융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

초임계 이산화탄소를 이용한 참당귀 유효 성분의 추출 및 생리활성 효능

Extraction of Active Compounds from Angelica gigas using Supercritical Carbon Dioxide and its Physiological Activity

  • 박수인 (을지대학교 대학원 시니어헬스케어학과) ;
  • 허수현 (을지대학교 대학원 시니어헬스케어학과) ;
  • 이진서 (을지대학교 대학원 시니어헬스케어학과) ;
  • 신문삼 (을지대학교 대학원 시니어헬스케어학과)
  • Park, Su In (Dept. of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji University) ;
  • Heo, Soo Hyeon (Dept. of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji University) ;
  • Lee, Jinseo (Dept. of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji University) ;
  • Shin, Moon Sam (Dept. of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji University)
  • 투고 : 2021.05.05
  • 심사 : 2021.06.20
  • 발행 : 2021.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 참당귀에서 유효 성분을 보다 효율적으로 추출하는 방법을 찾아 참당귀의 효능을 극대화하는 것을 목표로 하였다. 열수, 에탄올 및 초임계 이산화탄소 추출법으로 참당귀를 추출한 후, 데커신, 데커시놀 안젤레이트 함량 분석, 총 폴리페놀 함량 정량, 그리고 항산화, 미백, 항균에 대한 효능 평가를 진행하였다. 데커신, 데커시놀 안젤레이트의 함량은 초임계 이산화탄소 추출물에서 38.65%로 매우 높았고, 총 폴리페놀 함량은 열수 추출물, 에탄올 추출물, 초임계 이산화탄소 추출물 순으로 높았지만, 그 차이가 비교적 적었다. 항산화 효능은 총 폴리페놀 함량과 경향이 일치하였고, 항균 효능은 데커신, 데커시놀 안젤레이트 함량과 경향이 일치하였다. 본 연구를 통해 참당귀에서 유효 성분을 추출하기 위한 최적의 방법은 초임계 이산화탄소 추출법임을 확인하였다.

This study aimed to maximize the efficacy of Angelica gigas by finding a more effective way to extract active compounds from Angelica gigas. After extracting Angelica gigas by hydrothermal, ethanol, and supercritical carbon dioxide extraction methods, analysis of decursin and decursinol angelate content, quantification of total polyphenol content, and evaluation of efficacy of antioxidant, whitening, and antibacterial were conducted. The content of decursin and decursinol angelate was very high at 38.65% of the supercritical carbon dioxide extract, and the total polyphenol content was high in the order of hydrothermal extract, ethanol extract and supercritical carbon dioxide extract, but the difference was relatively small. The antioxidant effects were consistent with the total polyphenol content, and the antibacterial effects were consistent with the decursin and decursinol angelate content. In other words, through this study, we found that the optimal method for extracting active compounds from Angelica gigas is the supercritical carbon dioxide extraction method.

키워드

과제정보

This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded of the Ministry of Science & ICT (2017M3A9D8048416).

