Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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Comparision of antioxidant and anti-inflammatory activities of enzyme assisted hydrolysate from Ecklonia maxima blades and stipe

  • Lee, Hyo-Geun (Department of Marine Life Science, Jeju National University) ;
  • Je, Jun-Geon (Department of Marine Life Science, Jeju National University) ;
  • Hwang, Jin (Department of Marine Life Science, Jeju National University) ;
  • Jayawardena, Thilina U. (Department of Marine Life Science, Jeju National University) ;
  • Nagahawatta, D.P. (Department of Marine Life Science, Jeju National University) ;
  • Lu, Yu An (Department of Marine Life Science, Jeju National University) ;
  • Kim, Hyun-Soo (Marine Biodiversity Institute of Korea) ;
  • Kang, Min-Cheol (Research Group of Food Processing, Korea Food Research Institute) ;
  • Lee, Dae-Sung (Marine Biodiversity Institute of Korea) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
  • 투고 : 2021.03.20
  • 심사 : 2021.04.26
  • 발행 : 2021.05.31
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초록

Marine brown seaweeds are a source of functional ingredients with various biological properties. They have been used in the food and functional food industries. Brown seaweeds are divided into three parts of blades, stipe, and root. Normally seaweed blades were used as raw materials for biological research. However, there are limited uses on stipes of Ecklonia maxima (E. maxima) depending on the physicochemical, nutritional, and biological properties. Besides, the comparative studies of two structures of E. maxima, blades and stipe didn't discover previously. This study aimed to compare the potent antioxidant and anti-inflammatory activities of the two structures of E. maxima, blades and stipe in vitro studies to increase the utilization of the two structures of E. maxima. The enzyme-assisted hydrolysate from E. maxima showed significant antioxidant and anti-inflammatory activities. Among them, celluclast-assisted hydrolysate from E. maxima blades (EMBC) and viscozyme-assisted hydrolysate from E. maxima stipe (EMSV) expressed significant protection on hydrogen peroxide-induced oxidative stress. Moreover, EMBC and EMSV treatment remarkably reduced nitric oxide production by downregulation of pro-inflammatory cytokine expressions in lipopolysaccharide-stimulated Raw 264.7 cells. Especially EMBC showed strong inhibition on pro-inflammatory cytokine production compared to EMSV. Taken together research findings suggest that EMBC and EMSV possessed potent antioxidant and anti-inflammatory properties and may be utilized as functional ingredients in the food and functional food sectors.

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과제정보

This research was financially supported by a grant from the Marine Biotechnology program (20170488) funded by the Ministry of Oceans and Fisheries, Korea.

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