Advances in nano research

  • 제10권5호
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  • Pages.461-470
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  • 2021
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  • 2287-237X(pISSN)
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  • 2287-2388(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

DOI QR Code

Synthesis and radiolabeling of PEGylated dendrimer-G2-Gemifloxacin with 99mTc to Biodistribution study in rabbit

  • Mohtavinejad, Naser (Department of Radiopharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences) ;
  • Dolatshahi, Shaya (Pharm, D. Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Amanlou, Massoud (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Ardestani, Mehdi Shafiee (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Asadi, Mehdi (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Pormohammad, Ali (Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences)
  • 투고 : 2020.06.07
  • 심사 : 2021.01.17
  • 발행 : 2021.05.25
⟨ 이전 논문 다음 논문 ⟩

초록

Infection is one of the major mortality causes throughout the globe. Nuclear medicine plays an important role in diagnosis of deep infections such as osteomyelitis, arthritis infection, heart valve and heart prosthesis infections. Techniques such as labeled leukocytes are sensitive and selective for tracking the inflammations but they are not suitable for differentiating infection from inflammation. Anionic linear-globular dendrimer-G2 was synthesized then conjugation to gemifloxacin antibiotic. The structures were identified by FT-IR, 1H-NMR, C-NMR, LC-MS and DLS. The toxicity of gemifloxacin and dendrimer-gemifloxacin complex was compared by MTT test. Dendrimer-G2-gemifloxacin was labeled by Technetium-99m and its in-vitro stability and radiochemical purity were investigated. In-vivo biodistribution and SPECT imaging were studied in a rabbit model. Identify and verify the structure of the each object was confirmed by FT-IR, 1H-NMR, C-NMR and LC-MS, also, the size and charge of this compound were 128 nm and -3/68 mv respectively. MTT test showed less toxicity of the dendrimer-G2-gemifloxacin than free gemifluxacin (P < 0.001). Radiochemical yield was > %98. Human serum stability was 84% up to 24 h. Biodistribution study at 50 min, 24 and 48 h showed that the complex is significantly absorbed by the intestine and accumulation in the lungs and affects them, finally excreted through the kidneys, biodistribution results are consistent with results from full image means of SPECT/CT technique.

키워드

과제정보

The authors are grateful to the Research Council of Tehran University of Medical Sciences, Tehran, Iran for financial supports.

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