한국균학회지 (The Korean Journal of Mycology)

  • 제49권1호
  • /
  • Pages.87-100
  • /
  • 2021
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

대전광역시 월평공원과 전북 격포해수욕장 주변 야생화와 토양들로부터 야생효모의 분리 및 국내 미기록 효모들의 특성과 생리 활성

Isolation of Wild Yeasts and Characterization of Physiological Functionalities of Unrecorded Wild Yeasts Obtained from Flowers and Soils of the Wolpyung Park, Daejeon City and Gykpo Beach, Buan, Jeollabuk-do in Korea

  • 장지은 (배재대학교 바이오의약학부) ;
  • 박선정 (배재대학교 바이오의약학부) ;
  • 이종수 (배재대학교 바이오의약학부)
  • Jang, Ji-Eun (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Park, Seon-Jeong (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 투고 : 2020.02.08
  • 심사 : 2021.03.08
  • 발행 : 2021.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

많은 사람들이 다녀가는 도시공원과 해변 환경의 효모 특성을 알아보기 위해 대전광역시 월평공원 갑천 누리길과 내원사 주변의 야생화와 토양 75점에서 모두 88균주의 야생 효모들을 분리, 동정하였다. 또한 전북 부안의 격포해수욕장과 격포항 주변 야생화와 토양 35점에서 30균주의 야생 효모들을 분리하였다. 이들 분리 야생 효모들 중 A. porosum ASCM32-1, A. scarabaeorum WP49-1, C. athensensis WP4-90-3, C. berkhoutiae WP34-2, C. bracarensis WP35-1, Cy. amylophila GP19-1, K. gamospora WP9-1, M. carpophila BN-17-1, T. namnaoensis WP4-89-1, V. taibaiensis WP13-2 등 10종의 국내 미기록종 후보 균주들을 선별하여 균학적 특성을 확인하였다. 이들 미기록 효모들은 주로 구형~타원형으로 모두 출아방식으로 영양 증식을 하였고 V. taibaiensis WP13-2와 K. gamospora WP9-1, C. athensensis WP4-90-3 외 7종의 미기록 효모들은 자낭포자를 형성하지 않은 무포자 효모들이었으며A. porosum ASCM32-1만이 의균사를 생성하였다. 대부분의 균주들은 YPD 배지와 YM 배지에서생육하였고V. taibaiensis WP13-2와K. gamospora WP9-1, T. namnaoensis WP4-89-1 균주들은 15% NaCl을 함유한 YPD 배지에서 생육하는 호염성 효모들이었고, Cy. amylophila GP19-1 균주는 40℃에서도 생육하였다. C. athensensis WP4-90-3 균주 등 5균주들이 10% 에탄올 내성을 보였고C. athensensis WP4-90-3 균주는 Li2+ 등 많은 중금속들에 대해서 내성을 보였다. 국내 미기록 효모들과 비병원성 야생 효모 균주 무세포추출물들의 β-glucuronidase 저해 활성은 C. oleophila WP5-19-1의 무세포 추출물이 49.0%로 가장 높았고 미기록 효모인 K. gamospora WP9-1의 무세포 추출물도 27.7%를 보였다. 호중구 elastase 저해 활성은 W. anomalus HO9-2가 38.4%로 우수하여 건강 소재로 생산에 매우 유용한 균주로 활용될 것으로 기대된다.

This study aimed to isolate wild yeasts obtained from flowers and soil of the Wolpyung park, Daejeon city and Gykpo beach, Buan, Jeollabuk-do in Korea, and to further characterize previously unrecorded wild yeast strains. In total, 88 strains of 62 different species of wild yeasts were isolated from 75 samples obtained from the Wolpyung park. Among these, six strains of Trichosporon moniliiforme and four strains each of Papiliotrema flavescens and Candida melibiosica were isolated. Additionally, 39 strains of 30 different species of wild yeasts were isolated from 35 samples collected from the Gykpo beach. Among the 127 isolated wild yeast strains, 10 strains, including Apiotrichum porosum ASCM32-1, were previously unrecorded. All the 10 previously unrecorded yeasts were oval or global in shape, and three strains, including Candida athensensis WP4-90-3, formed spores. Three strains, including Vishniacozyma taibaiensis WP13-2, were halophilic yeasts which grew in 15% NaCl-containing YPD(yeast extract-peptone-dextrose) medium. Five strains, including C. athensensis WP4-90-3, showed 15% ethanol resistance. Cell-free extracts from Candida oleophila WP5-19-1 and Wickerhamomyces anomalus HO9-2 showed the highest β-glucuronidase inhibitory activity (49.0%) and neutrophil elastase inhibitory activity (38.4%), respectively.

