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DNA Barcoding of a Worldwide Colonial Ascidian, Diplosoma listerianum (Ascidiacea: Aplousobranchia: Didemnidae), from East Sea, Korea

  • Lee, Taekjun (Department of Animal Biotechnology and Resource, Sahmyook University) ;
  • Shin, Sook (Department of Animal Biotechnology and Resource, Sahmyook University)
  • Received : 2020.09.22
  • Accepted : 2021.04.06
  • Published : 2021.04.30

Abstract

Diplosoma listerianum (Milne Edwards, 1841) is a globally distributed species that encompasses the existence of multiple cryptic species. In this study, partial sequences of cytochrome c oxidase subunit I (COI) from D. listerianum in South Korea were determined and compared with COI sequences of known D. listerianum and other Diplosoma species retrieved from GenBank. The results showed that Korean D. listerianum matched with clade A of D. listerianum, which is a group distributed globally. These new DNA barcodes for Korean D. listerianum may be useful in the identification of colonial ascidians, which is difficult to morphological identify.

Keywords

Acknowledgement

This study was the project titled 'Improvement of management strategies on marine disturbing and harmful organisms (No. 20190518)' funded by the Ministry of Oceans and Fisheries, Korea.

References

  1. Bock DG, MacIsaac HJ, Cristescu ME, 2012. Multilocus genetic analyses differentiate between widespread and spatially restricted cryptic species in a model ascidian. Proceedings of the Royal Society of London, Series B, 279:2377-2385. https://doi.org/10.1098/rspb.2011.2610
  2. Brunetti R, Gissi C, Pennati R, Caicci F, Gasparini F, Manni L, 2015. Morphological evidence that the molecularly determined Ciona intestinalis type A and type B are different species: Ciona robusta and Ciona intestinalis. Journal of Zoological Systematics and Evolutionary Research, 53:186-193. https://doi.org/10.1111/jzs.12101
  3. Brunetti R, Manni L, Mastrototaro F, Gissi C, Gasparini F, 2017. Fixation, description and DNA barcode of a neotype for Botryllus schlosseri (Pallas, 1766) (Tunicata, Ascidiacea). Zootaxa, 4353:29-50. https://doi.org/10.11646/zootaxa.4353.1.2
  4. Darriba D, Taboada GL, Doallo R, Posada D, 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9:772. https://doi.org/10.1038/nmeth.2109
  5. Da Silva Oliveira FA, Michonneau F, da Cruz Lotufo TM, 2017. Molecular phylogeny of Didemnidae (Ascidiacea: Tunicata). Zoological Journal of the Linnean Society, 180:603-612. https://doi.org/10.1093/zoolinnean/zlw002
  6. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O, 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59:307-321. https://doi.org/10.1093/sysbio/syq010
  7. Kimura M, 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16:111-120. https://doi.org/10.1007/BF01731581
  8. Kott P, 2001. The Australian Ascidiacea Part 4, Aplousobranchia (3), Didemnidae. Memoirs of the Queensland Museum, 47:1-407.
  9. Kumar S, Stecher G, Tamura K, 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  10. Lambert G, 2007. Invasive sea squirts: a growing global problem. Journal of Experimental Marine Biology and Ecology, 342:3-4. https://doi.org/10.1016/j.jembe.2006.10.009
  11. Lopez-Legentil S, Legentil ML, Erwin PM, Turon X, 2015. Harbor networks as introduction gateways: contrasting distribution patterns of native and introduced ascidians. Biological Invasions, 17:1623-1638. https://doi.org/10.1007/s10530-014-0821-z
  12. Perez-Portela R, Arranz V, Rius M, Turon X, 2013. Cryptic speciation or global spread? The case of a cosmopolitan marine invertebrate with limited dispersal capabilities. Scientific Reports, 3:3197. https://doi.org/10.1038/srep03197
  13. Rho BJ, Huh MK, 1984. A systematic study on the Ascidians in Korea. Journal of Korean Research Institute for Better Living, Ewha Womans University, 33:99-136.
  14. Rius M, Teske PR, 2013. Cryptic diversity in coastal Australasia: a morphological and mitonuclear genetic analysis of habitat-forming sibling species. Zoological Journal of the Linnean Society, 168:597-611. https://doi.org/10.1111/zoj.12036
  15. Shenkar N, Gittenberger A, Lambert G, Rius M, Moreira da Rocha R, Swalla BJ, Turon X, 2021. Ascidiacea World Database. Botrylloides Milne Edwards, 1841 [Internet]. World Register of Marine Species, Accessed 29 Mar 2021, .
  16. Smith KF, Abbott CL, Saito Y, Fidler AE, 2015. Comparison of whole mitochondrial genome sequences from two clades of the invasive ascidian, Didemnum vexillum. Marine Genomics, 19:75-83. https://doi.org/10.1016/j.margen.2014.11.007
  17. Tarjuelo I, Posada D, Crandall KA, Pascual M, Turon X, 2004. Phylogeography and speciation of colour morphs in the colonial ascidian Pseudodistoma crucigaster. Molecular Ecology, 13:3125-3136. https://doi.org/10.1111/j.1365-294X.2004.02306.x
  18. Tokioka T, 1970. Ascidians from Mindoro Island, the Philippines. Publications of the Seto Marine Biological Laboratory, 18:75-107. https://doi.org/10.5134/175626
  19. Turon X, Tarjuelo I, Duran S, Pascual M, 2003. Characterising invasion processes with genetic data: an Atlantic clade of Clavelina lepadiformis (Ascidiacea) introduced into Mediterranean harbours. Hydrobiologia, 503:29-35. https://doi.org/10.1023/B:HYDR.0000008481.10705.c2