Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Salicylamide Enhances Melanin Synthesis in B16F1 Melanoma Cells

  • Ito, Yusuke (Division of Animal Science, College of Agriculture, Tamagawa University) ;
  • Sato, Kazuomi (Division of Animal Science, College of Agriculture, Tamagawa University)
  • Received : 2020.12.09
  • Accepted : 2021.02.10
  • Published : 2021.07.01
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Abstract

Salicylamide, a non-steroidal anti-inflammatory drug (NSAID), is used as an analgesic and antipyretic agent. We have previously shown that several NSAIDs have anti-melanogenic properties in B16F1 melanoma cells. In contrast, we have found that salicylamide enhances melanin contents in B16F1 melanoma cells; however, the underlying mechanism is not known. Therefore, we investigated the mechanism through which salicylamide stimulates melanogenesis. Interestingly, salicylamide enhanced diphenolase activity in a cell-free assay. Western blotting and real-time RT-PCR revealed that salicylamide increased tyrosinase expression via transcriptional activation of the Mitf gene. Together, our results indicate that salicylamide could be used as an anti-hypopigmentation agent for skin and/or hair.

Keywords

Acknowledgement

We are grateful to Professor Keiko Ohashi-Kaneko of Tamagawa University and Mr. Takashi Nishio for their contributions to our work. We also thank Mr. Yuto Seki and Mr. Ryosuke Izumi for their technical assistance.

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