DOI QR코드

DOI QR Code

Antioxidant Effect of Hot Water Extracts from 3 Types Indonesia Plants (Hibiscus Petals, Moringa Oleifera Gymnosperm, and Nipa Fruticans Wurmb)

인도네시아 식물 3종(히비스커스 꽃잎, 모링가 겉씨, 해죽순) 열수추출물의 항산화 효과

  • Choi, Ji-Hye (Department of Medical Laboratory Science, College of Health Science, Dankuk University) ;
  • Hwang, Jin-Woo (Department of Medical Laboratory Science, College of Health Science, Dankuk University) ;
  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankuk University) ;
  • Heo, Su-Hak (Department of Biomedical Chemistry, Konkuk Univ. Glocal Campus) ;
  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankuk University)
  • 최지혜 (단국대학교 임상병리학과 임상분자생물학전공) ;
  • 황진우 (단국대학교 임상병리학과 임상분자생물학전공) ;
  • 이성규 (단국대학교 임상병리학과 임상분자생물학전공) ;
  • 허수학 (건국대학교 글로컬캠퍼스 의생명화학전공) ;
  • 강현 (단국대학교 임상병리학과 임상분자생물학전공)
  • Received : 2020.11.11
  • Accepted : 2020.12.14
  • Published : 2021.03.31

Abstract

Purpose: This study investigated the antioxidant activities of water extracts from Hibiscus petals, Moringa oleifera gymnosperm, and Nipa fruticans wurmb. Also, the possibility of their use as a functional cosmetic material and food were searched. Methods: We extracted Hibiscus petals, M. oleifera gymnosperm, and N. fruticans wurmb with water. And then, we measured the content of total polyphenols and flavonoids and the ability to scavenging free groups of ABTS and DPPH to study the antioxidant function. The toxicity of samples evaluated by measuring cell viability. Results: The polyphenol content of the water extract of N. fruticans wurmb was 109 ㎍/mg, which was significantly higher than that of Hibiscus petals (13 ㎍/mg) and M. oleifera gymnosperm (19 ㎍/mg). Radical scavenging ability was also excellent in N. fruticans wurmb, and the cytotoxicity test results of the samples were similar. Conclusions: The water extracts showed antioxidant activity to use for cosmetic materials or natural healing foodstuff.

목적: 본 연구의 목적은 인도네시아 원료 중히비스커스 꽃잎, 모링가 겉씨, 해죽순(차용)을 열수로 추출하여 항산화 능을 비교하여 어떠한 시료가 효능이 높은지 조사하고, 또한 기능성 화장품 소재나 기능성 식품으로의 활용 가능성에 관해서도 연구하는 것이었다. 방법: 히비스커스 꽃잎, 모링가 겉씨, 해죽순 추출물을 열수 추출하여 polyphenol, flavonoid의 함유량 및 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS)와 1,1-diphenyl-2-picryl hydrazyl(DPPH) radical의 소거 능력을 측정하여 항산화 기능을 검사하였고, 세포 생존율을 측정하여 시료들의 독성을 평가하였다. 결과: 해죽순 열수 추출물의 polyphenol 함유량은 109 ㎍/mg으로 히비스커스 꽃잎(13 ㎍/mg)과 모링가 겉씨(19 ㎍/mg) 보다 월등히 높았으며, flavonoid 함유량은 다른 모링가와 히비스커스보다 높게 나타났다. ABTS와 DPPH radical 소거능 또한 해죽순이 높았으며, 상기시료들의 세포독성 실험결과는 비슷하였다. 결론: 위의 히비스커스 꽃잎, 모링가 겉씨, 해죽순의 열수 추출한 결과물이 항산화 작용이 높고 독성이 거의 없어서 화장품 물질이나 자연유적 식품으로 사용할 수 있다고 판단하였다.

Keywords

Acknowledgement

본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 보건의료기술연구개발사업 지원에 의하여 이루어진 것임 (과제고유번호 :HP20C0194 ).

