한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제38권3호
  • /
  • Pages.883-890
  • /
  • 2021
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

산딸기 잎 추출물이 멜라닌 생성에 미치는 영향

Effect of Rubus crataefifolius Leaf Extract on Melanin Synthesis

  • 김미경 (서원대학교 바이오코스메틱학과) ;
  • 김대용 (우석대학교 한의과대학)
  • Kim, Mee-Kyung (Department of Bio-Cosmetic Science, Seowon University) ;
  • Kim, Dae-Yong (College of Korean Medicine, Woosuk University)
  • 투고 : 2021.06.06
  • 심사 : 2021.06.30
  • 발행 : 2021.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 B16F10 melanoma 세포에서 산딸기 잎 추출물(Rubus crataefifolius Leaf Extract, RCLE)의 멜라닌 생성 억제 효과를 확인하고자 수행되었다. α-MSH로 자극한 B16F10 melanoma 세포에서 멜라닌 함량과 tyrosinase 활성, 멜라닌 생성관련 효소들인 TRP-1, TRP-2 및 MITF의 단백질 발현 수준을 조사하였고 이에 대한 RCLE의 효과를 검증하였다. RCLE는 tyrosinase 활성과 멜라닌 생성을 효과적으로 억제하였고 멜라닌 생성 경로에 관여하는 PKA와 CREB의 인산화와 MITF의 발현을 억제하였으며 멜라닌 생성 관련 단백질의 발현을 하향 조절하였다. 이러한 결과는 RCLE가 MITF 발현을 억제하여 α-MSH로 자극된 멜라닌 합성을 억제한다는 것을 보여준다. 따라서 이러한 연구결과는 RCLE가 과도한 멜라닌 생성으로 인한 색소 침착을 완화시킬 수 있는 기능성 화장품 소재로 활용될 수 있음을 시사한다.

In this study, we investigated the melanogenesis inhibitory effects of Rubus crataefifolius Leaf Extract (RCLE) in B16F10 melanoma cells. We examined the effects of RCLE on the melanin contents and tyrosinase activity, as well as the protein expression levels of the melanogenic enzymes TRP-1, TRP-2, and MITF in α-MSH -stimulated B16F10 melanoma cells. RCLE effectively inhibited tyrosinase activity and melanogenesis, suppressed the phosphorylation of PKA and CREB, and expression of MIT involved in the melanogenesis pathway, and down-regulated expression of melanogenesis related proteins. These result suggest that RCLE inhibited α-MSH-stimulated melanin synthesis by suppressing MITF expression. Therefore, our study suggests that RCLE has potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.

