes; Patent No. 10-1632842; June 2016). We are grateful to the staff of the Fishery Genetics and Breeding Sciences Laboratory of the Korea Maritime & Ocean University, Republic of Korea.

References

  1. Albertson RC and TD Kocher. 2001. Assessing morphological differences in adaptive trait: a landmark-based morphometric approach. J. Exp. Zool. 289:385-403. https://doi.org/10.1002/jez.1020
  2. Benfey TJ. 1999. The physiology and behavior of triploid fishes. Fish. Sci. 7:39-67.
  3. Bonar SA, GL Thomas and GB Pauley. 1988. Evaluation of the separation of triploid and diploid grass carp (Ctenopharyngodon idella) (Valenciennes), by external morphology. J. Fish. Biol. 33:895-898. https://doi.org/10.1111/j.1095-8649.1988.tb05537.x
  4. Bonnet S, P Haffray, JM Blanc, F Vallee, C Vauchez, A Faure and B Fauconneau. 1999. Genetic variation in growth parameters until commercial size in diploid and triploid freshwater rainbow trout(Oncorhynchus mykiss) and seawater brown trout (Salmo trutta). Aquaculture 173:359-375. https://doi.org/10.1016/S0044-8486(98)00460-8
  5. Caillon F, V Bonhomme, C Mollmann and R Frelat. 2018. A morphometric dive into fish diversity. Ecosphere 9:e02220. https://doi.org/10.1002/ecs2.2220
  6. Cardin SX. 2000. Advances in morphometric identification of fishery stocks. Rev. Fish Biol. Fish. 10:91-112. https://doi.org/10.1023/A:1008939104413
  7. Chevassus B. 1983. Hybridization in fish. Aquaculture 33:245-262. https://doi.org/10.1016/0044-8486(83)90405-2
  8. Dou D, X Wang, H Zhu, Y Bao, Y Wang, J Cui, X Meng, Y Zhu and X Qiu. 2019. The complete mitochondrial genome of the hybrid of Takifugu obscurus (♀)×Takifugu rubripes (♂). Mitochondrial DNA Part B-Resour. 4:3196-3197. https://doi.org/10.1080/23802359.2019.1669082
  9. Duncan DB. 1955. Multiple range and multiple F test. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  10. Dunham RA, RO Smitherman, MJ Brooks, M Benchakan and JA Chappell. 1982. Paternal predominance in reciprocal channelblue hybrid catfish. Aquaculture 29:389-396. https://doi.org/10.1016/0044-8486(82)90152-1
  11. Gray AK, MA Evans and GH Thorgaard. 1993. Viability and development of diploid and triploid salmonid hybrids. Aquaculture 112:125-142. https://doi.org/10.1016/0044-8486(93)90439-6
  12. Hamasaki M, Y Takeuchi, K Miyaki and G Yoshizaki. 2013. Gonadal development and fertility of triploid grass puffer, Takifugu niphobles induced by cold shock treatment. Mar. Biotechnol. 15:133-144. https://doi.org/10.1007/s10126-012-9470-3
  13. Kang HW, KB Shim, DY Kang, KC Jo, KC Song, JH Lee, HI Song, SG Son and YJ Cho. 2007. Sitological quality evaluation of cultured and wild river puffer, Takifugu obscurus. J. Aquacult. 20:147-153.