References

  1. Ahn, S.I., B.J. Heuing, and J.Y.Son. 2007. Antioxidative activity and nitrite scavenging abilities of some phenolic compounds. Kor. J. Food Cookery Sci. 23(1): 19-24.
  2. Al-Hashimi, A.G. 2012. Antioxidant and antibacterial activities of Hibiscus sabdariffa L. extracts. Afri. J. Food Sci. 6(21): 506-511. doi: 10.5897/AJFS12.099
  3. Anwar, F., S. Latif, M. Ashraf, and A.H. Gilani. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Phytother. Res. 21(1): 17-25. doi: 10.1002/ptr.2023
  4. Bae, G.S. and S.J. Park. 2016. The Anti-inflammatory Effect of Nypa fruticans Wurmb. Fruit on Lipopolysaccharide-induced Inflammatory response on RAW 264.7 cells. Kor. J. Herbol. 31(5): 79-84. doi: 10.6116/kjh.2016.31.5.79.
  5. Da-Costa-Rocha, I., B. Bonnlaender, H. Sievers, I. Pischel, and M. Heinrich. 2014. Hibiscus sabdariffa L.-A phytochemical and pharmacological review. Food Chem. 165(2): 424-443. doi: 10.1016 /j.foodchem.2014.05.002 https://doi.org/10.1016/j.foodchem.2014.05.002
  6. Deby, C., and R. Goutier. 1990. New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutases. Biochem. Pharmacol. 39(3): 399-405. doi: 10.1016/0006-2952(90)90043-k
  7. Faraji, M.H. and A.H. Tarkhani. 1999. The effect of sour tea (Hibiscus sabdariffa) on essential hypertension. J. Ethnopharmacol. 65(3): 231-236. doi: 10.1016/S0378-8741(98)00157-3
  8. Gopalakrishnan, L., K. Doriya, and D.S. Kumar. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci. Human Wellness. 5(2): 49-56. doi: 10.1016/j.fshw.2016.04.001
  9. Haenen, G.R., J.B. Paquay, R.E. Korthouwer, and A. Bast. 1997. Peroxynitrite scavenging by flavonoids. Biochem. Biophys. Res. Commun. 236(1): 591-593.doiI: 10.1006/bbrc.1997.7016
  10. Hamza, A.A. 2009. Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats. Food Chem. Toxicol. 48(1): 345-355. doi: 10.1016/j.fct.2009.10.022
  11. Hetog, M.G.L., P.H.C. Hollman, and B. van der outte. 1993. Content of potentially anticarcinogenic flavonoids of tea influsions, wines, and fruits juices. J. Agr. Food Chem. 41(8): 1242-1246. doi: 10.1021/jf00032a015
  12. Holvoet P. 2008. Relations between metabolic syndrome, oxidative stress and inflammation and cardiovascular disease. Verh. K. Acad. Geneeskd. Belg. 70(3): 193-219. PMID: 18669160
  13. Homan, R., J.E. Grossman, and H. Pownall. 1991. Differential effects of eicosapentaenoic acid and oleic acid on lipid synthesis and secretion by HepG2 cells. J. Lipid Res. 32(2): 231-241. PMID: 2066660 https://doi.org/10.1016/S0022-2275(20)42084-X
  14. Hong, S.G., D.M. Jeong, K.Y. Kim, and E.H. Hwang. 2010. The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract. Korean J. Nutr. 43(2): 105~113.doi: 10.4163/kjn.2010.43.2.105
  15. Hossain, F. and A. Islam. 2015. Utilization of mangrove forest plant: Nipa palm (Nypa fruticans Wurmb.). Am. J. Agri. Forest. 3(4):156-160. doi: 10.11648/j.ajaf.20150304.16
  16. Jian, S., J. Ban, H. Ren, and H. Yan. 2010. Low genetic variation detected within the widespread mangrove species Nypa fruticans (Palmae) from Southeast Asia. Aquat. Bot. 92(1): 23-27. doi: 10.1016/j.aquabot.2009.09.003