키워드

과제정보

This research was supported by the project(NIBR202002116) on the survey of Korean indigenous species of the National Institute of Biological Resources under the Ministry of Environment, Republic of Korea.

참고문헌

  1. Lee JS, Lee SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.
  2. Lee JS, Choi YJ, Kwon SJ, Yoo JY, Chung DH. Screening and characterization of osmotolerant and gas-producing yeasts from traditional Doenjang and Kochujang. Food and Biotechnol 1996;5:54-8.
  3. Min JH, Ryu JJ, Kim HK, Lee JS. Isolataion and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Baekamsan of Korea. Kor J Mycol 2013;41:47-50. https://doi.org/10.4489/KJM.2013.41.1.47
  4. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  5. Hyun SH, Lee JK, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea Kor J Mycol 2014;42:21-7. https://doi.org/10.4489/KJM.2014.42.1.21
  6. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  7. Hyun SH, Min JH, Kim SA, Lee JS, Kim HK. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82. https://doi.org/10.4489/KJM.2014.42.2.178
  8. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  9. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  10. Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, Republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiol 2015;43:266-71. https://doi.org/10.5941/MYCO.2015.43.3.266
  11. Han SM, Han JW, Bae SM, Park WJ, Lee JS. Isolation and identification of wild yeasts from soils of paddy fields in Daejeon metropolitan city and Chungcheongnam-do, Korea. Kor J Mycol 2016;44:1-7. https://doi.org/10.4489/KJM.2016.44.1.1
  12. Han SM, Lee JS. Isolation and identification of wild yeasts from soils of an herb park in Seoul metropolitan city and characteristics of unrecorded yeasts. Kor J Mycol 2016;44:108-115. https://doi.org/10.4489/KJM.2016.44.2.108
  13. Han SM, Lee SY, Kim HK, Lee JS. Characterization of wild yeasts Isolated from leaves obtained from Mt. Daedun and Mt. Chilgap, Korea. Kor J Mycol 2017;45:31-42. https://doi.org/10.4489/KJM.20170004
  14. Han SM, Lee JS. Characterization of unrecorded yeasts Isolated from leaves of trees of Oknyeobong peak and Yeonjasan mountain in Daejeon, Korea. Kor J Mycol 2017;45:23-30. https://doi.org/10.4489/KJM.20170003
  15. Han SM, Kim HK, Lee HB, Lee JS. Isolation and identification of wild yeasts from freshwaters and soils of Nakdong and Yeongsan river, Korea, with characterization of two unrecorded yeasts. Kor J Mycol 2016;44:350-54. https://doi.org/10.4489/KJM.2016.44.4.350
  16. Han SM, Lee SY, Kim HK, Lee JS. Isolation and diversity of wild yeasts from the waters and bank soils of Daejeoncheon, Gapcheon, and Yedeungcheon in Daejeon metropolitan city, Korea. Kor J Mycol 2017;45:259-69. https://doi.org/10.4489/KJM.20170034
  17. Han SM, Kim JY, Lee HB, Kim HK, Lee JS. Isolation and characterization of wild yeasts from water and riverside soils of Geumgang midstream in Gongju City, Korea. Kor J Mycol 2018;46:98-104. https://doi.org/10.4489/KJM.20180013
  18. Han SM, Lee SY, Lee JS. Isolation of wild yeasts from humus-rich soil in city park of Daejeon metropolitan city, Korea, and characterization of the unrecorded wild yeasts. Kor J Mycol 2018;46;75-82. https://doi.org/10.4489/KJM.20180010
  19. Kim JY, Han SM, Lee JS. Isolation and tyrosinase inhibitory activity of wild yeasts obtained from soil in the fields of medicinal plants, Ginseng and Korean angelica. Kor J Mycol 2018;46:205-11. https://doi.org/10.4489/KJM.20180036
