Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of UV-C irradiation for bacterial disinfection of drinking water

UV-C 조사에 의한 음용수 살균 시스템의 미생물 저해 효과

  • Kim, Hyun-Joong (Department of Food Engineering, Mokpo National University) ;
  • Kim, Yun-Jae (Department of Food Engineering, Mokpo National University) ;
  • Seo, Se-Yeong (Department of Food Engineering, Mokpo National University) ;
  • Lee, Chang Joo (Department of Food Science and Biotechnology, Wonkwang University)
  • 김현중 (목포대학교 식품공학과) ;
  • 김윤재 (목포대학교 식품공학과) ;
  • 서세영 (목포대학교 식품공학과) ;
  • 이창주 (원광대학교 식품생명공학과)
  • Received : 2021.03.22
  • Accepted : 2021.04.05
  • Published : 2021.04.30
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Abstract

This study aimed to investigate bacterial disinfection in drinking water using a water purifier. Water artificially inoculated with Escherichia coli and Listeria monocytogenes at various concentrations was irradiated using ultraviolet (UV)-C at a rate of 3.4 L/min in a water purifier, and the disinfection effects of UV-C were evaluated. Both E. coli and L. monocytogenes were disinfected up to 107 colony-forming units (CFU)/2.8 L by the UV-C irradiation. Additionally, morphological study using fluorescence microscopy in conjunction with live/dead staining revealed that both the bacteria species were disinfected by the UV-C irradiation. Therefore, UV-C in water purifiers can effectively kill high concentrations of bacteria in distilled water. UV irradiation (UV-C: 254 nm wavelength, irradiation dose: 40 mJ/㎠) at a flow rate of 3.4 L/min on drinking water has the potential to sterilize bacteria-contaminated drinking water, at least for 3.2×107 CFU/2.8 L of E. coli and 8.4×107 CFU/2.8 L of L. monocytogenes.

본 연구는 그람 음성균과 양성균인 E. coli와 L. monocytogenes를 인위적으로 오염시킨 음용수를 대상으로 UV-C 정수기의 살균 가능성과 미생물의 생육 저해에 따른 형태학적 특성을 조사하였다. UV-C를 이용한 음용수 살균 능력은 그람 음성균 E. coli와 양성균 L. monocytogenes의 저농도부터 고농도(E. coli 3.2×103-3.2×107 CFU/2.8 L; L. monocytogenes 8.4×103-8.4×107 CFU/2.8 L) 세균 모두의 생육을 저해할 수 있는 효과가 나타났다. 따라서 UV를 이용한 음용수 살균은 유속 3.4 L/min에서 UV-C 파장 254 nm, 조사선량 40 mJ/㎠로 조사할 경우 E. coli 3.2×107 CFU/2.8 L와 L. monocytogenes 8.4×107 CFU/2.8 L 이하 농도의 오염된 음용수의 살균이 가능한 것으로 나타났다. 정수기에 UV-C 살균장치를 추가하는 것이 물의 미생물 안전성에 효율적이라고 사료되며, 이 연구결과는 UV살균 장치 활용의 기초자료로 제공되어 향후 연구 수행에 도움을 줄 수 있을 것이다.

Keywords

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