  14. Kasama M and H Kobayasi. 1991. Hybridization experiment between Gnathopogon elongatus elongatus (♀) and Carassius carassius (♂). Jpn. J. Ichthyol. 38:295-300. https://doi.org/10.1007/BF02905575
  15. Kim DS, YK Nam and IS Park. 1995. Survival and karyological analysis of reciprocal diploid and triploid hybrids between mud loach (Misgurnus mizolepis) and cyprinid loach (M. anguillicaudatus). Aquaculture 135:257-265. https://doi.org/10.1016/0044-8486(95)01031-9
  16. Kim JH, KT Son, JS Mok, EG Oh, JK Kim and TK Lee. 2006. Toxicity of the puffer fish Takifugu porphyreus and T. rubripes from coastal areas of Korea. Korean J. Fish. Aquat. Sci. 39:447-453. https://doi.org/10.5657/KFAS.2006.39.6.447
  17. Kotaro K and F Takeshi. 2007. Growth of tiger puffer, Takifugu rubripes at different salinities. J. World. Aquacult. Soc. 38:427-434. https://doi.org/10.1111/j.1749-7345.2007.00114.x
  18. Leary RF, FW Allendorf, KL Knudsen and GH Thorgaard. 1985. Heterozygosity and developmental stability in gynogenetic diploid and triploid rainbow trout. Heredity 54:219-225. https://doi.org/10.1038/hdy.1985.29
  19. Lim SG, MH Jeong, BS Kim, TH Lee, HW Gil and IS Park. 2016. Landmark-based morphometric and meristic analysis of Serranidae. Dev. Reprod. 20:73-85. https://doi.org/10.12717/DR.2016.20.2.073
  20. Mojekwu TO and CI Anumudu. 2015. Advanced techniques for morphometric analysis in fish. J. Aquacult. Res. Dev. 6:354.
  21. Normala J, AA Mohd, MAB Abol, AA Nur, W Khor, TV Okomoda and MS Shahreza. 2017. Morphometric variations between triploid and diploid Clarias gariepinus(Burchell, 1822). Croat. J. Fish. 75:113-121. https://doi.org/10.1515/cjf-2017-0015
  22. Nunez-Vazquez EJ, A Garcia-Ortega, AI Campa-Cordova, IA Parra, L Ibarra-Martinez, A Heredia-Tapia and JL Ochoa. 2012. Toxicity of cultured bullseye puffer fish, Sphoeroides annulatus. Mar. Drugs 10:329-339. https://doi.org/10.3390/md10020329
  23. Park IS. 2019. A comparative analysis of cell cycles in diploid and induced triploid tissues in marine medaka (Oryzias dancena). Korean J. Environ. Biol. 37:735-740. https://doi.org/10.11626/KJEB.2019.37.4.735
  24. Park IS. 2020a. Morphometric characteristics of diploid and triploid Far Eastern catfish, Silurus asotus. Korean J. Environ. Biol. 38:106-113. https://doi.org/10.11626/KJEB.2020.38.1.106
  25. Park IS. 2020b. Comparative analysis of sectioned-body morphometric characteristics of diploid and triploid marine medaka Oryzias dancena. Korean J. Environ. Biol. 38:137-145. https://doi.org/10.11626/KJEB.2020.38.1.137
  26. Park IS and CI Zhang. 1994. Morphometrical differences between diploid and induced triploid cherry salmon, Oncorhynchus masou. Korean J. Ichthyol. 6:206-221.
  27. Park IS and HW Gil. 2018. Comparative analysis of fluctuating asymmetry between ploidy and sex in marine medaka, Oryzias dancena. Dev. Reprod. 22:275-281. https://doi.org/10.12717/DR.2018.22.3.275
  28. Park IS, GC Choi and DS Kim. 1997. Production of hybrid and allotriploid between rainbow trout, Oncorhynchus mykiss and cherry salmon, O. masou. II. Characteristics of sex ratio and morphometric traits. J. Aquacult. 10:49-54.
