융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
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매염제인 ZnSO4의 피부독성에 대한 멍석딸기 추출물의 항산화 및 미백효과

Antioxidative and Whitening Effects of Rubus parvifolius L. Extract on Dermal Cytotoxicity of ZnSO4, Mordant

  • 손영우 (원광대학교 의과대학 산본병원) ;
  • 유선미 (원광보건대학교 미용피부화장품과)
  • Sohn, Young-Woo (Divion of Medicine School, Sanbon Hospital, Wonkwang University) ;
  • Yoo, Sun-Mi (Division of Beauty&Cosmetics, Wonkwang Health Science University)
  • 투고 : 2021.03.17
  • 심사 : 2021.05.20
  • 발행 : 2021.05.28
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초록

본 연구는 매염제인 황산아연(ZnSO4)의 피부독성을 배양 피부세포주인 SK-MEL-3 세포를 재료로 산화적 손상측면에서의 조사와 함께 ZnSO4의 독성에 대한 멍석딸기(RP)의 영향을 항산화와 미백효과 측면에서 알아보았다. 본 실험을 위하여, 세포생존율, DPPH-라디칼 소거능 및 melanin합성 저해능을 분석하였다. 본 연구에서 ZnSO4는 농도 의존적으로 세포생존율을 유의하게 감소시켰으며, XTT50값이 173.3 uM로 중간 독성으로 나타났다. 또한, 항산화제의 일종인 ascorbic acid는 ZnSO4에 의하여 손상된 세포생존율을 유의하게 증가시켰다. 한편, ZnSO4의 독성에 대한 RP 추출물의 영향에서, RP 추출물 처리는 유의한 세포생존율의 증가와 함께 DPPH-라디칼 소거능과 melanin합성 저해능을 통하여 항산화와 미백효과를 나타냈다. 결론적으로, RP 추출물과 같은 천연성분은 향후 항산화제와 미백제로서의 대체물질 개발에 있어 활용적 가치가 클 것으로 생각된다.

This study was done to evaluate the dermatoxicity of zinc sulfate (ZnSO4) and the protective effect of Rubus parvifolius L. (RP) extract on cytotoxicity of ZnSO4, mordant in cultured SK-MEL-3 cells. For this study, it was done an antioxidative effect as DPPH-radical scavenging ability as well as the diminutive ability of total melanin with cell viability. ZnSO4 significantly decreased cell viability in dose-dependently, and it was mid-toxic. The ascorbic acid significantly increased cell viability damaged by ZnSO4-induced cytotoxicity. In the protective effect of RP extract on ZnSO4-induced cytotoxicity, RP extract significantly increased cell viability compared with ZnSO4-treated group, and also it showed both the DPPH-radical scavenging ability and the decrease of total amount of melanin. From these findings, the cytotoxicity of ZnSO4 is correlated with oxidative stress, and also RP extract effectively protected ZnSO4-induced cytotoxicity via antioxidative effect such as DPPH-radical scavenging ability with the whitening effect by the decrement of total amount of melanin. Conclusively, the natural ingredients like RP extract may be a useful agent for the improvement of antioxidative and whitening effects

