한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제25권3호
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  • Pages.173-183
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  • 2021
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Generation of Miniaturized Ovaries by In Vitro Culture from Mouse Gonads

  • Jang, Si Won (Dept. of Agricultural Convergence Technology, Jeonbuk National University) ;
  • Choi, Hyun Woo (Dept. of Agricultural Convergence Technology, Jeonbuk National University)
  • 투고 : 2021.05.16
  • 심사 : 2021.08.17
  • 발행 : 2021.09.30
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초록

The incidence of infertility among individuals of reproductive age has been growing due to genetic and environmental factors, and considerable research efforts are focused on solving this issue. Ovarian development is an overly complex process in the body, involving the interaction between primordial germ cells and gonad somatic cells. However, follicles located in the center of the in vitro ovary are poorly formed owing to ovarian complexity, nutrient deficiency, and signaling deficiency. In the present study, we optimized methods for dissociating gonads and culture conditions for the in vitro generation of miniaturized ovaries. The gonads from embryos were dissociated into cell masses and cultured on a Transwell-COL membrane for 3-5 weeks. Approximately 12 follicles were present per in vitro ovary. We observed that miniaturized ovaries successfully matured to MII oocytes in vitro from 150 to 100 ㎛ gonad masses. This method will be useful for investigating follicle development and oocyte production.

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과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP; Ministry of Science, ICT & Future Planning) (NRF-2017R1C1B5077043).

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