키워드

참고문헌

  1. S. C. Ko, S. H. Lee, "Protocatechuic Aldehyde Inhibits alpha-MSH-Induced Melanogenesis in B16F10 Melanoma Cells via PKA/CREB-Associated MITF Downregulation", International Journal of Molecular Sciences, Vol.22, No.8, pp. 3861, (2021). https://doi.org/10.3390/ijms22083861
  2. J. H. Ko, N. Lee, H. K. Kang, B. S. Kim, J. N. Lee, C. G. Hyun, "Anti-melanogenic effects of hot-water extracts from Torreya nucifera via MAPKs and cAMP signaling pathway on B16F10 cells", Die Pharmazie, Vol. 75, No.11, pp. 565-570, (2020).
  3. J. H. Lee, B. Lee, Y. D. Jeon, H. W. Song, Y. M. Lee, B. J. Song, D. K. Kim, "Inhibitory Effect of Elaeagnus umbellata Fractions on Melanogenesis in alpha-MSHStimulated B16-F10 Melanoma Cells", Molecules, Vol.26, No.5, pp. 1308, (2021). https://doi.org/10.3390/molecules26051308
  4. W. Ni, X. Zhang, H. Bi, J. Iteku, L. Ji, C. Sun, J. Fang, G. Tai, Y. Zhou, J. Zhao, "Preparation of a glucan from the roots of Rubus crataegifolius Bge. and its immunological activity", Carbohydrate Research, Vol.344, No.18 pp. 2512-2518, (2009). https://doi.org/10.1016/j.carres.2009.08.042
  5. Jung MS, Lee SJ, Song Y, Jang SH, Min W, Won CK, Kim HD, Kim TH, Cho JH. Rubus crataegifolius Bunge regulates adipogenesis through Akt and inhibits high-fat diet-induced obesity in rats. Nutrition & Metabolism, 13:29. 2016. https://doi.org/10.1186/s12986-016-0091-0
  6. J. U. Park, J. S. Cho, J. S. Kim, H. K. Kim, Y. H. Jo, M. A. A. Rahman, Y. I. Lee, "Synergistic Effect of Rubus crataegifolius and Ulmus macrocarpa Against Helicobacter pylori Clinical Isolates and Gastritis", Frontiers in Pharmacology, Vol.11, No.4, (2020).
  7. P. W. Wang, Y. C. Cheng, Y. C. Hung, C. H. Lee, J. Y. Fang, W. T. Li, Y. R. Wu, T. L. Pan, "Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties", Oxidative Medicine and Cellular Longevity, Vol.2019 pp. 9529676. (2019). https://doi.org/10.1155/2019/9529676
  8. P. Rattanawiwatpong, R. Wanitphakdeedecha, A. Bumrungpert, M. Maiprasert, "Anti-aging and brightening effects of a topical treatment containing vitamin C, vitamin E, and raspberry leaf cell culture extract: A split-face, randomized controlled trial", Journal of Cosmetic Dermatology, Vol.19, No.3, pp. 671-676, (2020). https://doi.org/10.1111/jocd.13305
  9. Y. C. Hseu, Y. Vudhya Gowrisankar, L. W. Wang, Y. Z. Zhang, X. Z. Chen, P. J. Huang, H. R. Yen, H. L. Yang, "The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways", Redox Biology, Vol.44 (2021).
  10. H. Y. Kim, G. Agrahari, M. J. Lee, L. J. Tak, W. K. Ham, T. Y. Kim, "Low-Temperature Argon Plasma Regulates Skin Moisturizing and Melanogenesis-Regulating Markers through Yes-Associated Protein". International Journal of Molecular Sciences, Vol.22, No.4, pp. 189, (2021).
  11. J. H. Ko, N. Lee, H. K. Kang, B. S. Kim, J. N. Lee, C. G. Hyun, "Anti-melanogenic effects of hot-water extracts from Torreya nucifera via MAPKs and cAMP signaling pathway on B16F10 cells", Die Pharmazie, Vol.75, No.11, pp. 565-570, (2020).
  12. Y. H. Kuo, C. C. Chen, P. Y. Wu, C. S. Wu, P. J. Sung, C. Y. Lin, H. M. Chiang, "N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms", BMC Complementary and Alternative Medicine, Vol.17, No.1, pp. 71, (2017). https://doi.org/10.1186/s12906-016-1554-6
  13. T. F. Tsang, B. Chan, W. C. Tai, G. Huang, J. Wang, X. Li, Z. H. Jiang, W. L. W. Hsiao, "Gynostemma pentaphyllum saponins induce melanogenesis and activate cAMP/PKA and Wnt/β-catenin signaling pathways", Phytomedicine, Vol.60 pp. 153008, (2019). https://doi.org/10.1016/j.phymed.2019.153008
  14. S. Y. Won, J. J. Park, E. Y. Shin, E. G. Kim, "PAK4 signaling in health and disease: defining the PAK4-CREB axis", Experimental & Molecular Medicine, Vol.51, No.2, pp. 1-9, (2019).
  15. W. Qian, W. Liu, D. Zhu, Y. Cao, A. Tang, G. Gong, H. Su, "Natural skin-whitening compounds for the treatment of melanogenesis (Review)", Experimental and Therapeutic Medicine, Vol.20, No.1, pp. 173-185, (2020). https://doi.org/10.3892/etm.2020.8687
  16. D. Fang, T. Kute, V. Setaluri, "Regulation of tyrosinase-related protein-2 (TYRP2) in human melanocytes: relationship to growth and morphology", Pigment Cell Research, Vol.14, No.2, pp.132-139, (2001). https://doi.org/10.1034/j.1600-0749.2001.140209.x
  17. A. Tuerxuntayi, Y. Q. Liu, A. Tulake, M. Kabas, A. Eblimit, H. A. Aisa, "Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells", BMC Complementary and Alternative Medicine, Vol.14, No.166, (2014).
  18. Y. C. Chung, C. G. Hyun, "Inhibitory Effects of Pinostilbene Hydrate on Melanogenesis in B16F10 Melanoma Cells via ERK and p38 Signaling Pathways", International Journal of Molecular Sciences, Vol.21, No.13, pp.4732, (2020). https://doi.org/10.3390/ijms21134732
  19. H. H. Ko, Y. T. Chang, Y. H. Kuo, C. H. Lin, Y. F. Chen, "Oenothera laciniata Hill Extracts Exhibits Antioxidant Effects and Attenuates Melanogenesis in B16-F10 Cells via Downregulating CREB/MITF/Tyrosinase and Upregulating p-ERK and p-JNK", Plants (Basel), Vol.10, No.4, pp. 727, (2021).