The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53

  • Zhu, Ri Zhe (Department of Biochemistry, Wonkwang University School of Medicine) ;
  • Li, Bing Si (Department of Biochemistry, Wonkwang University School of Medicine) ;
  • Gao, Shang Shang (Department of Biochemistry, Wonkwang University School of Medicine) ;
  • Seo, Jae Ho (Department of Biochemistry, Wonkwang University School of Medicine) ;
  • Choi, Byung-Min (Department of Biochemistry, Wonkwang University School of Medicine)
  • Received : 2020.09.07
  • Accepted : 2021.04.05
  • Published : 2021.07.01
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Abstract

Luteolin, a sort of flavonoid, has been reported to be involved in neuroprotective function via suppression of neuroinflammation. In this study, we investigated the protective effect of luteolin against oxidative stress-induced cellular senescence and its molecular mechanism using hydrogen peroxide (H2O2)-induced cellular senescence model in House Ear Institute-Organ of Corti 1 cells (HEI-OC1). Our results showed that luteolin attenuated senescent phenotypes including alterations of morphology, cell proliferation, senescence-associated 𝛽-galactosidase expression, DNA damage, as well as related molecules expression such as p53 and p21 in the oxidant challenged model. Interestingly, we found that luteolin induces expression of sirtuin 1 in dose- and time-dependent manners and it has protective role against H2O2-induced cellular senescence by upregulation of sirtuin 1 (SIRT1). In contrast, the inhibitory effect of luteolin on cellular senescence under oxidative stress was abolished by silencing of SIRT1. This study indicates that luteolin effectively protects against oxidative stress-induced cellular senescence through p53 and SIRT1. These results suggest that luteolin possesses therapeutic potentials against age-related hearing loss that are induced by oxidative stress.

Keywords

Acknowledgement

This study was supported by Wonkwang University in 2020.

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