Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Effect of Plant Growth Regulators in In Vitro Culture of Hippophae rhamnoides

  • Lee, Songhee (Department of Biological and Environmental Science, Dongguk University) ;
  • Cho, Wonwoo (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Jang, Hyeonsoo (Crop Production & Physiology Div., National Institute of Crop Science) ;
  • Chandra, Romika (Department of Biological and Environmental Science, Dongguk University) ;
  • Lee, Sora (Forest Biomaterials Research Center, National Institute of Forest Science) ;
  • Kang, Hoduck (Department of Biological and Environmental Science, Dongguk University)
  • Received : 2020.11.25
  • Accepted : 2021.02.18
  • Published : 2021.06.30
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Abstract

This study was carried out to establish in vitro propagation system influenced by plant growth regulators through organogenesis with three different seed sources (China, Mongolia and Russia) for conservation of genetic resources in Northeast Asia. The experiment compared two different carbon sources (commercial sugar, sucrose), which showed no significant differences in germination rate. Induced adventitious buds from leaf segments were found to be highly effective when supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin, and 5.0 mg/L IAA, in the case of Chinese origin 96.8%, Russian origin R-1: 95.6%, R-2: 85.6%, and Mongolian origin M-2: 77.8%. It was effective in BA and Kinetin with supplemented with IAA, respectively. Shooting development was also efficient in Woody Plant Media (WPM) supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin and 5.0 mg/L IAA.

Keywords

Acknowledgement

This research was conducted with the support of Korea Forest Service, Forest Science and Technology Development Project (No.: S120911L120110, S211213L030110).

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