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Morphological Development of Egg and Larvae of Squalidus japonicus coreanus (Gobioninae)

몰개(Squalidus japonicus coreanus)의 난 발생 및 자치어의 형태 발달

  • Kim, Kyeong-Mu (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kwak, Yeong-Ho (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Seung-Yong (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Song, Ha-Yun (Inland Fisheries Research Institute, National Institute of Fisheries Science)
  • 김경무 (국립수산과학원 중앙내수면연구소) ;
  • 곽영호 (국립수산과학원 중앙내수면연구소) ;
  • 김승용 (국립수산과학원 중앙내수면연구소) ;
  • 송하윤 (국립수산과학원 중앙내수면연구소)
  • Received : 2021.08.10
  • Accepted : 2021.09.25
  • Published : 2021.12.31

Abstract

The egg development and early life history of short barbel gudgeon Squalidus japonicus coreanus were investigated. For the experiments, the mature adults were collected at the stream Jicheon in Korea. The eggs from the females were obtain by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 1.12±0.03 mm (1.10~1.16 mm, n=30) in diameter. The embryo began to hatch about 49 hrs after fertilization under water temperature of 23±1℃. The newly-hatched larvae (Yolksac larva) were 3.7±0.1 mm (3.4~3.8 mm, n=16) in total length, and they haven't Melanophore. 5 days after hatching, the Preflexion larva were 5.3±0.2 mm (5.0~5.5 mm, n=16) in total length, and they began to eat a Rotifer. 19 days after hathing, the Flexion larva were 6.0±0.3 mm (5.4~6.5 mm, n=16) in total length, and they began to eat a Brine shrimp. 29 days after hatching, the Postflexion larva were 9.6±0.5 mm (8.3~10.5 mm, n=16) in total length, and dorsal fin rays are were formed. 44 days after hatching, the juvenile were 15.5±1.0 mm (13.5~17.0 mm, n=16) in total length, and all their fin-rays were formed.

본 연구는 난과 자치어의 형태 및 생활사가 밝혀지지 않은 Squalidus japonicus coreanus의 초기생활사에 대하여 연구하였다. S. japonicus coreanus의 친어는 충청남도 공주시의 지천에서 확보하였다. 성숙한 암컷 친어에 HCG (human chorionic gonadotropin)를 10 IU/g의 농도로 주사하여 성숙란을 얻었으며, 습식법으로 인공 수정하였다. 수정란은 불투명한 흰색 원형의 침성점착란으로 난경은 1.12±0.03 mm (1.10~1.16 mm, n=30)였다. 수정란은 수온 23±1℃에서 수정 49시간 후 부화가 완료되었으며, 난황자어의 전장은 3.7±0.1 mm (3.4~3.8 mm, n=16)로 흑색소포가 관찰되지 않았다. 부화 5일 후 자어의 전장은 5.3±0.2 mm (5.0~5.5 mm, n=16)로 난황의 흡수가 완료되고 윤충류 섭식을 시작하여 전기자어기로 이행하였다. 부화 19일째의 자어의 전장 6.0±0.3 mm (5.4~6.5 mm, n=16)로 척삭말단이 굴곡이 시작되고 꼬리지느러미의 기조가 형성되어 중기자어기로 이행하였다. 부화 29일 후 자어의 전장은 9.6±0.5 mm (8.3~10.5 mm, n=16)로 척색말단의 굴곡이 완료되어 후기자어기로 이행하였다. 부화 44일 후 전장 15.5±1.0 mm (13.5~17.0 mm, n=16)로 모든 지느러미의 기조가 정수에 도달하여 치어기로 이행하였다.

Keywords

Acknowledgement

본 연구는 2021년도 국립수산과학원 수산시험연구사업 내수면 수산자원 보호구역 환경 및 수산자원 연구(R2021036)의 지원으로 수행되었습니다.

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