키워드

과제정보

This paper was supported by Wonkwang University in 2020

참고문헌

  1. E. K. Hwang, Y. H. Lee & H. D. Kim. (2008). Dyeing, fastness, and deodorizing properties of cotton, silk, and wool fabrics dyed with gardenia coffee sludge,Cassia tora L., and pomegranate extract. Fibers and Polymers, 9(3), 334-340. https://doi.org/10.1007/s12221-008-0054-9
  2. Miao J, Jia Z, Lu HB, Habibi D & Zhang LC.(2014). Heterogeneous photocatalytic degradation of mordant black 11 with ZnO nanoparticles under UV-vis light. Journal of the Taiwan Institute of Chemical Engineers, 45(4), 1636-1641. https://doi.org/10.1016/j.jtice.2013.11.007
  3. S. H. Lee, I. J. Jung & H. S. Jang. (2018). The antioxidative effect of Eclipta prostrate L. extract on cultured NIH3T3 fibrobalsts injured by manganese-induced cytotoxicity. Biomedical Science Letters, 24(4), 1-8. https://doi.org/10.15616/BSL.2018.24.1.1
  4. J. Y. Jung, H. S. Jang & Y. M. Seo. (2017). Protective effect of Ajuga multiflora BUNGE extract on lead toxicity of environmental pollutant. Journal of People Plant Environment, 20(4), 341-350. https://doi.org/10.11628/ksppe.2017.20.4.341
  5. J. S. Shin, O. K. Sim, J. C. Lee, H. J. Cho, E. Y. Kim & K. S. Lee. (2005). Plant regeneration via Multiful Shoots Formation from Sucher Explants of Rubus fruticosus L. Korean Journal of Plant Resources, 18(3), 456-451.
  6. Do JC, Son KH & Kang SS.(1988). Studies on the constituents of the roots of Rubus parvifolius(I). Biopharmacology, 19(3), 170-173.
  7. N. Lavid, A. Schwartz, O. Yarden & E. Tel-Or. (2001). The involvement of polyphenols and peroxidase activities in heavy-metal accumulation by epidermal glands of the waterlily (Nymphacaceae). Planta, 212, 323-331. https://doi.org/10.1007/s004250000400
  8. K. W. Kim, C. K. Baek, Y. U. Chang, E. J. Kim, Y. S. Kwon, H. J. Kim & H. R. Shon. (2005). Screeing of antibacterial agent against streptococcus mutans from natural and medicinal plants. Bioscience, 15(5), 715-725.
  9. A.O.A.C.(2005). Official method of analysis. (12th). A.O.A.C., Washington D.C. 127-130.
  10. M. I. Nieva Moreno, M. I. Isla, A. R. Sampietro & M. A. Vattuone. (2000). Comparison of the three radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethmopharmacoogy, 71, 109-114. DOI : 10.1016/S0378-8741(99)00189-0
  11. M. S. Blois. (1958). Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  12. J. Hosei, E. Abe, T. Suda & T. Kuroki. (1985). Regulation of melanin synthesis of B16 mouse melanoma cells by 1 a-25-dihydroxyvitamin D3 and retinoic acid. Cancer Research, 45, 1474-1482.
  13. J. Y. Jung, Y. H. Oh, S. H. Park, M. Y. Yoon, A. J. Pyo & S. K. Kim. (2014). Antioxidative and whitening effects of Houttuynia cordata L. extract on lead acetate on hair dye component. Journal of Investigative Cosmetology, 10(2), 99-105.
  14. E. Borenfreund & J. A. Puerner. (1985). A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90). Journal of Tissue Culture Methods, 9, 7-9. https://doi.org/10.1007/BF01666038
  15. S. T. Yang, J. H. Liu, J. Wang, Y. Yuan, A. Cao, H. Wang & Y. Zhao. (2010). Cytotoxicity of zinc oxide nanoparticles: importance of microenvironment. Journal of Nanoscience and Nanotechnology, 10(12), 8638-8645. https://doi.org/10.1166/jnn.2010.2491
  16. S. Tang, V. Allagadda, H. Chibli, J. L. Nadeau & G. D. Mayer. .(2013). Comparison of cytotoxicity and expression of metal regulatory genes in Zebrafish (Danio rerio) liver cells exposed to cadmium sulfate, zinc sulfate and quantum dots. Metallomics, 5(10), 1411-1422. https://doi.org/10.1039/c3mt20234h
  17. Y. L. Oh, Y. R. Choi, B. S. Chang & I. J. Jung. (2012). Antioxidative effect of Portulaca oleracea L. extract on allergic contact dermatitis-induced agent, copper in cultured human skin fibroblasts, Journal of Investigative Cosmetology, 8(4), 243-249. https://doi.org/10.15810/jic.2012.8.4.004
  18. A. J. Pyo, M. Y. Yoon & H. O. Yang. (2013). Cytotoxicity and protective effects of Smilax china L. extract on melanogenesis by FeSO4, an autooxidant of melaninformation. Korean Journal of Aesthetics & Cosmetology. 11, 77-83.
  19. H. J. Park, J. W. Nam, S. C. Rim & W. B. Kim. (2016). Chemical characterization and utilization of 19 alpha-hydroxyursane-type triterpenoidsin Rubus species. Korean Journal of Plant Resources. 19(5), 563-572.
  20. J. R. Kang, J. Y. Lee & W. K. Whang. (2007). Antioxidant activity and tyrosinase inhibitory activity of Elscholtziae splendense. Journal of Korean Scociety Cosmetology, 13, 163-170.