Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus

  • Kim, Ju-Won (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Cho, Ja Young (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Chun, Won-Kyong (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Dong-Gyun (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Nam, Bo-Hye (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Nho, Eun-Soo (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kim, Young-Ok (Biotechnology Research Division, National Institute of Fisheries Science) ;
  • Kong, Hee Jeong (Biotechnology Research Division, National Institute of Fisheries Science)
  • Received : 2021.08.03
  • Accepted : 2021.09.25
  • Published : 2021.11.30
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Abstract

In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, the FGBC8 cells were incubated with the several mitogens such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly I:C), flagellin, and interferon (IFN)-γ. After incubation, the expression of immune-related gene was observed in FGBC8 cells through the quantitative real-time PCR. Our results indicate that FGBC8 cells will serve as a valuable research tool for investigating host-pathogen interactions as well as cell biotechnological applications.

Keywords

Acknowledgement

This research was supported by a research project (R2021025) managed by the National Institute of Fisheries Science (NIFS), Korea.

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