한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제25권4호
  • /
  • Pages.313-319
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  • 2021
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Lin28 and Imp are Required for Stability of Bowl Transcripts in Hub Cells of the Drosophila Testis

  • To, Van (School of Biological Sciences and Technology, Chonnam National University) ;
  • Kim, Hyun Ju (School of Biological Sciences and Technology, Chonnam National University) ;
  • Jang, Wijeong (School of Biological Sciences and Technology, Chonnam National University) ;
  • Sreejith, Perinthottathil (School of Biological Sciences and Technology, Chonnam National University) ;
  • Kim, Changsoo (School of Biological Sciences and Technology, Chonnam National University)
  • 투고 : 2021.10.15
  • 심사 : 2021.12.15
  • 발행 : 2021.12.31
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초록

Hub cells comprise a niche for germline stem cells and cyst stem cells in the Drosophila testis. Hub cells arise from common somatic gonadal precursors in embryos, but the mechanism of their specification is still poorly understood. Here we find that RNA binding proteins Lin28 and Imp mediate transcript stability of Bowl, a known hub specification factor; Bowl transcripts were reduced in the testis of Lin28 and Imp mutants, and also when RNA-mediated interference against Lin28 or Imp was expressed in hub cells. In tissue culture Luciferase assays involving the Bowl 3'UTR, stability of Luc reporter transcripts depended on the Bowl 3'UTR and required Lin28 and Imp. Our findings suggest that proper Bowl function during hub cell specification requires Lin28 and Imp in the testis hub cells.

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과제정보

This work was supported by grants from the National Research Foundation of Korea (NRF-2021R1A2C1010334 to CK, NRF-2020R1I1A1A01074292 to WJ, NRF-2017R1A6A3A11032327 to WJ). We thank the Bloomington Drosophila Stock Center (BDSC) and Vienna Drosophila RNAi Center (VDRC) for flies, and the Drosophila Genetic Resource Center (DGRC) for Bowl cDNA. We are grateful to F. Michon for sharing Lin28df30, T. Guy for Upd-Gal4, and N Sokol for Lin28Δ1.

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