대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제47권2호
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  • Pages.179-184
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  • 2021
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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Serine 의 인공피부조직 투과 개선을 위한 Stearic Acid 기반 고형지질나노입자의 설계 연구

A Study on the Design of Stearic Acid-Based Solid Lipid Nanoparticles for the Improvement of Artificial Skin Tissue Transmittance of Serine

  • Yeo, Sooho (College of Pharmacy, Chung-Ang University)
  • 투고 : 2021.05.11
  • 심사 : 2021.06.15
  • 발행 : 2021.06.30
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초록

각질층은 피부의 가장 바깥쪽에 위치하여 피부 내 수분을 유지시킨다. 피부 보습은 각질층 내 천연보습인자(natural moisturizing factors, NMF)에 의존하는데, NMF 중 아미노산이 가장 많은 비율을 차지한다. 본 연구에서는 NMF 중 serine (Ser)의 피부 투과율을 개선시키기 위해 생체 적합한 고형지질인 stearic acid 기반 고형지질나노입자(solid lipid nanoparticles, SLNs)를 설계하였다. Ser 봉입 SLNs은 이중 가온용융유화법으로 제조하였다. 평균 입자 크기는 256.30 ~ 416.93 nm이었고 제타전위는 -17.60 ~ -35.27 mV이었다. 유화제의 지용성 또는 친수성의 정도가 각각 높아질수록 입자크기 작아지고 안정성 및 봉입율이 높아지는 경향을 보였다. Ser의 피부 투과 연구를 위해 인체 표피 유래 피부 조직(SkinEthicTM RHE)을 사용하였다. Ser의 피부 투과결과 SLN을 적용한 제형이 대조군인 Ser 용액에 비해 약 4.1 ~ 6.2 배 투과율이 개선되었음을 확인하였다. 유화제의 지용성 또는 친수성의 정도가 각각 높아질수록 Ser의 피부 투과율이 높아지는 경향을 보였다. 따라서, Ser이 봉입된 SLN은 기능성화장품의 보습효과 처방을 위한 경피흡수 제형으로 사용될 수 있을 것으로 기대된다.

Stratum corneum known as a skin barrier, which maintains water in skin, is the outer layer of the skin. Natural moisturizing factors (NMF) are one of the constituents in stratum corneum and amino acids are the highest components among NMF. In this study, we designed stearic acid-based solid lipid nanoparticles (SLNs) for improved skin penetration of serine (Ser). Ser-capsulated SLN was manufactured by double-melting emulsification method. The mean particle size and zeta potential of SLNs were 256.30 ~ 416.93 nm and -17.60 ~ -35.27 mV, respectively. The higher the degree of hydrophobicity or hydrophilicity of emulsifiers, the smaller the particle size and the higher the stability and capsulation rate. In addition, skin penetration was conducted using SkinEthicTM RHE which is one of the reconstructed human epidermis models. The results of Ser penetration demonstrated that all SLNs enhanced than serine solution. The amount of enhanced Ser penetration from SLNs were approximately 4.1 ~ 6.2 times higher than that from Ser solution. Therefore, Ser-loaded SLN might be a promising drug delivery system for moisturizing formulation in cosmeceutical.

키워드

참고문헌

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