Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Oscillatory behavior of microglial cells

미세아교세포의 진동 거동의 연구

  • Park, Eunyoung (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Cho, Youngbin (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Ko, Ung Hyun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Park, Jin-Sung (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Shin, Jennifer H. (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
  • Received : 2021.01.27
  • Accepted : 2021.04.08
  • Published : 2021.04.30
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Abstract

Cells regulate their shapes and motility by sensing the cues from the internal and external microenvironment. Under different circumstances, microglia, the brain resident immune cells, undergo dynamic phenotypic changes, one of which is a remarkable periodic oscillatory migration in vitro. However, very little is known about the kinematic and dynamic perspectives of this oscillatory behavior. In this study, we tracked the changes in cell morphology and nuclear displacement, and visualized the forces using traction force microscopy (TFM). By correlation analyses, we confirmed that the lamellipodia formation preceded the nuclear translocation. Moreover, traction, developed following lamellipodia formation, was found to be localized and fluctuated at two ends of the oscillating cells. Taken together, our results imply that oscillatory microglial cells feature a viscoelastic migration, which will contribute to the field of cell mechanics.

Keywords

Acknowledgement

이 논문은 KAIST에서 지원하는 2021년도 글로벌 특이점 연구사업으로 수행된 연구임.

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