한국어병학회지 (Journal of fish pathology)

  • 제34권1호
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  • Pages.105-110
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  • 2021
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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볼락(Sebastes inermis)에서 분리된 비브리오속 세균의 동정

Identification of Vibrio species isolated from dark-banded rockfish (Sebastes inermis)

  • 남우화 (강릉원주대학교 해양자원육성학과) ;
  • 박재린 (강릉원주대학교 해양자원육성학과) ;
  • 표지혜 (강릉원주대학교 해양자원육성학과) ;
  • 김정호 (강릉원주대학교 해양자원육성학과)
  • Nam, U-Hwa (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Park, Jae-Rin (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Pyo, Ji-Hye (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
  • 투고 : 2021.05.15
  • 심사 : 2021.05.28
  • 발행 : 2021.06.30
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초록

We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.

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참고문헌

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