  20. Han SM, Kim JY, Kim CM, Lee JS. Characteristics of unrecorded wild yeasts obtained from the soil of spices plant fields and its physiological functionality. Kor J Mycol 2019;47;75-81. https://doi.org/10.4489/KJM.20190009
  21. Han SM, Kim JY, Lee JS. Isolation of wild yeasts from the water and riverside soil of Geumgang midstream in Sejong city, Korea, and characterization of unrecorded wild yeasts. Kor J Mycol 2019;47;75-81. https://doi.org/10.4489/KJM.20190009
  22. Kim HK, Kim JY, Han SM, Kim CM, Lee JS. Microbiological characteristics and physiological functionalities of unrecorded wild yeast strains in the soils of Hajodae and Gyungpodae beaches in Korea. Kor J Mycol 2019;47:249-58. https://doi.org/10.4489/KJM.20190029
  23. Han SM, Hyun SH, Kim NM, Lee JS. Antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase of yeasts from wild flowers in Korea. Kor J Mycol 2015;43:99-103. https://doi.org/10.4489/KJM.2015.43.2.99
  24. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Microbiological characteristics and physiological functionality of new records of yeasts from wild flowers in Yokjido, Korea. Mycobiol. 2014;42:198-202. https://doi.org/10.5941/myco.2014.42.2.198
  25. Han SM, Lee SY, Lee HB, Lee JS. Isolation of wild yeasts from soils of reed fields in Seocheon-gun county, Chungcheongnam-do, south Korea, and characterization of unrecorded yeasts. Kor J Mycol 2017;45:234-50. https://doi.org/10.4489/KJM.20170028
  26. Kim JY, Han SM, Park SJ, Jang JE, Lee JS. Isolation and characterization of unrecorded wild yeasts obtained from soils of spice fields and mountains. Kor J Mycol 2020;48:151-160. https://doi.org/10.4489/KJM.20200016
  27. Han SM, Park DJ, Kim JY, Lim HJ, Lee HB, Lee JS. Isolation of wild yeasts obtained from waters and soils of riversides in Sangjubo of Nakdong river and Daechung dam of Geumgang midstream, Korea and characterization of unrecorded wild yeasts. Kor J Mycol 2020;48:237-49. https://doi.org/10.4489/KJM.20200025
  28. Kim JS, Lee M, Kim JY, Heo J, Kwon SW, Yun BS, Kim SJ. Distribution and species diversity of wild yeasts isolated from flowers in Korea. Kor J Mycol 2020;48:475-84. https://doi.org/10.4489/KJM.20200045
  29. Jeon YJ, Park S, Hwang H, Park YH, Cheon W, Goh J, Chung N, Mun HY. Seven yeast strains isolated from freshwaters for the first record in Korea. Kor J Mycol 2020;48:523-31. https://doi.org/10.4489/KJM.20200052
  30. Lee IS, Ryoo IJ, Kwon KY, Ahn JS, Yoo ID. Pleurone, a novel human neutrophil elastase inhibitor from the fruiting bodies of the mushroom Pleurotus eryngii var. ferulae. J. Antibiotics 2011;64:587-9. https://doi.org/10.1038/ja.2011.47
  31. Oh EH, Park JM., Kim SH, Song IG, Han NS, Yoon HS. Biological activities of Phellinus linteus mycelium culture with Cassiae semen extract on β-glucuronidase inhibitory activity. Kor J Food Nutr 2012;25:620-8. https://doi.org/10.9799/ksfan.2012.25.3.620
  32. Rhee YK, Kim DH, Han MJ. Inhibitory effect of Zizyphi fructus on β-glucuronidase and tryptophanase of human intestinal bacteria. Kor J Food Sci Technol 1998;30:199-205.
  33. Kim DH, Choi HJ, Bae EA, Han MJ, Park SY. Effect of artificially cultured Phellinus linteus on harmful intestinal bacterial enzymes and rat intestinal α-glucosidases. J Fd Hyg Safety 1998;13:20-3.
  34. Han MJ, Bae EA, Rhee YK, Kim DH. Effect of mushrooms on the growth of intestinal lactic acid bacteria. Kor J Food Sci Technol, 1996;28:947-52.
  35. Li ZP, Kim JY, Ban YJ, Park KH. Human neutrophil elastase (HNE) inhibitory polyprenylated acylphloroglucinols from the flowers of Hypericum ascyron. Bioorg Chem 2019;90:103075. https://doi.org/10.1016/j.bioorg.2019.103075