참고문헌

  1. C. G. Pereira, M. & A. A. Meireles. (2010). Supercritical fluid extraction of bioactive compounds: fundamentals, applications and economic perspectives. Food and Bioprocess Technology, 3(3), 340-372. DOI : 10.1007/s11947-009-0263-2
  2. M. E. Paulaitis, V. J. Krukonis, R. T. Kurnik, & R. C. Reid. (1983). Supercritical Fluid Extraction. Reviews in chemical engineering, 1(2), 179-250.
  3. J. L. Hedrick, L. J. Mulcahey, & L. T. Taylor. (1992). Supercritical fluid extraction. Microchimica Acta, 108(3), 115-132. DOI : 10.1007/BF01242421
  4. P. A. Uwineza & A. Wa kiewicz. (2020). Recent advances in supercritical fluid extraction of natural bioactive compounds from natural plant materials. Molecules, 25(17), 3847. DOI: 10.3390/molecules25173847
  5. R. Badal. (2002). Supercritical carbon dioxide extraction of lipids from raw and bioconverted rice bran. Master's thesis. Regional Engineering College, Jalandhar.
  6. E. Reverchon. (1997). Supercritical fluid extraction and fractionation of essential oils and related products. The Journal of Supercritical Fluids, 10(1), 1-37. DOI : 10.1016/S0896-8446(97)00014-4
  7. R. L. Mendes, B. P. Nobre, M. T. Cardoso, A. P. Pereira, & A. F. Palavra. (2003). Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae. Inorganica Chimica Acta, 356, 328-334. DOI : 10.1016/S0020-1693(03)00363-3
  8. S. Liu, F. Yang, C. Zhang, H. Ji, P. Hong, & C. Deng. (2009). Optimization of process parameters for supercritical carbon dioxide extraction of Passiflora seed oil by response surface methodology. The Journal of Supercritical Fluids, 48(1), 9-14. DOI : 10.1016/j.supflu.2008.09.013
  9. J. H. Kwon, M. S. Han, B. M. Lee, & Y. M. Lee. (2015). Effect of Angelica gigas extract powder on progress of osteoarthritis induced by monosodium iodoacetate in rats. Analytical Science and Technology, 28(1), 72-77. DOI : 10.5806/AST.2015.28.1.72
  10. S. K. Cho, A. M. Abd El-Aty, J. H. Choi, M. R. Kim, & J. H. Shim. (2007). Optimized conditions for the extraction of secondary volatile metabolites in Angelica roots by accelerated solvent extraction. Journal of Pharmaceutical and Biomedical Analysis, 44(5), 1154-1158. DOI : 10.1016/j.jpba.2007.03.011
  11. K. S. Ahn, W. S. Sim, H. M. Kim, S. B. Han, & I. H. Kim. (1996). Immunostimulating components from the root of Angelica gigas Nakai. Korean Journal of Pharmacognosy, 27(3), 254-261.
  12. O. Folin & W. Denis. (1912). On phosphotungstic-phosphomolybdic compounds as colorreagents. Journal of Biological Chemistry, 12(2), 239-243. DOI : 10.1016/S0021-9258(18)88697-5
  13. M. S. Blois. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. DOI : 10.1038/1811199a0
  14. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, & C. Rice-Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. DOI : 10.1016/S0891-5849(98)00315-3
  15. I. Kubo & I. Kinst-Hori. (1999). Flavonols from saffron flower: tyrosinase inhibitory activity and inhibition mechanism. Journal of agricultural and food chemistry, 47(10), 4121-4125. DOI : 10.1021/jf990201q
  16. M. O. Ko, H. J. Kang, J. H. Hwang, & K. W. Yang. (2018). Screening of the antibacterial effects by ethanol extracts from natural plant in Jeju against Propionibacterium acnes. Journal of the Society of Cosmetic Scientists of Korea, 44(1), 59-66. DOI : 10.15230/SCSK.2018.44.1.59
  17. J. H. Lee, Y. S. Choi, J. H. Kim, H. G. Jeong, D. H. Kim, M. Y. Yun, & G. Y. Song. (2006). A mass preparation method of (+)-decursinol from the roots of Angelica gigas. Yakhak Hoeji, 50(3), 172-176.
  18. K. W. Park et al. (2007). Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. Journal of the Korean Society of Food Science and Nutrition, 36(11), 1385-1390. DOI : 10.3746/jkfn.2007.36.11.1385
  19. A. K. Lim, J. O. Kim, M. J. Jung, H. K. Jung, J. H. Hong, & D. I. Kim. (2008). Functional biological activity of hot water and ethanol extracts from Taraxaci Herba. Journal of the Korean Society of Food Science and Nutrition, 37(10), 1231-1237. DOI : 10.3746/jkfn.2008.37.10.1231
  20. C. A. Rice-Evans, N. J. Miller, & G. Paganga. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine, 20(7), 933-956. DOI : 10.1016/0891-5849(95)02227-9
  21. Y. S. Lee, J. B. Choi, E. Y. Joo, & N. W. Kim. (2007). Antioxidative activities and tyrosinase inhibition of water extracts from Ailanthus altissima. Journal of the Korean Society of Food Science and Nutrition, 36(9), 1113-1119. DOI : 10.3746/jkfn.2007.36.9.1113