  29. Park IS, HW Gil and JS Oh. 2019. Changing salinity affects hematological and histological response in hybrids and hybrid triploids between river puffer, Takifugu obscurus and tiger puffer, T. rubripes. Dev. Reprod. 23:239-253. https://doi.org/10.12717/DR.2019.23.3.239
  30. Park IS, HW Gil, JS Oh, HJ Choi and CH Kim. 2015. Comparative analysis of morphometric characteristics of Scorpaenidae and Gobioninae. Dev. Reprod. 19:85-96. https://doi.org/10.12717/DR.2015.19.2.085
  31. Park IS, SY Lim, TH Lee, HW Gill and GY Yoo. 2017. Various characteristics of hybrid between river puffer, Takifugu obscurus and tiger puffer, T. rubripes, and their hybrid triploid. Dev. Reprod. 21:181-191. https://doi.org/10.12717/DR.2017.21.2.181
  32. Park IS, YK Nam and DS Kim. 2006. Growth performance, morphometric trait and gonad development of induced reciprocal diploid and triploid hybrids between the mud loach(Misgurnus mizolepis) and cyprinid loach (Misgurnus anguillicaudatus). Aquacult. Res. 37:1246-1253. https://doi.org/10.1111/j.1365-2109.2006.01556.x
  33. Peruzzi S, B Chatain, E Saillant, P Haffray, B Menu and JC Falguiere. 2004. Production of meiotic gynogenetic and triploid sea bass, Dicentrarchus labrax L.: 1. Performances, maturation and carcass quality. Aquaculture 230:41-64. https://doi.org/10.1016/S0044-8486(03)00417-4
  34. Rawat S, S Benakappa, J Kumar, ASK Naik, G Pandey and CW Pema. 2017. Identification of fish stocks based on truss morphometric: A review. J. Fish. Life Sci. 2:9-14.
  35. Scheerer PD and GH Thorgaard. 1983. Increased survival in salmonid hybrids by induced triploidy. Can. J. Fish. Aquat. Sci. 40:2040-2044. https://doi.org/10.1139/f83-235
  36. Solar II, EM Donaldson and GA Hunter. 1984. Induction of triploidy in rainbow trout (Salmo gairdneri Richardson) by heat shock, and investigation of early growth. Aquaculture 42:57-67. https://doi.org/10.1016/0044-8486(84)90313-2
  37. Strauss RE and CE Bond. 1990. Taxonomic methods: Morphology. pp. 125-130. In: Methods for Fish Biology (Schreck CB and PB Moyle eds.). American Fisheries Society. Bethesda, MD.
  38. Strauss RE and FL Bookstein. 1982. The truss: body from reconstructions in morphometrics. Syst. Zool. 31:113-135. https://doi.org/10.2307/2413032
  39. Strussmann CA and F Takashima. 1993. Hepatocyte nuclear size and nutritional condition of larval pejerrey (Odontesthes bonariensis)(Cuvier et Valenciennes). J. Fish. Biol. 36:59-65. https://doi.org/10.1111/j.1095-8649.1990.tb03519.x
  40. Thorgaard GH, PS Rabinovitch, MW Shen, GAE Gall, J Propp and FM Utter. 1982. Triploid rainbow trout identified by flow cytometry. Aquaculture 29:305-309. https://doi.org/10.1016/0044-8486(82)90144-2
  41. Turan C. 1999. A note on the examination of morphometric differentiation among fish populations: The truss system. Turk. J. Zool. 23:259-263.
  42. Wilkins NP, E Gosling, A Curatolo, A Linnane, C Jordan and HP Courtney. 1995. Fluctuating asymmetry in Atlantic salmon, European trout and their hybrids, including triploids. Aquaculture 137:77-85. https://doi.org/10.1016/0044-8486(95)01099-8
  43. Yang Z and Y Chen. 2003. Length-weight relationship of obscure puffer (Takifugu obscurus) during spawning migration in the Yangtze River, China. J. Freshw. Ecol. 18:349-352. https://doi.org/10.1080/02705060.2003.9663969
  44. Yoo GY, TH Lee, HW Gill, SG Lim and IS Park. 2018. Cytogenetic analysis of hybrids and hybrid triploids between the river puffer, Takifugu obscurus and the tiger puffer, T. rubripes. Aquacult. Res. 49:637-650. https://doi.org/10.1111/are.13493