  17. Jung, E., Y. Kim, and N. Joo. 2013. Physicochemical properties and antimicrobial activity of Roselle (Hibiscus sabdariffa L.). J. Sci. Food Agric. 93(15): 3769-3776. DOI: 10.1002/jsfa.6256
  18. Kou, X., B. Li, J.B. Olayanju, J.M. Drake, and N. Chen. 2018. Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients 10(3): 1-12. doi: 10.3390/nu10030343
  19. Lee, S.G. and H. Kang. 2018. Effect of Ethanol Extracts from Defatted Perilla frutescens on LPS-induced Inflammation in Mouse BV2 Microglial Cells. Biomedical Science Letters. 24(4): 398-404. doi: 10.15616/BSL.2018.24.4.398
  20. Lin, M., J. Zhang, and X. Chen. 2018. Bioactive flavonoids in Moringa oleifera and their health-promoting properties. J. Funct. Foods. 47(2): 469-479. doi: 10.1016/j.jff.2018.06.011
  21. Onyenekwe, P.C., E.O. Ajani, D.A. Ameh, and K.S. Gamaniel. 1999. Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats. Cell Biochem. Funct. 17(3): 199-206. doi: 10.1002/(SICI)1099-0844(199909)17:3<199::AID-CBF829>3.0.CO;2-2
  22. Park, Y.S. 2002. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J. East Asian Soc. Diet. Life. 12(1): 23-31.
  23. Prasad, N., B. Yang, K.W. Kong, H.E. Khoo, J. Sun, A. Azlan, A. Ismail, and Z.B. Romli. 2013. Phytochemicals and antioxidant capacity from Nypa fruticans Wurmb. Fruit. Evid. Based Complement. Alternat. Med. 2013: 1-9. doi: 10.1155/2013/154606
  24. Rho, K.A., G.J. Kim, H.A. Ji, H.S. Lim, K.H. Chung, K.J. Lee, B.C. Song, and J.H. An. 2015. Antitumor and Free Radical-Scavenging Activities of Various Extract Fractions of Fruits and Leaves from Prunus mume. J. Kor. Soc. Food Sci. Nutr. 44(8): 1137-1143. doi: 10.3746/jkfn.2015.44.8.1137
  25. Sato, H., and S. Sakamura. 1975. Isolation and identification of flavonoids in immature buckwheat seed (Fagopyrum esculentum Monch). Agric. Chem. Soc. Jpn. 49(1): 53-55. doi: 10.1271/nogeikagaku1924.49.53
  26. Siddhuraju, P. and K. Becker. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem. 51(8): 2144-2155. doi: 10.1021/jf020444+
  27. Sreelatha, S., A. Jeyachitra, and P.R. Padma. 2011. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem. Toxicol. 49(6): 1270-1275. doi: 10.1016/j.fct.2011.03.006
  28. Sugai, K., S. Watanabe, T. Kuishi, S. Imura, K. Ishigaki, M. Yokota, S. Yanagawa, and Y. Suyama. 2016. Extremely low genetic diversity of the northern limit populations of Nypa fruticans (Arecaceae) on Iriomote Island, Japan. Conserv. Genet. Resour. 17(1): 221-228. doi: 10.1007/s10592-015-0773-6
  29. Tamunaidu, P. and S. Saka. 2011. Chemical characterization of various parts of nipa palm (Nypa fruticans). Ind. Crop Prod. 34(3): 1423-1428. doi: 10.1016/j.indcrop.2011.04.020
  30. Yang, L., Y. Gou, T. Zhao, J. Zhao, F. Li, B. Zhang, and X. Wu. 2012. Antioxidant capacity of extracts from calyx fruits of roselle (Hibiscus sabdariffa L.). African J. Biotechnol. 11(17): 4063-4068. doi: 10.5897/AJB11.2227
  31. Yosoff, N.A., M. Ahmad, B. AI-Hindi, T. Widyawati, M.F. Yam, and R. Mahmud. 2015. Aqueous extract of Nypa fruticans Wurmb. Vinegar alleviates postprandial hyperglycemia in normoglycemic rats. Nutrients. 7(8): 7012-7026. doi: 10.3390/nu7085320